KoreanCurcuma longa L. induces lipolysis and regulates leptin in adipocyte cells and rats

Abstract

BACKGROUND/OBJECTIVESTurmeric (Curcuma longa L.) has been reported to have many biological functions including anti-obesity. Leptin, peptide hormone produced by adipocytes and its concentration is increased in proportion to the amount of the adipocytes. In the present study, we examined the effects of Korean turmeric on the regulation of adiposity and leptin levels in 3T3-L1 adipocytes and rats fed a high-fat and high-cholesterol diet.MATERIALS/METHODSLeptin secretion, free fatty acid and glycerol contents in 3T3-L1 adipocytes were measured after incubation of cells with turmeric for 24 hours. Rats were divided into four experimental groups: a normal diet group (N), a high-fat and high-cholesterol diet group (HF), a high-fat and high-cholesterol diet group supplemented with 2.5% turmeric extracts (TPA group) and a high-fat and high-cholesterol diet group supplemented with 5% turmeric extracts (TPB group). Serum samples were used for the measurement of leptin concentration.RESULTSContents of free fatty acid and glycerol showed concentration dependent increase in response to turmeric extracts. Effects of turmeric extracts on reduction of lipid accumulation in 3T3-L1 cells were examined by Oil Red O staining. Treatment with turmeric extracts resulted in increased expression levels of adipose triglyceride lipase and hormone-sensitive lipase mRNA. The concentration of leptin from 3T3-L1 adipocytes was significantly decreased by turmeric. Proportional abdominal and epididymal fats weights of the turmeric 5% supplemented group, TPB has significantly decreased compared to the HF group. The serum levels of leptin in the TPA and TPB groups were significantly lower than those of the HF group.CONCLUSIONSBased on these results, we suggested that Korean turmeric may contribute to the decreasing of body fat and regulating leptin secretion.

Curcuminoids Lower Plasma Leptin Concentrations: A Meta‐analysis

“…Leptin activates inflammatory cytokine release, including IL-1a, IL-1b, IL-6, and TNF-α, that in turn modulates the immune response (Lord et al, 1998). Adiponectin and gherelin are also important in appetite and energy homeostais, and curcumin has been shown to cause adipokine release from adipocytes (Panahi et al, 2016b), inducing lipolysis that may affect leptin (Song and Choi, 2016). It has been suggested that curcumin may increase ghrelin levels through its antioxidant actions (Xu et al, 2013); however, how curcumin modulates the interplay between leptin, adiponectin, and gherelin needs clarification.…”

“…Investigative clinical trials have suggested that curcumin has antioxidant (Panahi et al, 2016a;Sahebkar et al, 2015b), antiinflammatory (Panahi et al, 2012;Sahebkar, 2014a), and immunomodulatory (Ghandadi and Sahebkar, 2017;Karimian et al, 2017;Ganjali et al, 2014) properties and is effective in a wide number of diverse conditions including osteoarthritis and rheumatoid arthritis (Kurien et al, 2015;Panahi et al, 2014c;Sahebkar and Henrotin, 2016), metabolic syndrome (Inzaugarat et al, 2017;Panahi et al, 2015b;Panahi et al, 2014b;Sahebkar, 2013;Rahmani et al, 2016), cancer (Deng et al, 2016;Teymouri et al, 2017;Mirzaei et al, 2016;Momtazi et al, 2016), dyslipidemia (Panahi et al, 2016c;Sahebkar, 2014b;Ganjali et al, 2017), chronic obstructive pulmonary disease (Lelli et al, 2017;Panahi et al, 2014a), and anxiety and depression (Esmaily et al, 2015;Panahi et al, 2015a). Curcumin has a direct effect on adipokine release from adipocytes (Panahi et al, 2016b;Hajavi et al, 2017) with the suggestion that it may induce lipolysis and regulate leptin in animals (Song and Choi, 2016). Data suggest that there is an effect on leptin receptor gene expression (Nejati-Koshki et al, 2014b;Nejati-Koshki et al, 2014a) and that interruption of the leptin signaling pathway with a fall in leptin levels decreases advanced glycation end-products (Tang and Chen, 2014).…”

Antidiabetic Properties of Curcumin I: Evidence from In Vitro Studies

“…In a study by Song and Choi (2016), treatment of 3T3-L1 adipocytes with 10 µg/mL turmeric extracts for 24 h resulted in significantly reduced lipid accumulation and leptin levels, while the levels of free fatty acids and glycerol were increased [61]. In addition, turmeric treatment increased adipose triglyceride lipase and hormone-sensitive lipase mRNA levels, suggesting increased triglyceride degradation ( Table 1) [61].…”

Anti-adipogenic effect of a turmeric extract-loaded nanoemulsion in 3T3-L1 preadipocytes and high fat diet-fed mice

“…An increase in the number of adipocytes (hyperplasia) due to adipocyte differentiation and an increase in the size of adipocytes (hypertrophy) are both contributors to the development of obesity (Guo et al, 2015;Shepherd et al, 1993;Spalding et al, 2008). An increase in adipose tissue mass is paralleled by higher prevalence of obesity-related complications, such as hyperlipidemia, hypertension, type 2 diabetes, and nonalcoholic fatty liver disease (Kopelman, 2000;Spalding et al, 2008). An improved understanding of adipocyte differentiation and function is critical to accelerate the development of drugs for the treatment of obesity and its complications.…”