Kribbella karoonensis sp. nov. and Kribbella swartbergensis sp. nov., isolated from soil from the Western Cape, South Africa

Abstract

Two novel nocardioform actinomycetes, strains Q41T and HMC25T, were isolated from soil samples collected in the Western Cape province, South Africa. Rapid genus identification revealed that the isolates belonged to the genus Kribbella (based on single-digestion restriction analysis of the 16S rRNA gene sequences with MboI, VspI, SphI, SnaBI, SalI and AgeI). Both isolates had ll-diaminopimelic acid and glycine in their cell-wall peptidoglycan, and contained mannose and ribose as whole-cell sugars. Strain HMC25T is able to grow at 45 °C and in the presence of NaCl (3 %), cephaloridine (10 μg ml−1) and gentamicin sulphate (10 μg ml−1). Strain Q41T grows in the presence of NaCl (2 %). Neither strain was able to grow under anaerobic conditions, whereas Kribbella flavida KACC 20248T, Kribbella jejuensis HD9T, Kribbella koreensis KACC 20250T and Kribbella sandramycini KACC 20249T exhibited weak but distinct growth under anaerobic conditions. Physiological test results and 16S rRNA gene sequence analysis allowed Q41T and HMC25T to be distinguished from other members of the genus with validly published names. Strains HMC25T (=NRRL B-24426T=DSM 17345T) and Q41T (=NRRL B-24425T=DSM 17344T) therefore represent the type strains of novel species, for which the names Kribbella swartbergensis sp. nov. and Kribbella karoonensis sp. nov., respectively, are proposed.

Phylogenetic analysis of the genus Kribbella based on the gyrB gene: proposal of a gyrB-sequence threshold for species delineation in the genus Kribbella

“…Kribbella hippodromi S1-4 T , Kribbella karoonensis Q41 T , Kribbella swartbergensis HMC25 T and Kribbella solani strain YB2 were isolated by us (Everest and Meyers 2008;Kirby et al 2006). The two K. aluminosa non-type strains (HKI-479 and HKI-480;Carlsohn et al 2007) were obtained from Drs Ingrid Groth and Karin Martin.…”

Kribbella hippodromi sp. nov., isolated from soil from a racecourse in South Africa

“…Table 1. Differential phenotypic characteristics between strain S1.4 T and closely related Kribbella type strains Strain: 1, S1.4 T ; 2, K. solani DSA1 T ; 3, K. swartbergensis HMC25 T (Kirby et al, 2006); 4, K. jejuensis HD9 T (Song et al, 2004); 5, K. aluminosa HKI 0478 T (Carlsohn et al, 2007). Conflicting data are indicated in parentheses.…”

“…With the transfer of Hongia koreensis to the genus (Sohn et al, 2003) and more recently the descriptions of Kribbella karoonensis and Kribbella swartbergensis (Kirby et al, 2006) and Kribbella aluminosa (Carlsohn et al, 2007) there are, at the time of writing, 12 recognized Kribbella species (Euzéby, 2007). Here we describe the characterization of a novel member of the genus, isolated from a soil sample collected from a racecourse in the Western Cape, South Africa.…”

Kribbella catacumbae sp. nov. and Kribbella sancticallisti sp. nov., isolated from whitish-grey patinas in the catacombs of St Callistus in Rome, Italy

“…species of Kribbella have been described: Kribbella flavida, Kribbella sandramycini (Park et al, 1999), Kribbella solani and Kribbella jejuensis (Song et al, 2004), Kribbella antibiotica (Li et al, 2004), Kribbella lupini (Trujillo et al, 2006), Kribbella yunnanensis and Kribbella alba (Li et al, 2006), and Kribbella karoonensis and Kribbella swartbergensis (Kirby et al, 2006). Kribbella koreensis, described first as Hongia koreensis by Lee et al (2000), was transferred to the genus Kribbella by Sohn et al (2003).…”