Kruppel-like factor 4-dependent Staufen1-mediated mRNA decay regulates cortical neurogenesis

Moon, Byoung-San; Bai, Jinlun; Cai, Mingyang; Liu, Chunming; Shi, Jiandang; Lu, Wange

doi:10.1038/s41467-017-02720-9

Cited by 37 publications

(31 citation statements)

References 58 publications

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“…The isolated spiral ganglion cells were cultured in N2 medium containing basic fibroblast growth factor (bFGF; 20 ng/mL, PeproTech, Rocky Hill, NJ, USA) for 4 days to maintain stem cell characteristics and then evaluated for Nestin and Sox2 expression. To induce differentiation, the cells were seeded and further cultured in bFGF depleted medium [ 11 - 13 ]. Neurosphere culturing was conducted as has been described previously [ 11 - 13 ].…”

Section: Methodsmentioning

confidence: 99%

“…To induce differentiation, the cells were seeded and further cultured in bFGF depleted medium [ 11 - 13 ]. Neurosphere culturing was conducted as has been described previously [ 11 - 13 ].…”

Section: Methodsmentioning

confidence: 99%

“…Immunohistochemistry and immunocytochemistry was conducted as has been described previously [ 11 - 13 ]. Briefly, postnatal mice (P1, 2 weeks old) were euthanized with carbon dioxide.…”

Section: Methodsmentioning

confidence: 99%

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The Presence of Neural Stem Cells and Changes in Stem Cell-Like Activity With Age in Mouse Spiral Ganglion Cells In Vivo and In Vitro

Moon

Ammothumkandy

Zhang

et al. 2018

Clin Exp Otorhinolaryngol

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ObjectivesSpiral ganglion neurons (SGNs) include potential endogenous progenitor populations for the regeneration of the peripheral auditory system. However, whether these populations are present in adult mice is largely unknown. We examined the presence and characteristics of SGN-neural stem cells (NSCs) in mice as a function of age.MethodsThe expression of Nestin and Ki67 was examined in sequentially dissected cochlear modiolar tissues from mice of different ages (from postnatal day to 24 weeks) and the sphere-forming populations from the SGNs were isolated and differentiated into different cell types.ResultsThere were significant decreases in Nestin and Ki67 double-positive mitotic progenitor cells in vivo with increasing mouse age. The SGNs formed spheres exhibiting self-renewing activity and multipotent capacity, which were seen in NSCs and were capable of differentiating into neuron and glial cell types. The SGN spheres derived from mice at an early age (postnatal day or 2 weeks) contained more mitotic stem cells than those from mice at a late age.Conclusion.Our findings showed the presence of self-renewing and proliferative subtypes of SGN-NSCs which might serve as a promising source for the regeneration of auditory neurons even in adult mice.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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The Presence of Neural Stem Cells and Changes in Stem Cell-Like Activity With Age in Mouse Spiral Ganglion Cells In Vivo and In Vitro

Moon

Ammothumkandy

Zhang

et al. 2018

Clin Exp Otorhinolaryngol

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“…It is a chronic metabolic disease caused by the interaction of genetic and environmental factors [1,24]. A large number of studies have shown that STAU1 can regulate cell growth and nerve differentiation [25]. However, little is known about the post-transcriptional regulation of adipogenesis by STAU1.…”

Section: Discussionmentioning

confidence: 99%

Regulation of Klf16 by Double Strand RNA-binding Protein STAU1 in Adipocyte Differentiation in vitro

Guan

Meng

Liang

et al. 2020

Preprint

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Background: Adipogenesis is an essential process in organismal development and plays a significant role in adipose tissue homeostasis. Post-transcriptional regulation of gene expression plays a key role in adipogenesis and involves many RNA-binding proteins (RBPs). In mammals, Staufen1 (STAU1) is a conserved RBP(RNA Binding Protein )consisting of several dsRBP (double strand RNA). STAU1 plays an important role in the Stau1-mediated mRNA decay (SMD) pathway, which is related to adipocyte formation, myocyte development, and neural differentiation. Klf16 (Kruppel like transcription factor 16) is a negative regulator that inhibits adipocyte differentiation. AIM:This study was conducted to determine the role of Klf16 in adipocyte differentiation in the context of the SMD pathway.Methods: 3T3-L1 cells were induced and cultured in vitro by cocktail method, Knockdown and Overexpression of STAU1 and KLF16. Then, adipocyte differentiation andexpression of adipogenic-related genes (STAU1, KLF16, PPARγ, and Lipin1) were measured by RT-qPCR and Western blot.RNA immunoprecipitation (RIP) method verified that STAU1 protein can bind to KLF16.Results: The results revealed that STAU1 regulates Klf16 expression at the post-transcriptional level during the adipogenic differentiation of 3T3-L1 cells.STAU1 candirectly bind the 3′UTR of Klf16 mRNA. Klf16 mRNA was found to be degraded through the SMD pathway, thus promoting adipocyte differentiation.Conclusions: In this study, the mechanism of adipocyte differentiation regulation at the post-transcriptional level is demonstrated, and Klf16 is shown as a substrate of the SMD pathway, thus providing new insights into adipogenesis.

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“…Dusp1 has been implicated in neuroprotection [41,42] and has been linked with diabetes related cognitive impairment [43] . Klf4 is involved in neurite growth and regeneration in hippocampal and cortical neurons, and its dysregulation has been linked to various neurological disorders [44,45] .…”

Section: Discussionmentioning

confidence: 99%

Multi-tissue Multi-omics Nutrigenomics Indicates Context-specific Effects of DHA on Rat Brain

Zhang

Meng

Blencowe³

et al. 2020

Preprint

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ScopeWe explored the influence of DHA on cardiometabolic and cognitive phenotypes, and multiomic alterations in the brain under two metabolic conditions to understand context-specific nutritional effects.Methods and ResultsRats were randomly assigned to a DHA-rich or a control chow diet while drinking water or high fructose solution, followed by profiling of metabolic and cognitive phenotypes and the transcriptome and DNA methylome of the hypothalamus and hippocampus. DHA reduced serum triglyceride and improved insulin resistance and memory exclusively in the fructose-consuming rats. In hippocampus, DHA affected genes related to synapse functions in the chow group but immune functions in the fructose group; in hypothalamus, DHA altered immune pathways in the chow group but metabolic pathways in the fructose group. Network modeling revealed context-specific regulators of DHA effects, including Klf4 and Dusp1 for chow condition and Lum, Fn1, and Col1a1 for fructose condition in hippocampus, as well as Cyr61, JunB, Ier2, and Pitx2 under chow condition and Hcar1, Cdh1, and Osr1 under fructose condition in hypothalamus.ConclusionDHA exhibits differential influence on epigenetic loci, genes, pathways, and metabolic and cognitive phenotypes under different dietary contexts, supporting population stratification in DHA studies to achieve precision nutrition.

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Kruppel-like factor 4-dependent Staufen1-mediated mRNA decay regulates cortical neurogenesis

Cited by 37 publications

References 58 publications

The Presence of Neural Stem Cells and Changes in Stem Cell-Like Activity With Age in Mouse Spiral Ganglion Cells In Vivo and In Vitro

The Presence of Neural Stem Cells and Changes in Stem Cell-Like Activity With Age in Mouse Spiral Ganglion Cells In Vivo and In Vitro

Regulation of Klf16 by Double Strand RNA-binding Protein STAU1 in Adipocyte Differentiation in vitro

Multi-tissue Multi-omics Nutrigenomics Indicates Context-specific Effects of DHA on Rat Brain

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