Krüppel-Like Factor 6 Rendered Rat Schwann Cell More Sensitive to Apoptosis via Upregulating FAS Expression

Gui, Ting; Wang, Yueming; Zhang, Lixing; Wang, Wenjing; Zhu, Haijing; Ding, Wenlong

doi:10.1371/journal.pone.0082449

Cited by 17 publications

(11 citation statements)

References 42 publications

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“…Real-time PCR were processed according to our previous protocols [ 19 ]. Briefly, astrocytes were incubated in serum-free DMEM containing 0, 5 or 20 μM MK-801 for 24 h and washed with PBS.…”

Section: Methodsmentioning

confidence: 99%

Reactive Transformation and Increased BDNF Signaling by Hippocampal Astrocytes in Response to MK-801

Zhu

Wang

et al. 2015

PLoS ONE

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MK-801, also known as dizocilpine, is a noncompetitive N-methyl-D-aspartic acid (NMDA) receptor antagonist that induces schizophrenia-like symptoms. While astrocytes have been implicated in the pathophysiology of psychiatric disorders, including schizophrenia, astrocytic responses to MK-801 and their significance to schizotypic symptoms are unclear. Changes in the expression levels of glial fibrillary acid protein (GFAP), a marker of astrocyte activation in response to a variety of pathogenic stimuli, were examined in the hippocampus of rats treated with the repeated MK-801 injection (0.5 mg/10ml/kg body weight for 6 days) and in primary cultured hippocampal astrocytes incubated with MK-801 (5 or 20 μM for 24 h). Moreover, the expression levels of BDNF and its receptors TrkB and p75 were examined in MK-801-treated astrocyte cultures. MK-801 treatment enhanced GFAP expression in the rat hippocampus and also increased the levels of GFAP protein and mRNA in hippocampal astrocytes in vitro. Treatment of cultured hippocampal astrocytes with MK-801 enhanced protein and mRNA levels of BDNF, TrkB, and p75. Collectively, our results suggest that hippocampal astrocytes may contribute to the pathophysiology of schizophrenia symptoms associated with NMDA receptor hypofunction by reactive transformation and altered BDNF signaling.

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Section: Methodsmentioning

confidence: 99%

Reactive Transformation and Increased BDNF Signaling by Hippocampal Astrocytes in Response to MK-801

Zhu

Wang

et al. 2015

PLoS ONE

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“…We found that the antiapoptosis protein Bcl2 was upregulated in NRBP2 low cells, whereas the proapoptosis protein Bax was downregulated. Bax/ Bcl2 ratio could reflect the propensity or resistance to apoptosis (25). Then, Bax/Bcl2 ratio was calculated and the results displayed that the Bax/Bcl2 ratio was decreased in the NRBP2 low cells (Fig.…”

Section: Nrbp2 Inhibits Akt Phosphorylation and Regulates Bcl2 Familymentioning

confidence: 97%

NRBP2 Overexpression Increases the Chemosensitivity of Hepatocellular Carcinoma Cells via Akt Signaling

Zhang

Zhao

et al. 2016

Cancer Research

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Hepatocellular carcinoma is highly resistant to chemotherapy. Research data supported that cancer stem cells (CSC) may be responsible for the chemoresistance and strategies that suppress CSCs stemness could also inhibit the drug resistance. In this study, we found that nuclear receptor binding protein 2 (NRBP2) expression was downregulated in the CD133 hepatocellular carcinoma CSCs. Most adjacent noncancerous liver tissue analyzed expressed higher level of NRBP2 compared with cancerous tissue in hepatocellular carcinoma patients, and high NRBP2 expression indicated a better prognosis. Real-time PCR results showed that NRBP2 negatively correlated with stemness-related genes, including Oct3/4, Nanog, Notch1, Ep300, and CD133 mRNA expression. High NRBP2 expression in hepatocellular carcinoma cells downregulated CK19 protein expression, inhibited tumorsphere formation, and tumorigenesis ability, indicating that high NRBP2 expression restrains the hepatocellular carcinoma cell stemness. Overexpression of NRBP2 reduced the IC of sorafenib in hepatocellular carcinoma cells, and NRBP2 expression was negatively correlated with hepatocellular carcinoma cell resistance to the chemotherapy agents, including cisplatin and the Akt signaling inhibitor perifosine. Coimmunoprecipitation results showed that NRBP2 could bind with Annexin A2 (ANXA2) and inhibit ANXA2 expression. Coexpression of ANXA2 restored the chemoresistant ability in NRBP2-overexpressing hepatocellular carcinoma cells. Further analysis showed that NRBP2 downregulated Akt and its downstream signaling target Bad phosphorylation level. ANXA2 coexpression partially restored the Akt phosphorylation. Analysis of the expression of Bcl2 family proteins showed that NRBP2 may increase hepatocellular carcinoma cell chemosensitivity by regulating expression of survival proteins involved in the Akt and Bcl2 pathway. These results suggest that NRBP2 plays an important role in the tumor progression and chemotherapeutic resistance of hepatocellular carcinoma. Cancer Res; 76(23); 7059-71. ©2016 AACR.

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“…The results are responsible for three independent experiments Cell Mol Neurobiol In higher vertebrates, the capacity of the PNS to regenerate is robust compared to that of the CNS. The regeneration potential of peripheral nerve may due to the proliferation and activation of Schwann cells, which produce growth factors that promote axonal regrowth at the proximal nerve stump and provide a growth-permissive condition for axon guidance (Gui et al 2013;Hall 2001). Although the role of PCBP2 in the PNS has not been reported before our research, many studies focused on the function of PCBP2 gene in the CNS and many other diseases.…”

Section: Discussionmentioning

confidence: 78%

Spatiotemporal Expression of Poly(rC)-Binding Protein PCBP2 Modulates Schwann Cell Proliferation After Sciatic Nerve Injury

Chen

Zhang

et al. 2015

Cell Mol Neurobiol

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Poly(C)-binding proteins (PCBPs), also known as RNA-binding proteins, interact in a sequence-specific fashion with single-stranded poly(C). It was reported that PCBP2 contributed to gastric cancer proliferation and survival through miR-34a, and knockdown of PCBP2 inhibited glioma proliferation through inhibition of cell cycle progression. In addition, PCBP2 might play a critical role in the regulation of cortical neurons apoptosis induced by hypoxia or ischemia. Because of the essential role of PCBP2 in nervous system and cell growth, we investigated the spatiotemporal expression of PCBP2 in a rat sciatic nerve crush (SNC) model. We detected the upregulated expression of PCBP2 in Schwann cell after SNC. Besides, the peak expression of PCBP2 was in parallel with proliferation cell nuclear antigen. In vitro, we observed increased expression of PCBP2 during the process of TNF-α-induced Schwann cell proliferation. Specially, PCBP2-specific siRNA-transfected Schwann cell showed significantly decreased ability for proliferation. Together, all these data indicated that the change of PCBP2 protein expression was associated with Schwann cell proliferation after the trauma of the peripheral nervous system.

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Krüppel-Like Factor 6 Rendered Rat Schwann Cell More Sensitive to Apoptosis via Upregulating FAS Expression

Cited by 17 publications

References 42 publications

Reactive Transformation and Increased BDNF Signaling by Hippocampal Astrocytes in Response to MK-801

Reactive Transformation and Increased BDNF Signaling by Hippocampal Astrocytes in Response to MK-801

NRBP2 Overexpression Increases the Chemosensitivity of Hepatocellular Carcinoma Cells via Akt Signaling

Spatiotemporal Expression of Poly(rC)-Binding Protein PCBP2 Modulates Schwann Cell Proliferation After Sciatic Nerve Injury

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