Spatiotemporal Expression of Poly(rC)-Binding Protein PCBP2 Modulates Schwann Cell Proliferation After Sciatic Nerve Injury

Chen, Zhigang; Zhang, Weidong; Ni, Li; Wang, Genlin; Cao, Yi; Wu, Wenyan; Sun, Chi; Yuan, Damin; Ni, Haidan; Wang, Youhua; Yang, Huilin

doi:10.1007/s10571-015-0253-z

Cited by 8 publications

(3 citation statements)

References 32 publications

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“…In present study, a significant increase of LINC02535 and PCBP2 expression was found in CC tissues and cells compared with the adjacent normal tissues and normal cervical epithelial cells. Research has indicated that PCBP2 plays an important role in promoting the occurrence and development of many kinds of cancers (Chen et al, 2016;Tang, Gao, & Chen, 2015;Zhang et al, 2016). Through starBase website, there was a potential binding site between LINC02535 and PCBP2.…”

Section: Discussionmentioning

confidence: 99%

LINC02535 co‐functions with PCBP2 to regulate DNA damage repair in cervical cancer by stabilizing RRM1 mRNA

Wen

Huang

et al. 2020

Journal Cellular Physiology

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Cervical cancer (CC) is one of the commonest malignant cancers among women with high morbidity and mortality. Despite encouraging advances had been found in diagnostic and therapeutic strategies, effective therapeutic strategy and further exploration of the mechanism underlying in CC is still needed. We searched The Cancer Genome Atlas database and found that long noncoding RNA LINC02535 was highly expressed in CC. LINC02535 has not been studied in CC, and its molecular regulation mechanism remains unknown. Based on starBase database, LINC02535 could potentially bind poly (rC) binding protein 2 (PCBP2). In the present study, we discovered a significant increase of the LINC02535 and PCBP2 expression in CC tissues and cells as compared with the adjacent normal tissues and normal cervical epithelial cells. LINC02535 and PCBP2 can bind with each other and were colocated in cytoplasm. LINC02535 and PCBP2 promoted cell proliferation, migration, invasion, and suppressed apoptosis in CC. LINC02535 and PCBP2 facilitated the repair of DNA damage to promote CC progression. LINC02535 cooperated with PCBP2 to enhance the stability of RRM1 messenger RNA (mRNA). RRM1 promoted the repair of DNA damage and epithelial-to-mesenchymal transition (EMT) process in CC cells. LINC02535 regulated tumorigenesis in vivo. In conclusion, LINC02535 cooperated with PCBP2, regulated stability of RRM1 mRNA to promote cell proliferation and EMT process in CC cells by facilitating the repair of DNA damage, providing a potential biomarker for CC.

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Section: Discussionmentioning

confidence: 99%

LINC02535 co‐functions with PCBP2 to regulate DNA damage repair in cervical cancer by stabilizing RRM1 mRNA

Wen

Huang

et al. 2020

Journal Cellular Physiology

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“…Poly(C) binding protein 2 (PCBP2), a RNA-binding protein with 39 kDa, can regulate RNA stabilization, translational silencing and enhancement (Collier, Goobar-Larsson, Sokolowski, & Schwartz, 1998;Wan et al, 2016). It contains three K homology domains employed as the main RNA recognized regions (Chen et al, 2016;Leffers, Dejgaard, & Celis, 1995). Dysregulation of PCBP2 may damage multiple biological processes through RNAbinding pathways.…”

mentioning

confidence: 99%

PCBP2 promotes the development of glioma by regulating FHL3/TGF‐β/Smad signaling pathway

Mao

Sun

Cui

et al. 2019

Journal Cellular Physiology

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The purpose of this study was to investigate the role of Poly (C)-binding protein 2 (PCBP2) and the related signaling pathway in glioma progression. Quantitative realtime polymerase chain reaction (qRT-PCR) and immunohistochemistry (IHC) were performed to measure PCBP2 messenger RNA and protein expression in glioma tissues or cells. Cell transfection was completed using Lipofectamine 2000. 3-(4, 5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, Transwell assay and flow cytometry assay were used to explore the effects of PCBP2 expression on biological behaviors of glioma cells. Western blot assay was used for the detection of pathway related proteins. Expression of PCBP2 in glioma tissues and cells were higher than that in paracancerous tissues and normal cells (both p < .01). Moreover, the elevated expression of PCBP2 was significantly correlated with tumor size (p = .001) and WHO stage (p = .010). Knockdown of PCBP2 could suppress proliferation, migration and invasion of glioma cells and promote apoptosis. Besides, the expression of transforming growth factor-β (TGF-β) pathway related proteins TGF-β1, p-Smad2 and p-Smad7 were decreased following the downregulation of PCBP2. PCBP2 also inhibited FHL3 expression by binding to FHL3-3′UTR. The inhibition of FHL3 could reverse the antitumor action caused by PCBP2 silencing. In vivo assay, PCBP2 was also found to inhibit the tumor growth of glioma. PCBP2 activates TGF-β/Smad signaling pathway by inhibiting FHL3 expression, thus promoting the development and progression of glioma.

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“…Poly(rC) binding protein 2 (PCBP2), a 39 kDa protein, is a member of the a-globin mRNP stability complex [7,8]. As a generally known RNA-binding protein, PCBP2 possesses three K homology domains, which are the main RNA-recognition regions [9,10]. As a result of the RNA-binding characteristic, PCBP2 contributes to mRNA stabilization, translational silencing and enhancement [11,12].…”

mentioning

confidence: 99%

Poly(rC) binding protein 2 (PCBP2) promotes the viability of human gastric cancer cells by regulating CDK2

et al. 2018

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Survival rates for patients with gastric cancer, especially the advanced form, remain poor and the development of targeted treatments is hampered by a lack of efficient biological targets. Poly( rC ) binding protein 2 ( PCBP 2) is an RNA ‐binding protein that contributes to mRNA stabilization, translational silencing and enhancement and it has been implicated as a promoter of gastric cancer growth. In the present study, we demonstrated that the expression level of PCBP 2 was higher in human gastric cancer tissues compared to adjacent normal gastric tissues. A high level of PCBP 2 was correlated with worse postoperative relapse‐free survival and overall survival rates of gastric cancer patients. Small hairpin RNA ‐mediated depletion of PCBP 2 dramatically decreased the viability of gastric cancer cells. Cyclin‐dependent kinase 2 ( CDK 2) was positively regulated by PCBP 2 via a direct 3′ UTR binding pathway as determined using a ribonucleoprotein immunoprecipitation assay and a biotin pulldown assay. CDK 2 mediated the promoting role of PCBP 2. These results suggest that PCBP 2 acts as an oncogene in human gastric cancer cells and that functionally depleting PCBP 2 could be considered as a potential target for gastric cancer therapy.

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Spatiotemporal Expression of Poly(rC)-Binding Protein PCBP2 Modulates Schwann Cell Proliferation After Sciatic Nerve Injury

Cited by 8 publications

References 32 publications

LINC02535 co‐functions with PCBP2 to regulate DNA damage repair in cervical cancer by stabilizing RRM1 mRNA

LINC02535 co‐functions with PCBP2 to regulate DNA damage repair in cervical cancer by stabilizing RRM1 mRNA

PCBP2 promotes the development of glioma by regulating FHL3/TGF‐β/Smad signaling pathway

Poly(rC) binding protein 2 (PCBP2) promotes the viability of human gastric cancer cells by regulating CDK2

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