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Üçüncü, Sema Işisağ; Çakıcı, Özlem

doi:10.4194/trjfas.2009.0214

Cited by 14 publications

(3 citation statements)

References 37 publications

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“…As previously reported [15], the identification of atretic follicles (A) was based on atretic morphological markers characterized by Üçüncü and Çakıcı [43] such as granulosa cell hyperplasia, thinning, invagination and breakdowns of the zona radiata, basal membrane disintegration, and absorption of vitellus.…”

Section: Methodsmentioning

confidence: 99%

Characterization of Follicular Atresia Responsive to BPA in Zebrafish by Morphometric Analysis of Follicular Stage Progression

Migliaccio

Chioccarelli

Ambrosino

et al. 2018

International Journal of Endocrinology

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Bisphenol A is an industrial chemical compound, pervasively polluting the environment and diet, classified as an endocrine disruptor because of its interference effects on the endocrine system. In zebrafish, BPA exposure induces follicular atresia. To acquire knowledge on this atretic effect, using a qualitative and quantitative histomorphological approach, we studied zebrafish ovarian follicular stage development in response to low BPA concentrations. Results show that BPA interferes with follicular progression by affecting the previtellogenic and vitellogenic phases. In particular, BPA exposure (i) increases follicular recruitment by acting on primary stage follicles, (ii) forces the follicular transition from stage III to stage IV producing enlarged stage IV follicles, and (iii) induces atresia by producing atretic follicles that are peculiarly enlarged (i.e., big atretic follicles). We suggest that BPA induces atresia by the primary effect on recruitment of stage I follicles. This forces follicular progression and produces stage IV follicles that are peculiarly enlarged that undertake the atretic development.

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Section: Methodsmentioning

confidence: 99%

Characterization of Follicular Atresia Responsive to BPA in Zebrafish by Morphometric Analysis of Follicular Stage Progression

Migliaccio

Chioccarelli

Ambrosino

et al. 2018

International Journal of Endocrinology

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“…The follicles in dmrt1 −/− ;cyp19a1a −/− mutant zebrafish exhibited morphological features of atresia after 3 months, such as membrane disintegration, follicle cell proliferation and zona radiata breakdown ( Fig. S5) (Morais et al, 2012;Ucuncu and Cakici, 2009). The follicles at 100 dpf gradually underwent degeneration with interfollicular spaces becoming slowly occupied by stromal cells (Fig.…”

Section: Novel Insights On Roles Of Cyp19a1a/estrogens In Early Follimentioning

confidence: 97%

Disruption of dmrt1 rescues the all-male phenotype of cyp19a1a mutant in zebrafish – A Novel insight into the roles of aromatase/estrogens in gonadal differentiation and early folliculogenesis

Song

Zhang

et al. 2020

Development

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Sex determination and differentiation are complex processes controlled by many different factors; however, the relationships among these factors are poorly understood. Zebrafish gonadal differentiation exhibits high plasticity involving multiple factors and pathways, which provides an excellent model for investigating the interactions between them. Ovarian aromatase (cyp19a1a) and dmrt1 are key factors in directing vertebrate ovary and testis differentiation, respectively. Knockout of zebrafish cyp19a1a leads to all-male offspring, whereas the loss of dmrt1 results in a femalebiased sex ratio. In the present study, we established dmrt1 −/− ; cyp19a1a −/− double mutant zebrafish and discovered that the introduction of the dmrt1 mutation into the cyp19a1a mutant could rescue the all-male phenotype of the latter. Interestingly, despite the lack of aromatase/estrogens, the follicles in the ovary of the rescued cyp19a1a mutant could develop normally up to the previtellogenic stage. Further evidence suggested the ovarian aromatase directed ovarian differentiation by suppressing dmrt1 expression via nuclear estrogen receptors (nERs). Our results provide solid evidence for an interaction between cyp19a1a and dmrt1 in zebrafish gonadal differentiation, and for the dispensability of estrogens in controlling early folliculogenesis.

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“…Hyperplasia of granulosa cells; thinning, invasion, and breakdown of the zona radiata; disintegration of the basal membrane; and absorption of vitellus are the characteristics of atretic follicles . The ImageJ software was used to measure the follicular area.…”

Section: Methodsmentioning

confidence: 99%

The PCOS–NAFLD Multidisease Phenotype Occurred in Medaka Fish Four Generations after the Removal of Bisphenol A Exposure

Chakraborty

Anand

Coe

et al. 2023

Environ. Sci. Technol.

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As a heterogeneous reproductive disorder, polycystic ovary syndrome (PCOS) can be caused by genetic, diet, and environmental factors. Bisphenol A (BPA) can induce PCOS and nonalcoholic fatty liver disease (NAFLD) due to direct exposure; however, whether these phenotypes persist in future unexposed generations is not currently understood. In a previous study, we observed that transgenerational NAFLD persisted in female medaka for five generations (F4) after exposure to an environmentally relevant concentration (10 μg/L) of BPA. Here, we demonstrate PCOS in the same F4 generation female medaka that developed NAFLD. The ovaries contained immature follicles, restricted follicular progression, and degenerated follicles, which are characteristics of PCOS. Untargeted metabolomic analysis revealed 17 biomarkers in the ovary of BPA lineage fish, whereas transcriptomic analysis revealed 292 genes abnormally expressed, which were similar to human patients with PCOS. Metabolomic–transcriptomic joint pathway analysis revealed activation of the cancerous pathway, arginine–proline metabolism, insulin signaling, AMPK, and HOTAIR regulatory pathways, as well as upstream regulators esr1 and tgf signaling in the ovary. The present results suggest that ancestral BPA exposure can lead to PCOS phenotypes in the subsequent unexposed generations and warrant further investigations into potential health risks in future generations caused by initial exposure to EDCs.

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Untitled

Cited by 14 publications

References 37 publications

Characterization of Follicular Atresia Responsive to BPA in Zebrafish by Morphometric Analysis of Follicular Stage Progression

Characterization of Follicular Atresia Responsive to BPA in Zebrafish by Morphometric Analysis of Follicular Stage Progression

Disruption of dmrt1 rescues the all-male phenotype of cyp19a1a mutant in zebrafish – A Novel insight into the roles of aromatase/estrogens in gonadal differentiation and early folliculogenesis

The PCOS–NAFLD Multidisease Phenotype Occurred in Medaka Fish Four Generations after the Removal of Bisphenol A Exposure

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