Kryokonservierung von Blutzellen

Sputtek, Andreas

doi:10.1007/978-3-662-10599-3_8

Cited by 10 publications

(6 citation statements)

References 55 publications

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“…PBPC components were cryopreserved at a rate of −1°C per minute with a controlled cooling system. Below −60°C, PBPC components were transferred to the vapor phase of liquid nitrogen 17 …”

Section: Methodsmentioning

confidence: 99%

Processing of peripheral blood progenitor cell components in improved clean areas does not reduce the rate of microbial contamination

et al. 2002

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Section: Methodsmentioning

confidence: 99%

Processing of peripheral blood progenitor cell components in improved clean areas does not reduce the rate of microbial contamination

et al. 2002

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“…Those mechanisms destroy cell membrane before mechanical injury caused by ice crystals. This evolved into a generally accepted theory [6,10,11,19].…”

Section: Methods Of Cryopreservation Of Living Tissues and Cellsmentioning

confidence: 99%

Cryopreservation of Blood

Bohoněk¹

2012

Blood Transfusion in Clinical Practice

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“…It is known, that DMSO is a cytotoxic reagent when cells interfere with DMSO above freezing temperature [34,35]. We analyzed the time-dependent influence of DMSO in leukapheresis products on the determination of CD34+ cells.…”

Section: Cryoprotectant/cryopreservationmentioning

confidence: 99%

Factors Affecting the Determination of CD34+ Cells in Blood and Leukapheresis Products

Cassens¹,

Garritsen²,

Gutensohn³

et al. 1999

Transfus Med Hemother

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The number of peripheral blood stem cell (PBSC) transplantations permanently increased in recent years. Therefore, the determination of CD34+ cells in blood and leukapheresis products represents an elementary and obligatory technique for quantitation of PBSC. However, although the first national protocols for flow-cytometric determination of CD34+ cells have been published, no uniformly accepted guidelines for quantitation of progenitor cells are currently available. Therefore, many centers perform individual protocols for determination of CD34+ cells by different techniques. Preanalytical aspects such as the manner of apheresis or anticoagulation of PBSC products may affect the enumeration of CD34+ cells as well as details in laboratory handling and processing of samples. In particular, the selection and usage of monoclonal antibodies and erythrocyte lysing procedures have a major influence on the determination of CD34+ cells. Accordingly, the first national and international multicenter studies showed large differences in the determination of CD34+ cells. This article reflects the main factors affecting the flow-cytometric determination of CD34+ cells in blood and leukapheresis products as evaluated by own investigations and described in a recently published German consensus protocol. Moreover, alternative techniques for quantitation of CD34+ cells are shortly discussed.

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Kryokonservierung von Blutzellen

Cited by 10 publications

References 55 publications

Processing of peripheral blood progenitor cell components in improved clean areas does not reduce the rate of microbial contamination

Processing of peripheral blood progenitor cell components in improved clean areas does not reduce the rate of microbial contamination

Cryopreservation of Blood

Factors Affecting the Determination of CD34+ Cells in Blood and Leukapheresis Products

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