Kryokonservierung von Humanerythrozyten mit Hydroxyethylstärke (HES) - Teil 2: Vitalitätsanalytik

Sputtek, Andreas; Bacher, Ch.; Langer, Robert; W, Kron; Henrich, H; Rau, G.

doi:10.1159/000222647

Cited by 2 publications

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“…Plasma volume expanders (PVE) that have been developed as plasma substitutes in the treatment of haemorrhage1 or hypovolaemic shock,2 and for cryo‐protection of biological material,3 include proteins such as albumin and gelatin, naturally occurring carbohydrate polymers such as dextran, and chemically modified polysaccharides such as acetyl starch and hydroxyethyl starch (HES). The latter in particular, investigated since 1957, has found widespread acceptance because of the limited adverse reactions4 and the fact that the half‐life time can be carefully controlled based on the average degree of molar substitution 5…”

mentioning

confidence: 99%

Rapid screening of plasma volume expanders in urine using matrix‐assisted laser desorption/ionisation time‐of‐flight mass spectrometry

Gallego

Segura

2004

Rapid Comm Mass Spectrometry

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The use of plasma volume expanders, especially those based on chemically modified polysaccharides such as hydroxyethyl starch, has found its way from the medical field to the athletic community in the everlasting drive for performance enhancement. As such, plasma volume expanders have been placed on the list of banned substances by the International Olympic Committee, and in turn require accurate and sensitive analytical tools for their detection in complex biological matrices. Here we present a relatively straightforward method for the detection of polysaccharide-based plasma volume expanders (PVE) in urine, based on the carefully controlled partial acid hydrolysis of urine (20 microL) in a total volume of 500 microL 4 M trifluoroacetic acid. Following the incubation (30 min at 100 degrees C) an aliquot of the hydrolysate is dried, re-suspended in the analytical matrix (e.g. 2,5-dihydroxybenzoic acid) and examined by matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOFMS). The obtained mass spectrometric profile reveals a high number of characteristic peaks in the mass range between 500 and 3000 Da, a region that in urine samples devoid of PVE appears relatively clean, and thus allows the unambiguous identification of the presence of such PVE. This approach is fast (the mass profile can be obtained within 90 min), highly sensitive (the effective sample amount on the MALDI target is equivalent to 100 nL urine), needs little sample handling (four steps), requires no derivatisation and is devoid of interference from other biomolecules. The approach has been worked-out for hydroxy ethyl starch but can be applied to other polymer-derived plasma expanders such as dextran and probably the newly developed acetyl starch.

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mentioning

confidence: 99%

Rapid screening of plasma volume expanders in urine using matrix‐assisted laser desorption/ionisation time‐of‐flight mass spectrometry

Gallego

Segura

2004

Rapid Comm Mass Spectrometry

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“…Moreover, in the case of hypovolemia, albumin, dextrans, modified gelatins and HES are often transfused to serve as blood volume substitutes. After several in vitro investigations and optimizations of the original HES procedure [14,15] and after in vivo experiments in dogs, Sputtek and coworkers [16] conducted a successful in vivo study including seven healthy volunteers. They subsequently performed a systematic clinical trial in patients [17].…”

Section: Cryopreservation With Hydroxyethyl Starchmentioning

confidence: 99%

Cryopreservation of Erythrocytes, Thrombocytes, and Lymphocytes

Sputtek

Kühnl

Rowe

2007

Transfus Med Hemother

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The cryopreservation of blood cells can be regarded as a classical field of development and application of low temperature biology. Cryopreservation methods have been developed for erythrocytes, which are commonly frozen with glycerol as the cryoprotective additive although hydroxyethyl starch (HES) shows considerable promise. Cryopreserved erythrocytes for transfusion are of advantage in the case of patients with rare blood groups, adverse antibody problems, autologous use and civil as well as military disasters. Additionally they can be used for blood typing, antibody screening and compatibility testing. Cryopreservation methods for thrombocytes, lymphocytes and hematopoietic stem cells usually involve dimethyl sulfoxide (DMSO) as the cryoprotective additive. Low temperature preservation of thrombocytes offers the possibility of making HPA- and/or HLA-typed platelet concentrates available in blood banks at any time. The use of cryopreserved lymphocytes is well established and a routine procedure for clinical laboratory testing. Recently there is a growing clinical interest in cryopreserved lymphocytes in addition to hematopoietic progenitor cells for the supplemental treatment of patients after blood stem cell transplantation. Despite occasional reports, it is our opinion that no clinically suitable method for the preservation of human granulocytes has been developed so far.

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Kryokonservierung von Humanerythrozyten mit Hydroxyethylstärke (HES) - Teil 2: Vitalitätsanalytik

Cited by 2 publications

References 0 publications

Rapid screening of plasma volume expanders in urine using matrix‐assisted laser desorption/ionisation time‐of‐flight mass spectrometry

Rapid screening of plasma volume expanders in urine using matrix‐assisted laser desorption/ionisation time‐of‐flight mass spectrometry

Cryopreservation of Erythrocytes, Thrombocytes, and Lymphocytes

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