KULTIVASI DAN EKSTRAKSI MINYAK DARI MIKROALGA Botryococcus braunii DAN Nannochloropsis sp.

Abstract

Microalgae has high content of vegetable oil. The extraction of microalgae oil can be more efficient with the cell wall destruction, it was aimed to freeing oil that locked inside the cells and be soluble in organic solvent (n-hexane). The study aimed to determined the microalgae growth, the influence of different pH and different species of microalgae with extraction using microwave on oil production by Botryococcus braunii and Nannochloropsis sp. Experimental method with the randomized design was used in this study. The cultivation was performed until the stationery phase is reached so that the biomass can be harvested for oil extraction. The oil compound analysis is conducted using GC-FID. The data in this study has been tested by SPSS 16.0 with t-test and anova two ways. The statistic result showed that there is no differences in cell density of Botryococcus braunii and Nannochloropsis sp. (p= 0,163), likewise with there is no differences in amount of oil extract by Botryococcus braunii and Nannochloropsis sp. (p= 0,323). However, different results are pH treatment influencing oil extraction result (p= 0,006).Composition of fatty acid in Nannochloropsis sp. was dominated by saturated fatty acids, while the fatty acid composition of Botryococcus braunii was equal between saturated an unsaturated fatty acid.
 Keywords: Botryococcus braunii, cultivation, extraction, growth, microalgae, Nannochloropsis sp., oil, pH.

The Lipid Content of The Culture Microalgae Using Media of Tapioca Liquid Waste

“…Chisti (2007) and Chisti (2008), stated that a metabolic relationship between carbohydrates, proteins, and fats, i.e., acetyl-CoA competition, which is a precursor to various biosynthetic pathways such as lipids, proteins, and carbohydrates. Musdalifah et al (2013); Patil et al (2008) and Song et al (2008), stated that microalgae cell lipid biosynthesis is initiated by condensation of glycerol with three fatty acid molecules with the aid of lipase enzyme catalyst. Glycerol derived from α-glycerophosphate removed by phosphate groups by phosphorylation reaction, the formation of fat acids requires some acetyl-CoA, two pairs of electrons (2 NADPH), and ATP energy.…”

Study Literature of Extraction Lipid Method from Microalgae Nannochloropsis sp

“…Extraction research conducted M. Musdalifah et al [11] produced 145.11 grams in 50 liters of media with MAE (Microwave-Assisted Extraction) to optimize the process of breaking down the cell wall to make it easier for oil to get out of the cell and dissolve with n-hexane solvent. The operating conditions at extraction are temperature 75 o C for 10 minutes and wavelength of 2450 MHz.…”

PERTUMBUHAN DAN DAYA SERAP Chlorella vulgaris TERHADAP LOGAM BERAT TIMBAL (Pb) PADA PERAIRAN DALAM SKALA LABORATORIUM

“…Berdasarkan Gambar 1 fase kematian pada perlakuan ini hanya terjadi pada perlakuan kosentrasi logam Pb 9 ppm yang terjadi pada hari ke-8. Perubahan kondisi lingkungan, kekurangan nutrien dan akumulasi racun akibat metabolisme mikroalga menyebabkan mulai terjadinya kematian (Musdalifah et al, 2015). Berdasarkan Gambar 6 C. vulgaris pada hari ke-8 dengan kosentrasti logam berat Pb 3 ppm, 5 ppm 7 ppm masih mengalami fase stasinoer, hal ini dikarenakan C. vulgaris masih mampu mentoleransi Pb.…”