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ABSTRACT Microalgaebiomass containschemicalcomponentssuch as vitamin, proteins, ω-3 and ω-6 polyunsaturated fatty acid and pigments. components with antioxidant activities can be found in only a few species of algae. The potential species of microalgae who have compounds act as antioxidant are Porphyridium cruentum and Chlorella sp. This study aimed to know the growth and antioxidant activity of P. cruentum and Chlorella sp. with the treatment of different cultures. Experimental method and completely randomized design was used as the research method. Antioxidant activity (Ic50 value) was measured by DPPH method (2,2-diphenyl-1-pikrilhidrazil). SPSS 16.0 is used to analyze the data with General Linier Model (GLM) Univariate. The results of GLM Univariate test for cell density showed that there was significant different for cell density in species factors (p= 0,000), but there was no significant different for cell density in culture factors (p= 0,375), and GLM Univariate test for antioxidant activity showed that no significant different for antioxidant activity in species factors (p= 0,522) but siginificant different for antioxidant activity in culture factors (p= 0,001). There was significant different for growth in P. cruentum and Chlorella sp. but no significant different in indoor and outdoor culture, as well as there was no significant different antioxidant activity in two species but significant different in indoor and outdoor culture.   Keywords: growth, antioxidant activity, indoor cultivation, outdoor cultivation.

Microalgae has high content of vegetable oil. The extraction of microalgae oil can be more efficient with the cell wall destruction, it was aimed to freeing oil that locked inside the cells and be soluble in organic solvent (n-hexane). The study aimed to determined the microalgae growth, the influence of different pH and different species of microalgae with extraction using microwave on oil production by Botryococcus braunii and Nannochloropsis sp. Experimental method with the randomized design was used in this study. The cultivation was performed until the stationery phase is reached so that the biomass can be harvested for oil extraction. The oil compound analysis is conducted using GC-FID. The data in this study has been tested by SPSS 16.0 with t-test and anova two ways. The statistic result showed that there is no differences in cell density of Botryococcus braunii and Nannochloropsis sp. (p= 0,163), likewise with there is no differences in amount of oil extract by Botryococcus braunii and Nannochloropsis sp. (p= 0,323). However, different results are pH treatment influencing oil extraction result (p= 0,006).Composition of fatty acid in Nannochloropsis sp. was dominated by saturated fatty acids, while the fatty acid composition of Botryococcus braunii was equal between saturated an unsaturated fatty acid. Keywords: Botryococcus braunii, cultivation, extraction, growth, microalgae, Nannochloropsis sp., oil, pH.

AbstrakMikro alga jenis Chlorophyceae SP adalah alga laut yang menghasilkan zat gizi seperti asam lemak tak jenuh omega-3, 6, dan 9, serat, vitamin, protein dan mineral. Warna hijau dari klorofil pada Chlorella,sp disebut darah hijau (green blood) mempunyai kandungan zat besi pembentuk hemoglobin. Pada Chlorella,sp kering terdapat enzim Superoxide dismutase (SOD) sekitar 10.000-37.500 units per 10 gram yang merupakan anti radikal bebas untuk mencegah penuaan dini. Sementara produksi Chorophyceae secara alami masih terbatas, Untuk itu ada teknik budidaya alga dengan memanipulasi media hidup yaitu media sintetik. Penelitian ini adalah penelitian pendahuluan melihat pengaruh perlakuan media tumbuh terhadap pertumbuhan atau kepadatan sel dengan menggunakan Hemaesitometry. Pupuk anorganik proanalis dan soil extract ditambahkan ke dalam wadah kultivasi. Chlorella, sp pada umur kultivasi 13 hari menghasilkan jumlah sel tertinggi sebanyak 7,7567 log sel/mL (5,7120x10 7 sel/mL. Chlorella, sp pada pupuk soil extract pada umur kultivasi 9 hari menghasilkan jumlah sel tertinggi sebanyak 5,7533 log sel/mL (5,6666x10 7 sel/mL). Laju pertumbuhan Chlorella,sp menunjukkan hasil paling baik pada media kultivasi yang menggunakan pupuk anorganik (conwy) dibandingkan dengan pupuk soil extract.Kata Kunci : Chlorella, sp, SOD, pupuk anorganik, soil extrac. AbstractChlorella, sp is a kind of algae. Naturally Chlorella, sp life in the sea a limited amount. They have produced the nutritions : omega-3,6 and 9 acid fat,vitamine, protein, mineral and chlorofil. It also contain an Antioxidant enzyme such as Superoxide dismutase (SOD). This enzyme have an ability as radicals scavenging. The enzyme activity of SOD is 10.000-37.500 units /10 g of wet weight. The present primery research by doing modification of media as habitate of Chlorella, sp.,. Anorganic and soil extract fertilizer were added to 10 4 cell/ml Chlorella, sp. and then the density of Chlorella, sp was measured by Haemacytometer. It was found that the highest cell of Chlorella, sp showed after 13 days cultivification was 7,7567 log cells/mL(5,7120x10 7 cellsl/mL on anorganic media. Chlorella, sp on soil extract were found the highest cells was 5,7533 log celsl/mL (5,6666x10 7 cells/mL) after 9 days cultivication r. The growth of Chlorella,sp was found to be the best on media cultivivication using anorganic (conwy) fertilizer.

AbstrakSalah satu dampak kekurangan asam-asam lemak omega-3 dan omega-6 di Indonesia adalah rendahnya tingkat kesehatan ibu hamil. Pemberian pelengkap nutrisi yang mengandung DHA, EPA dan AA dapat menjadi solusi permasalahan tersebut. Pada umumnya suplemen tersebut diperoleh dari minyak ikan laut, namun sumber ini memiliki beberapa kekurangan yang mempengaruhi kualitas asam lemak yang dihasilkan, sehingga diperlukan pengganti minyak ikan laut sebagai sumber utama DHA, EPA dan AA. Salah satu sumber pelengkap nutrisi yang sangat potensial adalah mikroalga yang dikultivasi secara heterotrof. Pada penelitian ini dilakukan kultivasi mikroalga spesies Spirulina platensis dan Botyrococcus braunii yang dikoleksi oleh Balai Besar Bioteknologi dan Perikanan di Jakarta. Kultivasi masing-masing mikroalga tersebut mula-mula dilakukan secara normal (autotrof), kemudian dikondisikan menjadi heterotrof dengan pemberian glukosa 0,5 g/L. Hasil ekstraksi dengan gelombang mikro menunjukkan bahwa S. platensis dan B. braunii yang dikultivasi secara heterotrof memiliki kandungan minyak berturut-turut sebesar 5,297 dan 0,173 %-b (berdasarkan biomassa kering). Kandungan DHA, EPA, dan AA dalam minyak dari kedua mikroalga tersebut adalah berturut-turut sebesar 0,006, 0,002, dan 0,001 %-massa relatif terhadap minyak. Abstract PRODUCTION OF NUTRITIONAL SUPPLEMENTS FROM SPIRULINA PLATENSIS AND BOTYROCOCCUS BRAUNII MARINE MICROALGAE.The low level of health of pregnant women is one of the results from the deficiency of omega-3 and omega-6 fatty acids in Indonesia. Giving supplement containing DHA, EPA, and AA, can solve the problem. Usually, these nutritional supplements are produced from marine fish oil. However, this source has several deficiencies that influence the quality of the fatty acid produced. Therefore, alternative sources of oil need to be found that can replace fish oil to produce DHA, EPA, and AA. One of the very potential sources is heterotrophic cultivated microalgae. In this study, microalgae from species Spirulina platensis and Botyrococcus braunii collected from Research Center for Marine and Fisheries Product Processing and Biotechnology in Jakarta, were cultivated. Once microalgae is cultivated autotrophically, the culture were transformed to heterotrophic condition by adding 0.5 g/L glucose solution. Results showed that lipid yield from S.platensis and B. braunii were 5.297 and 0.173 (%-w dry biomass), respectively. Composition of DHA, EPA and AA from S.platensis and B.braunii oils were 0.006, 0.002, and 0.001 (%-w of lipid), respectively.