L-17 family cytokines in neutrophils of patients with oral epithelial squamous cell carcinoma

Abstract

biobran, enzymatically modified arabinoxylan from rice bran was tested for its possible effects on in vitro maturation of human dendritic cells (DC). immature DC (iDC) derived from plastic-adhered, il-4 and GM-CSF treated peripheral monocytes (Mo) were further cultured with cytokine maturation mix 1 (CMM1; TNF-α, il-1β and il-6) or CMM2 (lPS and iFN-γ) to induce their maturation into mature DC (matDC1 or matDC2, respectively). Different concentrations of biobran (10, 100, 400 and 1000 µg/ml) were applied in the presence or absence of relevant CMM to assess the effects of biobran on DC maturation processes. biobran induced maturation of iDC, as these cells cultured with il-4/GM-CSF/biobran downregulated CD14 and CD1a antigens on cell surface and significantly increased expression of maturation marker CD83. The increase of surface density of costimulatory molecules CD80 and CD86 on iDC in the presence of biobran was also observed. in addition, biobran induced functional maturation of iDC, confirmed by decreased endocytic activity of iDC. Furtheremore, biobran enhanced maturation potential of cytokine mixes, as both matDC1 and matDC2 exposed to biobran completely lost CD14 and upregulated CD83, CD80 and CD86 antigens, in comparison to DC matured with the relevant CMM alone. biobran also increased CD123 antigen expression on all DC subsets. interestingly, matDC2 matured in the presence of biobran (400µg/ml) expressed higher levels of CD123 and lower levels of CD11c cell surface antigens, the phenotype represented by CD11c dim CD123 bright plasmacytoid DC population. These data demonstrate that biobran is a potent enhancer of DC maturation and suggest that biobran might be a useful agent to create the environment that favours DC maturation.

IL-17A as a regulator of neutrophil survival in nasal polyp disease of patients with and without cystic fibrosis

“…Moreover, neutrophils isolated from the blood of non-CF and CF-patients gave comparable results: IL-17A could decrease the apoptosis in non-CF patients but not in CF patients. Recently Pelletier et al [28] discussed that neutrophils lack the IL-17R receptor and consequently IL-17A could not directly activate neutrophils; however, others proved the presence of IL-17R on neutrophils [29] and Th17 cells could also activate neutrophils through the release of GM-SCF, interferon-γ or TNF-α [16,30]. In the tissue single cell suspension from non-CF patients we found a clear effect on the survival of neutrophils after adding IL-17A, a Th17 cell product.…”

Interleukin-25 induces type 2 cytokine production in a steroid-resistant interleukin-17RB+ myeloid population that exacerbates asthmatic pathology

“…Because IL-17RB + subsets could be distinguished based upon the intensity of Gr-1 expression, and in light of the differing capacity for IL-4 and IL-13 production between Gr-1 mid and Gr-1 hi populations, IL-17RB + Gr-1 hi cells may have functions that overlap with other CD11b + Gr-1 + populations, such as myeloid suppressor cells 39,40 . A recent study reported that both IL-17RA and IL-17RB can be expressed on the surface of human neutrophils 41 . Clinical studies of patients with steroid resistant asthma demonstrated that a neutrophilic inflammatory response is predominant, while animal studies have suggested that steroid resistant Th17 cells may explain neutrophil-mediated responses 42–50 .…”

Interleukin-17 may be a valuable serum tumor marker in patients with colorectal carcinoma

“…The role of metalloproteinases in the progression of human malignancies has been well documented in numerous reports [63,64]. Likewise, some authors have reported new aspects of the biological role of IL-17 family cytokines, as well as indicators of leukocyte activity in IL-17A and IL-17E-dependent reactions in patients with oral epithelial squamous cell carcinoma [65].…”