l-Arginine Improves Wound Healing after Trauma-Hemorrhage by Increasing Collagen Synthesis

Wittmann, F; Prix, Niclas J.; Mayr, Stefan; Angele, Peter; Wichmann, M. W.; Engel, Nicole; Hernandez-Richter, T.; Chaudry, Irshad H.; Jauch, Karl Walter; Angele, Martin K.

doi:10.1097/01.ta.0000171529.06625.a8

Cited by 31 publications

(19 citation statements)

References 26 publications

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“…The current study confirms and expands prior studies of Wittmann et al 18 In that murine model, OHP was significantly decreased following trauma/hemorrhage; L-arginine increased maximal WBS and restored depressed OHP levels. Our study uses a more severe injury model and confirms the benefit of including arginine as part of postresuscitative therapy.…”

Section: Discussionsupporting

confidence: 91%

“…This effect was noted in both normal and T/HS rats. The mechanistic explanations for these phenomena are complex, and may be mediated through l ‐arginine's stimulatory immunologic and endocrine secretagogic effects (particularly through the induction of the growth hormone/IGF‐1 pathway) 1,18 . Likewise, NO, the direct product of l ‐arginine catabolism by iNOS, has been shown to regulate directly various transcription factors, including nuclear factor–κ‐B (NF‐κB) 27 …”

Section: Discussionmentioning

confidence: 99%

“…The mechanistic explanations for these phenomena are complex, and may be mediated through L-arginine's stimulatory immunologic and endocrine secretagogic effects (particularly through the induction of the growth hormone/IGF-1 pathway). 1,18 Likewise, NO, the direct product of L-arginine catabolism by iNOS, has been shown to regulate directly various transcription factors, including nuclear factor-k-B (NF-kB). 27 In summary, our data indicate that L-arginine supplementation improves normal and trauma/hemorrhageimpaired wound healing.…”

Section: Discussionmentioning

confidence: 99%

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Supplemental l‐arginine enhances wound healing following trauma/hemorrhagic shock

Shi

Wang

Zhang³

et al. 2007

Wound Repair Regeneration

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To determine whether parenteral L-arginine supplementation enhances the impaired wound healing of rats subjected to trauma/hemorrhagic shock. Impaired wound healing after trauma and shock has been documented experimentally and clinically. L-arginine has been shown to enhance wound strength and collagen synthesis in rodents and humans. Its efficacy under conditions of impaired wound healing is less well defined. Forty-eight male Lewis rats were used in this study. Using a well-defined model, 24 rats underwent trauma/hemorrhagic shock before wounding. Twenty-four untreated rats served as controls. All animals underwent a dorsal skin incision with implantation of polyvinyl-alcohol sponges. Half of the animals in each group were assigned to receive 1 g/kg/day of L-arginine by intraperitoneal injection in three divided doses, while the other half received saline injections only. Animals were sacrificed 10 days postwounding, and wound-breaking strength (WBS) and wound sponge total hydroxyproline (OHP) and nitrite/nitrate (NO(x)) content were determined. Wound sponge RNA was collected and subjected to Northern blot analysis for procollagens I and III. Trauma/hemorrhage greatly decreased WBS with a concomitant diminution in collagen (OHP) deposition. L-arginine significantly enhanced WBS (19%) and increased OHP (21%) levels in control animals as well as in rats subjected to trauma/hemorrhage (WBS +29%, OHP 40%) compared with their saline-treated counterparts. Procollagen I and III mRNA levels were elevated by L-arginine treatment in both trauma/hemorrhage and control rats. Arginine treatment had no effect on wound fluid and plasma NO(x). The data demonstrate that the impaired healing subsequent to trauma/hemorrhage can be greatly alleviated by L-arginine supplementation.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Supplemental l‐arginine enhances wound healing following trauma/hemorrhagic shock

Shi

Wang

Zhang³

et al. 2007

Wound Repair Regeneration

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“…This depletes l -arginine from the micro-environment. The amino acid l -arginine has multiple roles such as its importance in wound healing [73]. In addition, it is the only endogenous substrate for the production of nitric oxide (NO) by inducible nitric oxide synthase (iNOS) [74].…”

Section: Mechanisms Of Immune Suppression By Suppressive Neutrophils mentioning

confidence: 99%

Immune suppression by neutrophils and granulocytic myeloid-derived suppressor cells: similarities and differences

Pillay

Tak

Kamp

et al. 2013

Cell. Mol. Life Sci.

333

339

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Neutrophils are essential effector cells in the host defense against invading pathogens. Recently, novel neutrophil functions have emerged in addition to their classical anti-microbial role. One of these functions is the suppression of T cell responses. In this respect, neutrophils share similarities with granulocytic myeloid-derived suppressor cells (G-MDSCs). In this review, we will discuss the similarities and differences between neutrophils and G-MDSCs. Various types of G-MDSCs have been described, ranging from immature to mature cells shaping the immune response by different immune suppressive mechanisms. However, all types of G-MDSCs share distinct features of neutrophils, such as surface markers and morphology. We propose that G-MDSCs are heterogeneous and represent novel phenotypes of neutrophils, capable of suppressing the immune response. In this review, we will attempt to clarify the differences and similarities between neutrophils and G-MDSCs and attempt to facilitate further research.

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“…Humans produce enough L‐Arg to sustain normal collagen turnover but not in the case of injury when massive collagen synthesis is required. Diet supplementation with L‐Arg has been shown to improve wound healing after trauma and haemorrhage by increasing collagen synthesis (Wittmann et al , ). L‐Arg also increases collagen synthesis in osteoblast‐like cells (Chevalley et al , ).…”

Section: Introductionmentioning

confidence: 99%

Lysophosphatidic acid enhances collagen deposition and matrix thickening in engineered tissue

Chabaud

Marcoux

Deschênes-Rompré

et al. 2013

J Tissue Eng Regen Med

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The time needed to produce engineered tissue is critical. A self-assembly approach provided excellent results regarding biological functions and cell differentiation because it closely respected the microenvironment of cells. Nevertheless, the technique was time consuming for producing tissue equivalents with enough extracellular matrix to allow manipulations. Unlike L-arginine supplementation that only increased accumulation of collagen in cell culture supernatant in our model, addition of lysophosphatidic acid, a natural bioactive lipid, did not modify the amount of accumulated collagen in the cell culture supernatant; however, it enhanced the matrix deposition rate without inducing fibroblast hyperproliferation and tissue fibrosis.

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l-Arginine Improves Wound Healing after Trauma-Hemorrhage by Increasing Collagen Synthesis

Abstract: Because L-arginine improves wound healing, the results suggest that L-arginine might represent a novel and useful adjunct to fluid resuscitation for decreasing wound complications after trauma and severe blood loss.

Cited by 31 publications

References 26 publications

Supplemental l‐arginine enhances wound healing following trauma/hemorrhagic shock

Supplemental l‐arginine enhances wound healing following trauma/hemorrhagic shock

Immune suppression by neutrophils and granulocytic myeloid-derived suppressor cells: similarities and differences

Lysophosphatidic acid enhances collagen deposition and matrix thickening in engineered tissue

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