L-carnitine significantly decreased aging of rat adipose tissue-derived mesenchymal stem cells

Abstract

Mesenchymal stem cells are undifferentiated cells that have the ability to divide continuously and tissue regeneration potential during the transplantation. Aging and loss of cell survival, is one of the main problems in cell therapy. Since the production of free radicals in the aging process is effective, the use of antioxidant compounds can help in scavenging free radicals and prevent the aging of cells. The aim of this study is evaluate the effects of L-carnitine (LC) on proliferation and aging of rat adipose tissue-derived mesenchymal stem cells (rADSC). rADSCs were isolated from inguinal region of 5 male Rattus rats. Oil red-O, alizarin red-S and toluidine blue staining were performed to evaluate the adipogenic, osteogenic and chondrogenic differentiation of rADSCs, respectively. Flow cytometric analysis was done for investigating the cell surface markers. The methyl thiazol tetrazolium (MTT) method was used to determine the cell proliferation of rADSCs following exposure to different concentrations of LC. rADSCs aging was evaluated by beta-galactosidase staining. The results showed significant proliferation of rADSCs 48 h after treatment with concentrations of 0.2 mM LC. In addition, in the presence of 0.2 mM LC, rADSCs appeared to be growing faster than control group and 0.2 mM LC supplementation could significantly decrease the population doubling time and aging of rADSCs. It seems that LC would be a good antioxidant to improve lifespan of rADSCs due to the decrease in aging.

Curcumin Affects Adipose Tissue-Derived Mesenchymal Stem Cell Aging Through TERT Gene Expression

“…After PCR was done, amplified PCR products were analyzed by ethidium bromide staining after 1.5 % agarose gel electrophoresis. Primer sequences are shown in ▶table 1 [18].…”

“…To determine the PDN, the following formula was used: PDN = log (N1/N0) × 3.31. In this equation, N1 is the cell number at the end of cultivation period and N0 is the cell number at culture initiation [18].…”

“…After the incubation, cells were wash twice with PBS, and once with methanol and the dish was allowed to air dry. A minimum of 100 cells were counted by light microscopy in 10 random fields to determine the percentage of SA-ß-gal-positive cells which appeared as blue-stained cells [18].…”

Rag1 immunodeficiency‐induced early aging and senescence in zebrafish are dependent on chronic inflammation and oxidative stress

“…ABT‐263 is a potent inhibitor of the Bcl‐2 family of anti‐apoptotic factors and is a promising chemotherapeutic drug for cancer cell treatment (Oltersdorf et al, ; Tse et al, ), but currently, ABT‐263 is considered a highly selective senolytic agent (Chang et al, ; Pan et al, , 62). In addition, in vitro L‐carnitine or ALCAR administration has been proven to prevent senescent cell formation during aging due to the antioxidant activity of these compounds (Mobarak, Fathi, Farahzadi, Zarghami, & Javanmardi, ; Wu et al, ). ABT‐263 reduced tp53 , mdm2, and p21 expression in wt, but this effect was extended to p27 , p57 , cdkn2a/b, and cdkn2c in rag1 −/− zebrafish.…”

TRIB3 inhibits proliferation and promotes osteogenesis in hBMSCs by regulating the ERK1/2 signaling pathway

“…Cell osteogenically induced for 0, 3, 7 and 14 days were stained with ARS to investigate the matrix concentrations of calcium and phosphate. ARS was performed according to previously described procedures 63 . Cells were washed thrice with PBS and fixed in ice-cold 95% ethanol for 10 min.…”