L-Cysteine as an Irreversible Inhibitor of the Peroxidase-Mimic Catalytic Activity of 2-Dimensional Ni-Based Nanozymes

Liyanage, Piyumi Dinusha; Weerathunge, Pabudi; Singh, Mandeep; Bansal, Vipul; Ramanathan, Rajesh

doi:10.3390/nano11051285

Cited by 23 publications

(16 citation statements)

References 57 publications

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“…It has been reported that L‐cysteine can inhibit the enzyme‐like activity of nanomaterials through the interaction of −SH with metal active centers, thus resulting in changes of oxTMB absorbance [44] . Based on this unique mechanism, diverse metal‐based POD‐like nanomaterials have been designed to detect L‐cysteine [44,45] . Due to the excellent POD‐like activity of Cu‐PcCP NPs, the catalytic sensing of L‐cysteine is carefully investigated via TMB mediated POD‐like activity assay (Figure 7a).…”

Section: Resultsmentioning

confidence: 99%

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π‐Conjugated Copper Phthalocyanine Nanoparticles as Highly Sensitive Sensor for Colorimetric Detection of Biomarkers

Chen

Zhu

et al. 2022

Chemistry A European J

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Though numerous nanomaterials with enzyme-like activities have been utilized as probes and sensors for detecting biological molecules, it is still challenging to construct highly sensitive detectors for biomarkers using polymeric materials. Benefiting from the π-d delocalization effect of electrons, excellent metal-chelating property, high electron transferability, and good chemical stability of πconjugated phthalocyanine, the design of the copper phthalocyanine-based conjugated polymer nanoparticles (Cu-PcCP NPs) as a colorimetric sensor for a variety of biomarkers is reported. The Cu-PcCP NPs are synthesized through a simple microwave-assisted polymerization, and their chemical structures are thoroughly characterized. The colorimetric results of Cu-PcCP NPs demonstrate excellent peroxidase-like detecting activity and also great substrate selectivity than most of the reported Cu-based nanomaterials. The Cu-PcCP NPs can achieve a detection limit of 4.88 μM for the H 2 O 2 , 4.27 μM for the L-cysteine, and 21.10 μM for the glucose via a cascade catalytic system, which shows comparable detecting sensitivity as that of many earlier reported enzyme-like nanomaterials. Moreover, Cu-PcCP NPs present remarkable resistance to harsh conditions, including high temperature, low pH, and excessive salts. These highly specific π-conjugated copper-phthalocyanine nanoparticles not only overcome the current limitation of polymeric material-based sensors but also provide a new direction for designing next-generation enzyme-like nanomaterial-based colorimetric biosensors.

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Section: Resultsmentioning

confidence: 99%

“…[44] Based on this unique mechanism, diverse metal-based POD-like nanomaterials have been designed to detect L-cysteine. [44,45] Due to the excellent…”

Section: Colorimetric Detection Of L-cysteinementioning

confidence: 99%

π‐Conjugated Copper Phthalocyanine Nanoparticles as Highly Sensitive Sensor for Colorimetric Detection of Biomarkers

Chen

Zhu

et al. 2022

Chemistry A European J

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“…Following the in-depth characterization of the Co 3 S 4 nanosheets, we first explored their ability to mimic the catalytic activity of the natural peroxidase enzyme. ,,,− For this, the ability of Co 3 S 4 nanosheets to convert the colorless diamine TMB to a blue colored charge transfer product (λ max at ca. 650 nm) was assessed following its incubation with peroxidase substrates TMB and H 2 O 2 . , As shown in Figure a, the Co 3 S 4 nanosheets could rapidly oxidize TMB in the presence of H 2 O 2 at initial time points with the activity saturating within 15 min of the reaction.…”

Section: Results and Discussionmentioning

confidence: 99%

“…This reversible binding showed similarity to the enzymatic competitive inhibition process. Subsequently, our more recent work has shown that l -cysteine binds irreversibly to Ni-based NZs . This difference in the mechanism suggested that the composition of the NZ can have a major role in the ability of the NZ to interact with l -cysteine.…”

Section: Introductionmentioning

confidence: 99%

“…In the context of catalysis, enzyme-mimicking catalytic nanoparticles or nanozymes (NZs) have gained remarkable interest with wide-spread applicability across sensing, bioimaging, environmental clean-up, and pro-drug therapy. − Given that the composition of the nanoparticle can influence the catalytic efficiency, − a suite of nanoparticles including metals, metal oxides, metal sulfides, etc ., have been reported for wide-ranging enzyme-mimic activities. , Of these, metal sulfides have been suggested to be superior catalyst candidates as the M–S bonds are weaker than the M–O bonds in the corresponding metal oxides . Further, NZ-based sensors have gained attention in analytical science as they allow visual detection of the sensing event. , Such sensors have been used for the detection of several chemical and biological molecules including l -cysteine, a thiol containing semi-essential amino acid found in a number of proteins. , The detection of l -cysteine is important as its deficiency can cause anomalous cell growth, skin lesions, lethargy, hair depigmentation, liver damage, and malabsorption syndrome, while high levels of l -cysteine can be neurotoxic and cause cardiovascular diseases …”

Section: Introductionmentioning

confidence: 99%

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Cobalt Sulfide Nanosheets as Peroxidase Mimics for Colorimetric Detection of l-Cysteine

Hashmi

Singh

Weerathunge

et al. 2021

ACS Appl. Nano Mater.

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The rich chemical, physical, electronic, and surface properties of two-dimensional (2D) materials have witnessed an explosion of research leading to enrichment in the 2D family of materials. Cobalt sulfide (Co x S y ), a transition metal chalcogenide, has attracted significant interest as its different stoichiometric and non-stoichiometric phases offer diverse properties. To date, the Co3S4 phase has seen limited developments with recent reports suggesting good catalytic properties of this material. In the current study, we show the facile synthesis of phase pure 2D Co3S4 nanosheets using a hydrothermal approach and demonstrate the importance of the chemical reactivity of the surface atoms in achieving target selectivity during sensing. First, the catalytic activity of Co3S4 nanosheets was observed to follow Michaelis–Menten kinetics, suggesting that the nanosheets mimic the catalytic activity of the natural peroxidase enzymes. The presence of dangling sulfur atoms on the surface of the Co3S4 nanosheets allowed them to specifically interact with l-cysteine amino acid that resulted in the temporary loss of its catalytic activity. This interaction allowed us to develop a “turn-off” colorimetric sensor to detect l-cysteine even in complex mixtures of amino acids and other sulfur containing compounds. In-depth understanding of the sensor mechanism revealed a reversible competition between l-cysteine and peroxidase substrates for binding to the Co3S4 nanosheets. Due to the higher affinity of l-cysteine to the nanosheets, it first binds to the Co3S4 nanosheets blocking its nanozyme activity. Subsequently, the outstanding catalytic activity of 2D Co3S4 nanosheets assists in the oxidation of l-cysteine, and as oxidized products leave the nanozyme surface, its catalytic activity resumes. The findings of our study are likely to instigate further research into the exploration of the chemical reactivity of 2D surfaces for diverse applications.

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Silver-based bimetallic nanozyme fabrics with peroxidase-mimic activity for urinary glucose detection

Naveen Prasad,

Mahasivam,

Ramanathan

et al. 2024

Anal Bioanal Chem

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The enhanced catalytic properties of bimetallic nanoparticles have been extensively investigated. In this study, bimetallic Ag-M (M = Au, Pt, or Pd) cotton fabrics were fabricated using a combination of electroless deposition and galvanic replacement reactions, and improvement in their peroxidase-mimicking catalytic activity compared to that of the parent Ag fabric was studied. The Ag-Pt bimetallic nanozyme fabric, which showed the highest catalytic activity and ability to simultaneously generate hydroxyl (•OH) and superoxide (O2•−) radicals, was assessed as a urine glucose sensor. This nanozyme fabric sensor could directly detect urinary glucose in the pathophysiologically relevant high millimolar range without requiring sample predilution. The sensor could achieve performance on par with that of the current clinical gold standard assay. These features of the Ag-Pt nanozyme sensor, particularly its ability to avoid interference effects from complex urinary matrices, position it as a viable candidate for point-of-care urinary glucose monitoring. Graphical Abstract

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L-Cysteine as an Irreversible Inhibitor of the Peroxidase-Mimic Catalytic Activity of 2-Dimensional Ni-Based Nanozymes

Cited by 23 publications

References 57 publications

π‐Conjugated Copper Phthalocyanine Nanoparticles as Highly Sensitive Sensor for Colorimetric Detection of Biomarkers

π‐Conjugated Copper Phthalocyanine Nanoparticles as Highly Sensitive Sensor for Colorimetric Detection of Biomarkers

Cobalt Sulfide Nanosheets as Peroxidase Mimics for Colorimetric Detection of l-Cysteine

Silver-based bimetallic nanozyme fabrics with peroxidase-mimic activity for urinary glucose detection

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