L4 stage Heligmosomoides polygyrus prevents the maturation of dendritic JAWS II cells

Maruszewska-Cheruiyot, Marta; Donskow‐Łysoniewska, Katarzyna; Piechna, Karolina; Krawczak, Katarzyna; Doligalska, Maria

doi:10.1016/j.exppara.2018.10.010

Cited by 9 publications

(3 citation statements)

References 49 publications

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“…The mice were euthanized six days after infection, when the parasite was in its fourth larval stage. The larvae were collected and the sex of the L4 stage was determined as described before [ 53 ]. Two separate experiments were carried out on ES generated from nematode cultures collected from independently infected mice.…”

Section: Methodsmentioning

confidence: 99%

The Impact of Intestinal Inflammation on Nematode’s Excretory–Secretory Proteome

Maruszewska-Cheruiyot,

Szewczak,

Krawczak-Wójcik

et al. 2023

IJMS

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Parasitic nematodes and their products are promising candidates for therapeutics against inflammatory bowel diseases (IBD). Two species of nematodes, the hookworm Necator americanus and the whipworm Trichuis suis, are being used in clinical treatment trials of IBD referred to as “helminth therapy”. Heligmosomoides polygyrus is a well-known model for human hookworm infections. Excretory–secretory (ES) products of H. polygyrus L4 stage that developed during colitis show a different immunomodulatory effect compared to the ES of H. polgyrus from healthy mice. The aim of the study was to evaluate excretory–secretory proteins produced by H. polygyrus L4 stage males and females that developed in the colitic milieu. Mass spectrometry was used to identify proteins. Blast2GO was used to investigate the functions of the discovered proteins. A total of 387 proteins were identified in the ES of H. polygyrus L4 males (HpC males), and 330 proteins were identified in the ES of L4 females that developed in the colitic milieu (HpC females). In contrast, only 200 proteins were identified in the ES of L4 males (Hp males) and 218 in the ES of L4 females (Hp females) that developed in control conditions. Most of the proteins (123) were detected in all groups. Unique proteins identified in the ES of HpC females included annexin, lysozyme-2, apyrase, and galectin. Venom allergen/Ancylostoma-secreted protein-like, transthyretin-like family proteins, and galectins were found in the secretome of HpC males but not in the secretome of control males. These molecules may be responsible for the therapeutic effects of nematodes in DSS-induced colitis.

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Section: Methodsmentioning

confidence: 99%

The Impact of Intestinal Inflammation on Nematode’s Excretory–Secretory Proteome

Maruszewska-Cheruiyot,

Szewczak,

Krawczak-Wójcik

et al. 2023

IJMS

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“…Prevention of DC maturation, upon incubation with L4 larvae of Heligmosomoides polygyrus [ 128 ] and the ability of adult stage H. polygyrus somatic antigens to inhibit proliferation and apoptosis of host immune cells [ 129 ] are other examples where parasite regulation of the NF-κB pathway is utilized by nematodes to modulate host immune responses and facilitate survival.…”

Section: Helminthsmentioning

confidence: 99%

The Role of Nuclear Factor Kappa B (NF-κB) in the Immune Response against Parasites

Bąska

Norbury

2022

Pathogens

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The immune system consists of various cells, organs, and processes that interact in a sophisticated manner to defend against pathogens. Upon initial exposure to an invader, nonspecific mechanisms are raised through the activation of macrophages, monocytes, basophils, mast cells, eosinophils, innate lymphoid cells, or natural killer cells. During the course of an infection, more specific responses develop (adaptive immune responses) whose hallmarks include the expansion of B and T cells that specifically recognize foreign antigens. Cell to cell communication takes place through physical interactions as well as through the release of mediators (cytokines, chemokines) that modify cell activity and control and regulate the immune response. One regulator of cell states is the transcription factor Nuclear Factor kappa B (NF-κB) which mediates responses to various stimuli and is involved in a variety of processes (cell cycle, development, apoptosis, carcinogenesis, innate and adaptive immune responses). It consists of two protein classes with NF-κB1 (p105/50) and NF-κB2 (p100/52) belonging to class I, and RelA (p65), RelB and c-Rel belonging to class II. The active transcription factor consists of a dimer, usually comprised of both class I and class II proteins conjugated to Inhibitor of κB (IκB). Through various stimuli, IκB is phosphorylated and detached, allowing dimer migration to the nucleus and binding of DNA. NF-κB is crucial in regulating the immune response and maintaining a balance between suppression, effective response, and immunopathologies. Parasites are a diverse group of organisms comprised of three major groups: protozoa, helminths, and ectoparasites. Each group induces distinct effector immune mechanisms and is susceptible to different types of immune responses (Th1, Th2, Th17). This review describes the role of NF-κB and its activity during parasite infections and its contribution to inducing protective responses or immunopathologies.

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“…Using murine cell lines is very useful due to high cell yields which facilitate experiments and allow repeated measurements. JAWS II cells are being used successfully in DC research on cancer vaccine delivery (1), anti-tumour effect (2), immunoparasitological study (3,4) and others (5)(6)(7)(8)(9)(10)(11). Immunotherapeutic vaccines show a promising weapon in the fight against cancer, especially when a personalized approach to the patient's disease is required.…”

Section: Introductionmentioning

confidence: 99%

Heterogeneity of adherent and non‑adherent JAWS II dendritic cells

Maruszewska-Cheruiyot

Machcińska

Kierasińska

et al. 2023

Oncol Lett

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Dendritic cells (DCs) are crucial in the development of immune responses. DC JAWS II is a murine cell line frequently used in DC studies. These cells are grown in two cell fractions: Adherent and non-adherent. The present study aimed to compare these two fractions in both immature and lipopolysaccharide (LPS)-activated JAWS II cells. The present study analysed the condition, phenotype, antigen uptake capability, signalling properties and the influence on the activity of T cells using flow cytometry, mixed cell reaction and ELISA methods. Adherent immature JAWS II cells exhibited increased endocytosis and decreased activation of the Pi3K signalling pathway. After LPS activation, adherent JAWS II cells exhibited increased expression levels of CD80 and CD86 costimulatory molecules, increased endocytosis and an elevated ability to induce T cell proliferation, compared with non-adherent cells. These results demonstrated that the two fractions of JAWS II adherent and non-adherent cells exhibited different properties and this should be taken into account in the planning of research.

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L4 stage Heligmosomoides polygyrus prevents the maturation of dendritic JAWS II cells

Cited by 9 publications

References 49 publications

The Impact of Intestinal Inflammation on Nematode’s Excretory–Secretory Proteome

The Impact of Intestinal Inflammation on Nematode’s Excretory–Secretory Proteome

The Role of Nuclear Factor Kappa B (NF-κB) in the Immune Response against Parasites

Heterogeneity of adherent and non‑adherent JAWS II dendritic cells

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