Lab-In-Syringe for automated double-stage sample preparation by coupling salting out liquid-liquid extraction with online solid-phase extraction and liquid chromatographic separation for sulfonamide antibiotics from urine

Fikarová, Kateřina; Horstkotte, Burkhard; Machián, Daniel; Sklenářová, Hana; Solich, Petr

doi:10.1016/j.talanta.2020.121427

Cited by 43 publications

(18 citation statements)

References 36 publications

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“…It is therefore logical that there is a growing demand for more targeted and personalized antibiotic dosing. Currently, a number of methods for the determination of antibiotics in various matrices are available, including high-performance liquid chromatography (HPLC) [2][3][4][5][6][7][8] or mass spectrometry [9,10], or a combination of HPLC with mass spectrometry [5,[11][12][13] or with electrochemical methods [14][15][16]. However, number of articles dealing with the determination of ampicillin is limited.…”

Section: Introductionmentioning

confidence: 99%

“…Analyses with the use of the column have not yet been published. The new stationary phase was compared with other polar-endcapped stationary phase Luna ® Omega Polar C18 that has been on the market for a long time and has already been used for the determination of other antibiotics in biological samples [8]. For these reasons, this column was chosen as reference.…”

Section: Introductionmentioning

confidence: 99%

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Separation of ampicillin on polar-endcapped phase: Development of the HPLC method to achieve its correct dosage in cardiac surgery

Kubíčková

Rácová

Strojil

et al. 2023

AChrom

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The accurate, simple, and selective reversed phase high performance liquid chromatography (RP-HPLC) has been established and validated for the determination of an antibiotic ampicillin (AMP) in human blood plasma. The SPE extraction was used for the sample preparation. Chromatographic separation was accomplished by a mobile phase containing 15 mM monopotassium phosphate solution of pH 3.3 and methanol (75:25, v/v) in an isocratic mode at a flow rate of 1.4 mL min−1 at 30 °C. The separation was evaluated on a column with a new polar-endcapped C18 stationary phase Arion® Polar C18 or well-known phase Luna® Omega Polar C18. Excellent linearity (R 2 0.9998) was shown over range 10–300 mg L−1 with mean percentage recovery 90%. Peak shapes were symmetrical in both columns, Arion® Polar C18 and Luna® Omega Polar C18, with asymmetry factor of 1.0 and 1.4, tailing factor of 1.0 and 1.2, and retention factor of 4.6 and 5.6, respectively. The Arion® Polar C18 was almost 1.4-fold more effective than Luna® Omega Polar C18 phase. The LOQ for ampicillin was achieved 10 mg L−1 for Luna® Omega Polar C18 and 5 mg L−1 for Arion® Polar C18 using 20 µL of a solution containing 0.24 mg mL−1 of cephalexin as an internal standard. A number of articles dealing with the determination of ampicillin is limited, therefore, this study showed the HPLC method suitable for the determination of AMP in human blood plasma from patient who underwent elective cardiac surgical revascularization. In addition, the determination of AMP was also performed for the first time using an Arion® Polar C18 column, which effectively separated AMP from other compounds present in human blood plasma. This new polar-endcapped phase can help in separation of polar antibiotics or other polar compounds, which are unsuccessfully separated on conventional C18 column, and thus can help during method development.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Separation of ampicillin on polar-endcapped phase: Development of the HPLC method to achieve its correct dosage in cardiac surgery

Kubíčková

Rácová

Strojil

et al. 2023

AChrom

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“…Compared with SPE, DLLME is easier to implement with rapidity and more environmentally friendly, because it doesn’t need much solvent to elute targets and only needs common laboratory equipment [ 28 ]. DLLME also has a high potential for coupling with other sample pretreatment methods and analytical instruments [ 29 , 30 ].…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Determination of Sulfonamides Antibiotics in Environmental Water and Seafood Samples Using Ultrasonic-Assisted Dispersive Liquid-Liquid Microextraction Coupled with High Performance Liquid Chromatography

Wang

Ji³

et al. 2022

Molecules

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The residues and abuse of antibiotics have seriously endangered ecological balance and human health; meanwhile, antibiotics determination is very difficult because of their low levels and multiple categories in complicated matrices. Appropriate sample pretreatment is usually imperative to enrich (ultra)trace antibiotics and eliminate matrix interference prior to chromatographic analysis. Dispersive liquid-liquid microextraction (DLLME) has become an ideal pretreatment technique owing to its simplicity, effectiveness, low-consumption, etc. In this work, an ultrasonic-assisted DLLME (UA-DLLME) was developed for the simultaneous extraction of seven sulfonamides (SAs) antibiotics in environmental water and seafood samples coupled with HPLC-DAD determination. Several parameters affecting UA-DLLME efficiency were systematically optimized, and consequently the SAs were separated and detected within 14.5 min. The obtained limits of detection (LODs) and limits of quantification (LOQs) ranged from 0.7–7.8 μg/L and 2.4–26.0 μg/L for three water samples (seawater, aquaculture wastewater and lake water) and two seafood samples (pomfrets and shrimps). High recoveries (80.0–116.0%) with low relative standard deviations (0.1–8.1%) were achieved for all the tested samples at three spiked levels. Notably, sulfadimethoxine was found at 24.49 μg/L in one seawater sample. The facile, robust and benign DLLME-HPLC method demonstrated promising perspectives for multiresidue analysis of antibiotics.

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“…SPE includes off-line 13–15 and on-line methods. 16–18 The use of on-line techniques has made the development of faster methods possible by reducing the sample preparation time and thus increasing the sample throughput. It can decrease the risk of contamination of the sample or sample extract, eliminate the analyse losses by evaporation or by degradation during sample preconcentration, and improve precision and accuracy.…”

Section: Introductionmentioning

confidence: 99%

“…It can decrease the risk of contamination of the sample or sample extract, eliminate the analyse losses by evaporation or by degradation during sample preconcentration, and improve precision and accuracy. On-line methods are SPE-HPLC, 16 SPE-atomic fluorescence spectroscopy (AFS), 17 and SPE-UV 19 etc. On-line SPE was finished with manually control the injection valve.…”

Section: Introductionmentioning

confidence: 99%

On-line separation/analysis of Rhodamine B dye based on a solid-phase extraction high performance liquid chromatography self-designed device

Liu

Zhu

et al. 2021

RSC Adv.

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Lab-In-Syringe for automated double-stage sample preparation by coupling salting out liquid-liquid extraction with online solid-phase extraction and liquid chromatographic separation for sulfonamide antibiotics from urine

Cited by 43 publications

References 36 publications

Separation of ampicillin on polar-endcapped phase: Development of the HPLC method to achieve its correct dosage in cardiac surgery

Separation of ampicillin on polar-endcapped phase: Development of the HPLC method to achieve its correct dosage in cardiac surgery

Simultaneous Determination of Sulfonamides Antibiotics in Environmental Water and Seafood Samples Using Ultrasonic-Assisted Dispersive Liquid-Liquid Microextraction Coupled with High Performance Liquid Chromatography

On-line separation/analysis of Rhodamine B dye based on a solid-phase extraction high performance liquid chromatography self-designed device

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