Lab-on-a-chip platforms based on highly sensitive nanophotonic Si biosensors for single nucleotide DNA testing

Río, José Sánchez del; Carrascosa, Laura G.; Blanco, F.; Moreno, Miguel; Berganzo, J.; Calle, A.; Domı́nguez, Carlos; Lechuga, Laura M.

doi:10.1117/12.713977

Cited by 6 publications

(3 citation statements)

References 19 publications

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“…30 Once there is molecular interaction, the immobilization surface can be renewed by applying an appropriate chemical with the intention of eliminating target DNA samples except denaturing the probe DNA (p-DNA). 31 In Fig. 1, light acceptance cone is the angle of an incident light hitting the fiber core and totally reflected by the cladding.…”

Section: Design Methodologymentioning

confidence: 99%

“…These sequence orientation specification data in 916delTT and 6174delT are tabulated in Tables 1 and 2, respectively. 2,31,34 In Tables 1 and 2, p-DNA sequence (sh-DNA), target fully complementary sequence (mr-DNA), and target mismatch sequence (wt-DNA) are shown in bold, italic, and bold italic, respectively, whereas control target sequence (mf-DNA) is revealed in black.…”

Section: Designated Dna Sequences For Detection Of Breast Cancermentioning

confidence: 99%

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Numerical modeling of graphene-coated fiber optic surface plasmon resonance biosensor for BRCA1 and BRCA2 genetic breast cancer detection

et al. 2019

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A numerical illustration of a hybrid design and numerical analysis of graphene-coated fiber-optic surface plasmon resonance (SPR) biosensor for BRCA-1 and BRCA-2 genetic breast cancer detection is provided. Two specific mutations named 916delTT in BRCA-1 gene and 6174delT in BRCA-2 gene are being selected for detection of breast cancer numerically. This sensor is based on attenuated total reflection (ATR) method to detect individual point mutations in BRCA-1 and BRCA-2 genes. Based on the numerically obtained results, a momentous change is present in the SPR angle (minimum 35% more) and surface resonance frequency (SRF) (minimum 36% more) for probe DNA with various concentrations of target DNA corresponding to the mutation of the BRCA-1 and BRCA-2 genes. The variation of the SPR angle and SRF for mismatched DNA strands in BRCA-1 and BRCA-2 genes is quite negligible, whereas that for complementary DNA strands is considerable. This considerable change is essential for proper detection of genetic biomarkers (916delTT and 6174delT) for early breast cancer. To the best of our knowledge, this is the first demonstration of such an adept biosensor for detecting BRCA1 and BRCA2 genetic breast cancer. Here, we used graphene as bimolecular acknowledgement element for improving sensor performance. At the end of the article, the performance in terms of sensitivity is analyzed. Therefore, the proposed biosensor opens a window toward detection of early detection of BRCA-1 and BRCA-2 genetic breast cancers.

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Section: Design Methodologymentioning

confidence: 99%

Section: Designated Dna Sequences For Detection Of Breast Cancermentioning

confidence: 99%

Numerical modeling of graphene-coated fiber optic surface plasmon resonance biosensor for BRCA1 and BRCA2 genetic breast cancer detection

et al. 2019

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“…Other silanes carry SH- as the functional groups, such as MPTS (Mercaptopropyltriethoxysilane). Thiolated monolayers allow rapid and simple immobilization of SH-DNA probes by different chemistries without using any crosslinker or other reagent, such as disulphide bond linkage (Sánchez del Rio et al, 2007), or through thiol-ene click photochemistry (Escorihuela et al, 2014a). The latter one can also be also employed by activation of the thiol group at the probe to further reaction with different functional groups at the sensor surface, such as alkenylated and alkynylated surfaces by forming thiol-ene and thiol-yne links, respectively (Bañuls et al, 2019).…”

Section: Nucleic-acid Based Label-free Optical Biosensorsmentioning

confidence: 99%

Advanced Evanescent-Wave Optical Biosensors for the Detection of Nucleic Acids: An Analytic Perspective

et al. 2019

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Evanescent-wave optical biosensors have become an attractive alternative for the screening of nucleic acids in the clinical context. They possess highly sensitive transducers able to perform detection of a wide range of nucleic acid-based biomarkers without the need of any label or marker. These optical biosensor platforms are very versatile, allowing the incorporation of an almost limitless range of biorecognition probes precisely and robustly adhered to the sensor surface by covalent surface chemistry approaches. In addition, their application can be further enhanced by their combination with different processes, thanks to their integration with complex and automated microfluidic systems, facilitating the development of multiplexed and user-friendly platforms. The objective of this work is to provide a comprehensive synopsis of cutting-edge analytical strategies based on these label-free optical biosensors able to deal with the drawbacks related to DNA and RNA detection, from single point mutations assays and epigenetic alterations, to bacterial infections. Several plasmonic and silicon photonic-based biosensors are described together with their most recent applications in this area. We also identify and analyse the main challenges faced when attempting to harness this technology and how several innovative approaches introduced in the last years manage those issues, including the use of new biorecognition probes, surface functionalization approaches, signal amplification and enhancement strategies, as well as, sophisticated microfluidic solutions.

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Emerging optofluidic technologies for point-of-care genetic analysis systems: a review

Brennan¹,

Justice²,

Corbett³

et al. 2009

Anal Bioanal Chem

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This review describes recently emerging optical and microfluidic technologies suitable for point-of-care genetic analysis systems. Such systems must rapidly detect hundreds of mutations from biological samples with low DNA concentration. We review optical technologies delivering multiplex sensitivity and compatible with lab-on-chip integration for both tagged and non-tagged optical detection, identifying significant source and detector technology emerging from telecommunications technology. We highlight the potential for improved hybridization efficiency through careful microfluidic design and outline some novel enhancement approaches using target molecule confinement. Optimization of fluidic parameters such as flow rate, channel height and time facilitates enhanced hybridization efficiency and consequently detection performance as compared with conventional assay formats (e.g. microwell plates). We highlight lab-on-chip implementations with integrated microfluidic control for "sample-to-answer" systems where molecular biology protocols to realize detection of target DNA sequences from whole blood are required. We also review relevant technology approaches to optofluidic integration, and highlight the issue of biomolecule compatibility. Key areas in the development of an integrated optofluidic system for DNA hybridization are optical/fluidic integration and the impact on biomolecules immobilized within the system. A wide range of technology platforms have been advanced for detection, quantification and other forms of characterization of a range of biomolecules (e.g. RNA, DNA, protein and whole cell). Owing to the very different requirements for sample preparation, manipulation and detection of the different types of biomolecules, this review is focused primarily on DNA-DNA interactions in the context of point-of-care analysis systems.

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Lab-on-a-chip platforms based on highly sensitive nanophotonic Si biosensors for single nucleotide DNA testing

Cited by 6 publications

References 19 publications

Numerical modeling of graphene-coated fiber optic surface plasmon resonance biosensor for BRCA1 and BRCA2 genetic breast cancer detection

Numerical modeling of graphene-coated fiber optic surface plasmon resonance biosensor for BRCA1 and BRCA2 genetic breast cancer detection

Advanced Evanescent-Wave Optical Biosensors for the Detection of Nucleic Acids: An Analytic Perspective

Emerging optofluidic technologies for point-of-care genetic analysis systems: a review

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