Lab scale production and optimization of PHB Biopolymer using orange peel by Pseudomonas spp. and Bacillus spp.

Parwar, Shivani; Gomashe, Ashok V.; Rohi, Mayur N.

doi:10.31142/ijtsrd11100

Cited by 2 publications

(5 citation statements)

References 3 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Decreasing the rate of acetyl-CoA admission into tricarboxylic acid generates greater targeting to PHA biosynthesis pathways, consequently increasing PHA production. 83 In some works, the production of PHA was evaluated using simple orange peel extracts (Sukan et 68,84,85 Sukan, Roy and Keshavarz performed cultures of Bacillus subtilis OK2 in a medium containing microwave-pretreated orange peel solutions, acid or water suspension in the presence of glucose and citrate. 68 The highest content of P3HB was obtained with orange peel suspensions in water.…”

Section: Proposed Model For Brazil: Cross-sectorial Biorefinery Citru...mentioning

confidence: 99%

“…strains grown on orange peel suspensions in distilled water. 84 Four cultures (two per strain) were performed, all with added glucose. The amino acids lysine and histidine were added to one flask of each strain.…”

Section: Proposed Model For Brazil: Cross-sectorial Biorefinery Citru...mentioning

confidence: 99%

“…; Valdez‐Calderon et al .) 68,84,85 . Sukan, Roy and Keshavarz performed cultures of Bacillus subtilis OK2 in a medium containing microwave‐pretreated orange peel solutions, acid or water suspension in the presence of glucose and citrate 68 .…”

Section: Research On the Use Of Citrus Residues In The Production Of Phamentioning

confidence: 99%

“…and Pseudomonas sp. strains grown on orange peel suspensions in distilled water 84 . Four cultures (two per strain) were performed, all with added glucose.…”

Section: Research On the Use Of Citrus Residues In The Production Of Phamentioning

confidence: 99%

“…Several methods of pre‐treatment of fresh citrus pulp and fresh orange peels can be found in the literature aiming to use these substrates as a carbon sources for production of PHA: mechanical, 98 microwave, 68,70 chemical, 89 biological 92 or combined 69 methods. While simpler methods exist that utilize extracts from mechanical trituration and water suspension, 68,75,84 hydrolysis steps stand out for their ability to release pectin components, thereby enhancing bacterial metabolism. If the bacterial strains used in the bioprocess are able to use pectin as carbon source, a partial hydrolysis of citrus residues could be applied just to reduce the viscosity of the high concentrate feeding solution.…”

Section: Research On the Use Of Citrus Residues In The Production Of Phamentioning

confidence: 99%

See 4 more Smart Citations

Cross‐sectoral citrus sugarcane biorefinery: polyhydroxyalkanoates production in Brazil

Kanbe,

da Silva,

Gomez

2023

Biofuels Bioprod Bioref

View full text Add to dashboard Cite

Polyhydroxyalkanoates (PHA) are naturally occurring polyesters accumulated by bacteria under conditions of inorganic nutrient limitations and excess carbon. They offer renewable and biodegradable alternatives to petroleum‐based polymers, especially those used in transient single‐use applications. However, PHA still faces high production costs, primarily linked to the carbon source, lower productivity in processes and an early stage of technical development in comparison with well‐established fossil‐based polymers, which challenge its transition to a true commodity market. Recent research has highlighted the potential of renewable, inexpensive, abundant and non‐edible carbon‐rich residues from agriculture, municipal solid waste and industrial byproducts as alternative carbon sources in developing the production process. Recognizing Brazil's position as the leading producer of both citrus and sugarcane, this review introduces a novel citrus–sugarcane biorefinery model for PHA production, capitalizing on the geographical and operational synergies of both sectors. The proposed model integrates wet citrus pulp as a second‐generation carbon source, sugarcane industry fermenters, waste management, solvents and renewable energy from both sectors. Primary challenges involve the need for efficient, low‐cost biomass pre‐treatment to remove limonene and partially extract citric pectin, coupled with the partial hydrolysis of pectin to enhance solubility and reduce viscosity. Additional considerations include determining citrus biomass composition and sugar consumption, screening bacterial diversity for optimal PHA production, and deepening our understanding of the metabolic pathways underpinning the catabolism of citrus residues. Future research should focus on enhancing PHA concentrations and diversity and finally conducting life cycle assessment studies to evaluate the sustainability and feasibility of this proposed model.

show abstract

Section: Proposed Model For Brazil: Cross-sectorial Biorefinery Citru...mentioning

confidence: 99%

Section: Proposed Model For Brazil: Cross-sectorial Biorefinery Citru...mentioning

confidence: 99%

Section: Research On the Use Of Citrus Residues In The Production Of Phamentioning

confidence: 99%

“…and Pseudomonas sp. strains grown on orange peel suspensions in distilled water 84 . Four cultures (two per strain) were performed, all with added glucose.…”

Section: Research On the Use Of Citrus Residues In The Production Of Phamentioning

confidence: 99%

Section: Research On the Use Of Citrus Residues In The Production Of Phamentioning

confidence: 99%

See 3 more Smart Citations

Cross‐sectoral citrus sugarcane biorefinery: polyhydroxyalkanoates production in Brazil

Kanbe,

da Silva,

Gomez

2023

Biofuels Bioprod Bioref

View full text Add to dashboard Cite

show abstract

PHB production by Bacillus megaterium strain MNSH1-9K-1 using low-cost media

Rivas-Castillo,

Valdez-Calderón,

Angeles-Padilla

et al. 2024

Braz J Microbiol

View full text Add to dashboard Cite

Lab scale production and optimization of PHB Biopolymer using orange peel by Pseudomonas spp. and Bacillus spp.

Cited by 2 publications

References 3 publications

Cross‐sectoral citrus sugarcane biorefinery: polyhydroxyalkanoates production in Brazil

Cross‐sectoral citrus sugarcane biorefinery: polyhydroxyalkanoates production in Brazil

PHB production by Bacillus megaterium strain MNSH1-9K-1 using low-cost media

Contact Info

Product

Resources

About