Label‐Free and Continuous‐Flow Ferrohydrodynamic Separation of HeLa Cells and Blood Cells in Biocompatible Ferrofluids

Zhao, Wujun; Zhu, Taotao; Cheng, Rui; Liu, Yuanyuan; He, Jian; Qiu, Hong; Wang, Lianchun; Nagy, Tamás; Querec, Troy D.; Unger, Elizabeth R.; Mao, Leidong

doi:10.1002/adfm.201503838

Cited by 87 publications

(90 citation statements)

References 57 publications

(47 reference statements)

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“…Furthermore, they showed this particular commercial ferrofluid was not detrimental to the viability of both cell types after 2 h of exposure. Recently, Zhao and Zhu et al [65] demonstrated the separation of HeLa cells (cervical carcinoma) and blood cells in a custom-made biocompatible ferrofluid with a moderate throughput (∼10 6 cells h -1 ) and high separation efficiency (> 99%). Liang et al [66] separated binary mixture of particles (5 and 15 μm) in EMG 408 ferrofluids.…”

Section: Review Of Applicationsmentioning

confidence: 99%

“…Similar viability tests of live yeast cells were also conducted in later reports. [67, 73] Recently, a water-based ferrofluid was synthesized by Zhao and Zhu et al [65] with its maghemite nanoparticles stabilized by graft copolymer (polymethyl methacrylate-polyethylene glycol), and a pH of 6.8. Cell viability tests showed consistently 100% viability for mouse blood cells, and ∼90% viability for HeLa cells across different concentrations of this ferrofluid (0.30%, 0.79% and 1.03% of volume fraction), after 2 h of exposure.…”

Section: Review Of Applicationsmentioning

confidence: 99%

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Label‐Free Microfluidic Manipulation of Particles and Cells in Magnetic Liquids

Zhao

Cheng

Miller

et al. 2016

Adv Funct Materials

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132

125

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Manipulating particles and cells in magnetic liquids through so-called “negative magnetophoresis” is a new research field. It has resulted in label-free and low-cost manipulation techniques in microfluidic systems and many exciting applications. It is the goal of this review to introduce the fundamental principles of negative magnetophoresis and its recent applications in microfluidic manipulation of particles and cells. We will first discuss the theoretical background of three commonly used specificities of manipulation in magnetic liquids, which include the size, density and magnetic property of particles and cells. We will then review and compare the media used in negative magnetophoresis, which include paramagnetic salt solutions and ferrofluids. Afterwards, we will focus on reviewing existing microfluidic applications of negative magnetophoresis, including separation, focusing, trapping and concentration of particles and cells, determination of cell density, measurement of particles' magnetic susceptibility, and others. We will also examine the need for developing biocompatible magnetic liquids for live cell manipulation and analysis, and its recent progress. Finally, we will conclude this review with a brief outlook for this exciting research field.

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Section: Review Of Applicationsmentioning

confidence: 99%

Section: Review Of Applicationsmentioning

confidence: 99%

Label‐Free Microfluidic Manipulation of Particles and Cells in Magnetic Liquids

Zhao

Cheng

Miller

et al. 2016

Adv Funct Materials

Self Cite

132

125

View full text Add to dashboard Cite

show abstract

“…The ferromicrofluidic platform was reported to have 99% size-based separation efficiency of both microparticles and live cells [54]. More recently, a study by Zhao et al [55] has demonstrated a low-cost, label-free and rapid, throughput ≈ 10 6 cells h −1…”

Section: Magnetic Forcementioning

confidence: 99%

Sorting and manipulation of biological cells and the prospects for using optical forces

Atajanov

Zhbanov

Yang

2018

Micro and Nano Syst Lett

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Contemporary biomedical research requires development of novel techniques for sorting and manipulation of cells within the framework of a microfluidic chip. The desired functions of a microfluidic chip are achieved by combining and integrating passive methods that utilize the channel geometry and structure, as well as active methods that include magnetic, electrical, acoustic and optical forces. Application of magnetic, electric and acoustics-based methods for sorting and manipulation have been and are under continuous scrutiny. Optics-based methods, in contrast, have not been explored to the same extent as other methods, since they attracted insufficient attention. This is due to the complicated, expensive and bulky setup required for carrying out such studies. However, advances in optical beam shaping and computer hardware, and software have opened up new opportunities for application of light to development of advanced sorting and manipulation techniques. This review outlines contemporary techniques for cell sorting and manipulation, and provides an in-depth view into the existing and prospective uses of light for cell sorting and manipulation.

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“…Active techniques reply on external electric 4 , magnetic 5,6 , acoustic 7 or optical 8 force fields, whereas passive techniques depend entirely on the channel geometry and intrinsic hydrodynamic forces, such as pinched flow fractionation 9 , deterministic lateral displacement 10 , hydrophoresis 11 , inertial microfluidics 12 and viscoelastic focusing 13 .…”

Section: Introductionmentioning

confidence: 99%

“…Because ferrofluids usually have a much higher magnetic susceptibility than that of paramagnetic solutions, permanent magnets or electromagnets are sufficient to induce enough magnetophoretic force to manipulate nonmagnetic particles in microfluidics 22 . Many previous studies have reported focusing and separation of microparticles and cells using ferrofluid in microfluidics 5,16,18,[23][24][25] . In those studies, the non-magnetic particle suspension needs to be first confined by a co-flowing sheath flow; and in the downstream an external magnetic field acts on the nonmagnetic particles and deflects them into different paths 16,25 .…”

Section: Introductionmentioning

confidence: 99%

A novel viscoelastic-based ferrofluid for continuous sheathless microfluidic separation of nonmagnetic microparticles

et al. 2016

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Separation of microparticles has found broad applications in biomedicine, industry and clinical diagnosis. In a conventional aqueous ferrofluid, separation of microparticles usually employs a sheath flow or two offset magnets to confine particle streams for downstream particle sorting. This complicates the fluid control, device fabrication, and dilutes the particle sample. In this work, we propose and develop a novel viscoelastic ferrofluid by replacing the Newtonian base medium of the conventional ferrofluid with non-Newtonian poly(ethylene oxide) (PEO) aqueous solution. The properties of both viscoelastic 3D focusing and negative magnetophoresis of the viscoelastic ferrofluid were verified and investigated. By employing the both properties in a serial manner, continuous and sheathless separation of nonmagnetic particles based on particle size has been demonstrated. This novel viscoelastic ferrofluid is expected to bring more flexibility and versatility to the design and functionality in microfluidic devices. Disciplines Engineering | Science and Technology Studies Abstract:Separation of microparticles has found broad applications in biomedicine, industry and clinical diagnosis. In a conventional aqueous ferrofluid, separation of microparticles usually employs a sheath flow or two offset magnets to confine particle streams for downstream particle sorting. This complicates the fluid control, device fabrication, and dilutes particle sample. In this work, we propose and develop a novel viscoelastic-based ferrofluid by replacing the Newtonian base medium of the conventional ferrofluid with non-Newtonian poly(ethylene oxide) (PEO) aqueous solution. The properties of both viscoelastic 3D focusing and negative magnetophoresis of the viscoelastic-based ferrofluid were verified and investigated. By employing the both properties in a serial manner, continuous and sheathless separation of non-magnetic particles based on particle size has been demonstrated. This novel viscoelastic ferrofluid is expected to bring more flexibility and versatility to the design and functionality in microfluidic devices.

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Label‐Free and Continuous‐Flow Ferrohydrodynamic Separation of HeLa Cells and Blood Cells in Biocompatible Ferrofluids

Cited by 87 publications

References 57 publications

Label‐Free Microfluidic Manipulation of Particles and Cells in Magnetic Liquids

Label‐Free Microfluidic Manipulation of Particles and Cells in Magnetic Liquids

Sorting and manipulation of biological cells and the prospects for using optical forces

A novel viscoelastic-based ferrofluid for continuous sheathless microfluidic separation of nonmagnetic microparticles

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