Label-free and enzyme-free detection of transcription factors with graphene oxide fluorescence switch-based multifunctional G-quadruplex-hairpin probe

Sensitive and Label-Free Fluorescent Detection of Transcription Factors Based on DNA-Ag Nanoclusters Molecular Beacons and Exonuclease III-Assisted Signal Amplification

“…In Lane 10, only a band of AgMBs and a slow-migrated stripe can be recorded. According to previous reports, the slow-migrated stripe was the complex of NF-κB p50 the dsNF-κB probe, , which could be cut and eluted for further TFs-related research . Importantly, it is clear that no reporter-6 can be released and AgMBs remained unchanged in the sample of Lane 10, which indicated that the digestion of dsNF-κB probe was blocked in the presence of TFs.…”

“…Nonetheless, the use of costly fluorescent labels make them unsuitable for large-scale detection. Since graphene oxide (GO) can absorb single-stranded DNA (ssDNA) and quench most of fluorophores, it was fused with molecular beacons to realize half-labeled or label-free detection of TFs, but the unspecific absorption between GO and biomolecules in biological media challenged the sensitivity of such nanosensors. , Some dsDNA specific dyes were integrated with rationally designed DNA probes to implement label-free detection of TFs, but they suffered from either limited sensibility caused by the nature of dyes , or the need for complicated annealing and deactivation processes . Hence, there are still great needs for exploring new TFs detection methods with high sensitivity, low cost, and high specificity …”

Interactions of DNA coated upconversion nanoparticles with 2D materials

“…6 Various types of sensors based on the interactions between a fluorescent dye and a quencher have been recently reported for biomolecular detection. [7][8] Typical examples involve the use of 2D materials as quenchers and organic dyes or nanoparticles as fluorescence sources. [9][10][11][12] In particular, nucleic acids, among other biomolecules, have been reported to interact with various 2D materials and this property has been used to design relevant biosensors.…”

Nitrogen and Sulfur Codoped Reduced Graphene Oxide as a General Platform for Rapid and Sensitive Fluorescent Detection of Biological Species

“…Graphene oxide (GO), a newly developed two-dimensional carbon material, has attracted intensive attention because of its relatively good electronic, thermal, and mechanical properties. − It is employed as a catalyst in many electrochemical biosensors. , Meanwhile, it is also used as a good luminescence quencher to construct many optical sensing systems. , Yang et al have reported a DNA detection method by exploring water-soluble GO as a fluorescent quencher for the first time in 2009 . After that, there are a lot of reports about the GO application in fluorescence and chemluminescnece sensing. − For instance, Gao et al have reported a polyethylene glycol protected GO-based protein detection method by aptamers, and decreased the detection limit to 0.0048 nM . Kanaras’s group has demonstrated a DNA sensor by up-conversion nanoparticles and GO in which excellent energy and electronic transfer is proved between nanoparticles and GO .…”