Label-free aptamer sensor based on silicon microring resonators

Park, Mi Kyoung; Kee, Jack Sheng; Quah, Jessie Yiying; Netto, Vivian; Song, Junfeng; Qing, Feng‐Ling; Fosse, Eric Mouchel La; Lo, Guo‐Qiang

doi:10.1016/j.snb.2012.08.078

Cited by 100 publications

(93 citation statements)

References 41 publications

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“…The signal that resulted from thrombin after 15 minutes incubation time was 317 ± 8.7 pm (mean ± SD). This binding signal is comparable to the value reported by Park et al for a SOI MRR system [13]. The intra-array variations found for aptamer capturing and thrombin binding were similar (CV≈ 2.8%).…”

Section: Resultssupporting

confidence: 88%

“…Fabrication of integrated MRRs has been described in the case of all three main IO main platforms, especially SOI [10,13,14] but also InP [15] and Si 3 N 4 [12,16,17] while realization was also reported for polymer systems [18,19]. The SOI platform offers a large refractive index contrast and therewith a small allowable bend radius and thus footprint.…”

Section: Introductionmentioning

confidence: 99%

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Performance of Arrayed Microring Resonator Sensors with the TriPleX Platform

GAJ¹,

RG²

2016

J Biosens Bioelectron

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Integrated-optical (bio) chemical sensors are more and more commercialized, mainly because of their high intrinsic sensitivity in combination with the possibilities they offer for integration in optofluidic devices. The microring resonator (MRR), for example, is a very feasible structure to be used as microarray sensor element for multiplex biosensing. Fabrication of MRRs has been described in the case of the TriPleX platform employing well-defined stacks of stoichiometric Si 3 N 4 and SiO 2 material. This platform allows for a wide variety of applications, due to its intrinsic low loss and its transparency for the VIS/NIR wavelength range. In the present paper, we describe the recent achievements in the characterization of arrays of these MRRs regarding temperature sensitivity, refractive index sensitivity and protein immobilization performance. Furthermore, the use of a reference channel/reference MRR is demonstrated in order to show the advantage of compensation of unintended change in temperature or sample composition. The refractive index sensitivity was determined to be 104 nm/RIU and the limit of detection was about 2 × 10 -6 RIU. MRRs appeared to behave very comparable (expressed as the coefficient of (intra-array) variation (CV)) regarding the response to temperature (CV≈0.3%) as well as refractive index (CV<0.1%). Furthermore, it is shown that protein immobilization onto the different MRRs of the same arrays can be realized in a comparable way (CV<3%). A good comparability (i.e., a low variation) in these aspects allows for the use of internal referencing in order to compensate for unintended change in temperature or refractive index and also helps to attain a high assay precision in the ultimate biosensing application. Ongoing research is focused on the integration of more functionality on chip and further miniaturization in order to allow for fabrication of complex, though affordable, sensor devices.

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Section: Resultssupporting

confidence: 88%

Section: Introductionmentioning

confidence: 99%

Performance of Arrayed Microring Resonator Sensors with the TriPleX Platform

GAJ¹,

RG²

2016

J Biosens Bioelectron

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“…LoD for anti-BSA recognition is calculated as U w /m detection , which results in 50.8 ng/ml and 5.2 ng/ml for Sample 'A' and Sample 'B', respectively. For the sake of comparison, the latter value is comparable to previous BSA-antiBSA experiments carried out with SU-8 nanopillar cells (2.3 ng/ml) [24], microring resonator biosensor (5.0 ng/ml) [27] and photonic crystal biosensor (26 ng/ml) [28]. Here we note that with FTIR one can obtain much better signal-to-noise ratio and resolution compared with other tools such as a conventional spectrometer.…”

Section: Resultssupporting

confidence: 83%

Silicon nanopillar arrays with SiO_2 overlayer for biosensing application

Choudhury¹,

Casquel²,

Bañuls³

et al. 2014

Opt. Mater. Express

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Abstract:We present the fabrication of silicon dioxide (SiO 2 ) coated silicon nanopillar array structures and demonstrate their application as sensitive optical biosensors. Colloidal lithography, plasma dry etching and deposition processes are used to fabricate SiO 2 coated Si nanopillar arrays with two different diameters and periods. Proof of concept bio recognition experiments are carried out with the bovine serum albumin (BSA)/antiBSA model system using Fourier transform visible and IR spectrometry (FT-VIS-IR) in reflection mode. A limit of detection (LoD) value of 5.2 ng/ml is estimated taking in to account the wavenumber uncertainty in the measurements.

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“…However, because of the time-limiting reason, the treatment was not performed in this work. however, a similar work based on a silicon nanophotonic platform has been successfully studied previously (5), where the radius of operational sensing elements (i.e. the micro-ring) is equal to that of the circularhole defect in our sensing chip.…”

Section: Experimental Analysismentioning

confidence: 97%