Label-free characterization of living human induced pluripotent stem cells by subcellular topographic imaging technique using full-field quantitative phase microscopy coupled with interference reflection microscopy

Sugiyama, Norikazu; Asai, Yasuyuki; Yamauchi, Toyohiko; Kataoka, Takuji; Ikeda, Takahiro; Iwai, Hidenao; Sakurai, Takashi; Mizuguchi, Yoshinori

doi:10.1364/boe.3.002175

Cited by 15 publications

(12 citation statements)

References 23 publications

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“…Nevertheless, recent advantages in RICM have shown that absolute distance measurements are possible in floating vesicles with the application of more wavelengths and quantitative image analysis (Schilling et al, 2004). Consequently dual wavelength or DWreflection interference microscopy is used recently for adhesion, vesicle dynamics and further developments of the technique (for examples see (Contreras-Naranjo and Ugaz, 2013, Limozin and Sengupta, 2009, Monzel et al, 2009, Mundinger et al, 2012, Sugiyama et al, 2012). One of the advantages of the interference methods is that they allow for label free investigation of the cell membrane.…”

Section: Interferometric Methodsmentioning

confidence: 99%

Mapping Membrane Topology Label Free and Corrected for Changes in the Refractive Index of the Membrane on a Nanometer Scale

Walter

Schiefermeier

Hobi

et al. 2014

Proceedings of the International Conference on Bioimaging

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The plasma membrane is the outer limit of the animal cell. As such, it is both a border separating inside from outside and a signaling platform for interactions with the surroundings. Among these interactions are extracellular matrix contacts and adhesion sites. The membrane and its contact sites together with the underlying cytoskeleton undergo constant remodeling, which leads to changes of the cell shape. In addition to spatial information micro-topographical maps provide, information about the z-dimension and describe the position of the plasma membrane with respect to the distance to a given substrate. Here we address how to measure height differences in the plasma membrane and how to create topographical maps of the plasma membrane with nanometer resolution. We address the currently used methodologies along with their advantages and drawbacks. Moreover, we delineate a label-free method to obtain topographic maps of the plasma membrane that are corrected for differences in the refractive index of the membrane utilizing an interferometric approach with multiple wavelengths and a normalization procedure to account for changes in the refractive index in the membrane.

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Section: Interferometric Methodsmentioning

confidence: 99%

Mapping Membrane Topology Label Free and Corrected for Changes in the Refractive Index of the Membrane on a Nanometer Scale

Walter

Schiefermeier

Hobi

et al. 2014

Proceedings of the International Conference on Bioimaging

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“…Well-known label-free imaging modalities, such as quantitative phase microscopy (QPM), are used to reconstruct nanoscale phase information within cells, including living cells [ 20 ]. Interference reflection microscopy (IRM), also sometimes referred to as Interference Reflection Contrast, or Surface Contrast Microscopy, is often used in conjunction with QPM [ 21 ]. This non-invasive label-free technique is employed in the study of cellular adhesions, migration, cell mitosis, and cytotoxicity amongst other parameters in stem cell cultures such as human induced pluripotent stem cells (hIPSCs).…”

Section: Main Textmentioning

confidence: 99%

Functional imaging for regenerative medicine

et al. 2016

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In vivo imaging is a platform technology with the power to put function in its natural structural context. With the drive to translate stem cell therapies into pre-clinical and clinical trials, early selection of the right imaging techniques is paramount to success. There are many instances in regenerative medicine where the biological, biochemical, and biomechanical mechanisms behind the proposed function of stem cell therapies can be elucidated by appropriate imaging. Imaging techniques can be divided according to whether labels are used and as to whether the imaging can be done in vivo. In vivo human imaging places additional restrictions on the imaging tools that can be used. Microscopies and nanoscopies, especially those requiring fluorescent markers, have made an extraordinary impact on discovery at the molecular and cellular level, but due to their very limited ability to focus in the scattering tissues encountered for in vivo applications they are largely confined to superficial imaging applications in research laboratories. Nanoscopy, which has tremendous benefits in resolution, is limited to the near-field (e.g. near-field scanning optical microscope (NSNOM)) or to very high light intensity (e.g. stimulated emission depletion (STED)) or to slow stochastic events (photo-activated localization microscopy (PALM) and stochastic optical reconstruction microscopy (STORM)). In all cases, nanoscopy is limited to very superficial applications. Imaging depth may be increased using multiphoton or coherence gating tricks. Scattering dominates the limitation on imaging depth in most tissues and this can be mitigated by the application of optical clearing techniques that can impose mild (e.g. topical application of glycerol) or severe (e.g. CLARITY) changes to the tissue to be imaged. Progression of therapies through to clinical trials requires some thought as to the imaging and sensing modalities that should be used. Smoother progression is facilitated by the use of comparable imaging modalities throughout the discovery and trial phases, giving label-free techniques an advantage wherever they can be used, although this is seldom considered in the early stages. In this paper, we will explore the techniques that have found success in aiding discovery in stem cell therapies and try to predict the likely technologies best suited to translation and future directions.

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“…It is nowadays quite straightforward to design a compact, highly sensitive phase microscope that can follow in real time the dynamics of cells. In the last decade, different teams [5][6][7][8][9][10][11][12][13] have played a major role in disseminating the concepts of quantitative phase microscopy (QPM) among the optical and biophysical community. They have applied this technique to the real-time characterization of cellular dynamics and their alteration in cases of diseases.…”

Section: Introductionmentioning

confidence: 99%

Diffraction phase microscopy: retrieving phase contours on living cells with a wavelet-based space-scale analysis

et al. 2014

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Abstract. We propose a two-dimensional (2-D) space-scale analysis of fringe patterns collected from a diffraction phase microscope based on the 2-D Morlet wavelet transform. We show that the adaptation of a ridge detection method with anisotropic 2-D Morlet mother wavelets is more efficient for analyzing cellular and high refractive index contrast objects than Fourier filtering methods since it can separate phase from intensity modulations. We compare the performance of this ridge detection method on theoretical and experimental images of polymer microbeads and experimental images collected from living myoblasts. © The Authors.Published by SPIE under a Creative Commons Attribution 3.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

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Label-free characterization of living human induced pluripotent stem cells by subcellular topographic imaging technique using full-field quantitative phase microscopy coupled with interference reflection microscopy

Cited by 15 publications

References 23 publications

Mapping Membrane Topology Label Free and Corrected for Changes in the Refractive Index of the Membrane on a Nanometer Scale

Mapping Membrane Topology Label Free and Corrected for Changes in the Refractive Index of the Membrane on a Nanometer Scale

Functional imaging for regenerative medicine

Diffraction phase microscopy: retrieving phase contours on living cells with a wavelet-based space-scale analysis

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