Label-free density-based detection of adipocytes of bone marrow origin using magnetic levitation

Sarigil, Oyku; Anıl-İnevi, Müge; Yilmaz, Esra; Meşe, Gülistan; Özçivici, Engin

doi:10.1039/c8an02503g

Cited by 38 publications

(30 citation statements)

References 94 publications

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“…Demonstrations of density measurements, [69,123] Quantification of cell number, [67] Assessmentofcellular aging, [70,73] Analysis of cells from anemic donors, [73] Diagnosis of sickle cell disease. [70,76] Human leukocytes Prohance 1.1 Assessmentofcellular activation of polymorphonuclear leukocytes (e.g.,caused by phagocytotic events of bacteria); [70] Diagnosis of sepsis by analyzing physical characteristics of levitated leukocytes (size, morphology,ormagnetic susceptibility) [75] Mouse bone marrow stem cells and osteoblasts Gadavist 1.09 Demonstrations of single-cell density measurements and separations [197] Mouse fibroblast (NIH-3T3) Gd•DTPA -M agnetict rap, [146] Self-assembly of 3D living architecture. [64,79] Sheep chondrocytes Omniscan-Self-assembly to fabricate tissue spheroids [66] Stem cell lines Gadavist Magnevist Omniscan Dotarem Multihance…”

Section: Fundamental Limits In the Size Of The Levitated Objectmentioning

confidence: 99%

Magnetic Levitation in Chemistry, Materials Science, and Biochemistry

et al. 2020

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All matter has density. The recorded uses of density to characterize matter date back to as early as ca. 250 BC, when Archimedes was believed to have solved “The Puzzle of The King's Crown” using density.[1] Today, measurements of density are used to separate and characterize a range of materials (including cells and organisms), and their chemical and/or physical changes in time and space. This Review describes a density‐based technique—magnetic levitation (which we call “MagLev” for simplicity)—developed and used to solve problems in the fields of chemistry, materials science, and biochemistry. MagLev has two principal characteristics—simplicity, and applicability to a wide range of materials—that make it useful for a number of applications (for example, characterization of materials, quality control of manufactured plastic parts, self‐assembly of objects in 3D, separation of different types of biological cells, and bioanalyses). Its simplicity and breadth of applications also enable its use in low‐resource settings (for example—in economically developing regions—in evaluating water/food quality, and in diagnosing disease).

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Section: Fundamental Limits In the Size Of The Levitated Objectmentioning

confidence: 99%

Magnetic Levitation in Chemistry, Materials Science, and Biochemistry

et al. 2020

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“…In particular, 3D cell cultures hold great potential to mimic tumors' cellular Magnetic levitation (MagLev) was also adopted for mass density measurements. Durmus et al [29] successfully employed this technique in 2015, and the most recent application was presented by Sarigil et al [30]. However, it can only be applied for the analysis of small samples, such as single cells.…”

Section: Introductionmentioning

confidence: 99%

A Reliable Flow-Based Method for the Accurate Measure of Mass Density, Size and Weight of Live 3D Tumor Spheroids

Cristaldi¹,

Sargenti²,

Bonetti³

et al. 2020

Micromachines

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Gathering precise information on mass density, size and weight of cells or cell aggregates, is crucial for applications in many biomedical fields with a specific focus on cancer research. Although few technical solutions have been presented for single-cell analysis, literature does not cover this aspect for 3D models such as spheroids. Since the research interest on such samples is notably rising, here we describe a flow-apparatus, and the associated physical method and operative protocol for the accurate measurements of mass density, size and weight. The technique is based on the detection of the terminal velocity of a free-falling sample into a specifically conceived analysis flow-channel. Moreover, in order to demonstrate the accuracy and precision of the presented flow-device, analyses were initially carried out on standardized polystyrene beads. Finally, to display the application of the proposed system for biological samples, mass density, size and weight of live SW620 tumor spheroids were analyzed. The combined measurements of such parameters can represent a step toward a deeper understanding of 3D culture models.

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“…known have distinct single-cell density [10], were co-cultured with magnetic levitation. In this experiment, adipogenic cells, which were expected to be positioned higher level due to their lipid accumulation and hence low cell density, were taken into magnetic levitation culture and after 24 hours, D1 ORL UVA stem cells were added to the culture (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…In principle, cells, most of which are diamagnetic objects, are pushed away from the high magnetic field region to the low magnetic field region in the paramagnetic medium, whereby a magnetic force equal to gravitational force is generated on the cell in a label-free manner and the actual weightlessness condition is simulated. Magnetic levitation technique was firstly applied to living cells for cell separation and single-cell density measurement [10][11][12][13]. The technique has a serious potential to provide an important opportunity to study the effects of the weightlessness environment with its use for cell culture.…”

Section: Introductionmentioning

confidence: 99%

Biofabrication of Cellular Structures Using Weightlessness as a Biotechnological Tool

Anıl-İnevi

Yalcin-Ozuysal

Sarigil

et al. 2019

2019 9th International Conference on Recent Advances in Space Technologies (RAST)

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Gravity is an important biomechanical signal effecting the morphology and function of organisms. Reduction of gravitational forces, as experienced during spaceflight, cause alterations in the biological systems. Magnetic levitation technique is one of the most recent ground-based technology to mimic weightlessness environment. In addition to providing a platform to investigate biological effects of the weightlessness, this platform presents a novel opportunity to biofabricate 3dimensional (3D) structures in a scaffold-and nozzle-free fashion. In this study, various controllable self-assembled 3D living structures were fabricated via magnetic levitation technique. This strategy may offer an easy and cost-effective opportunity for a wide range of space biotechnology researches.

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Label-free density-based detection of adipocytes of bone marrow origin using magnetic levitation

Abstract: The first report on application of magnetic levitation technology for detection of adipogenic cells based on single cell density measurement.

Cited by 38 publications

References 94 publications

Magnetic Levitation in Chemistry, Materials Science, and Biochemistry

Magnetic Levitation in Chemistry, Materials Science, and Biochemistry

A Reliable Flow-Based Method for the Accurate Measure of Mass Density, Size and Weight of Live 3D Tumor Spheroids

Biofabrication of Cellular Structures Using Weightlessness as a Biotechnological Tool

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