Label-Free Detection of Bovine Serum Albumin Protein Based on SiO<SUB>2</SUB>/Au Nanoshells as Near-Infrared Surface-Enhanced Raman Spectroscopy Nanoprobe

Analysis of Biomolecules Based on the Surface Enhanced Raman Spectroscopy

“…Bovine serum albumin (BSA) was chosen as a model molecule to research the enhancement of SERS-active substrates in label-free detection. Noble metallic nanomaterials such as gold nanocylinders [ 22 ] and SiO 2 /Au nanoshells [ 17 ] were used as substrates for BSA detection. To keep proteins in their natural structure and conformation, in-liquid SERS detection of protein was based on nanoparticle aggregating as SERS-active hot spots for label-free detection [ 27 ].…”

“…With the development of nanotechnology, increasing varieties of nanomaterials were discovered, including noble metal (e.g., Au and Ag) and transition metal (e.g., Co and Pt) with different material morphologies, such as nanoparticles (NPs), nanoflowers, nanoclusters, and nanostars. Due to the synergistic effects, many nanohybrids were designed with non-metallic material, such as SiO 2 [ 17 ] and graphene oxide (GO) [ 18 ]. Numerous complex structures were designed to enhance the SERS signal, containing hexagonal-packed lotus seedpod like array substrate [ 19 ], Fe 3 O 4 -Au core-shell NPs with branched gold shell [ 20 ] and Si nanopillars (SiNPLs)@silver nanoparticles (AgNPs) [ 21 ], etc.…”