Analysis of Biomolecules Based on the Surface Enhanced Raman Spectroscopy

Abstract

Analyzing biomolecules is essential for disease diagnostics, food safety inspection, environmental monitoring and pharmaceutical development. Surface-enhanced Raman spectroscopy (SERS) is a powerful tool for detecting biomolecules due to its high sensitivity, rapidness and specificity in identifying molecular structures. This review focuses on the SERS analysis of biomolecules originated from humans, animals, plants and microorganisms, combined with nanomaterials as SERS substrates and nanotags. Recent advances in SERS detection of target molecules were summarized with different detection strategies including label-free and label-mediated types. This comprehensive and critical summary of SERS analysis of biomolecules might help researchers from different scientific backgrounds spark new ideas and proposals.

Theoretical and Experimental Studies of Ti3C2 MXene for Surface-Enhanced Raman Spectroscopy-Based Sensing

“…Due to its advantages of fast detection speed, nondestructive, high sensitivity, high accuracy, and trace detection, SERS technology is widely applied in food safety, 1 − 3 biosensor, 4 , 5 environmental analysis, 6 and biomolecule analysis. 7 Substrate materials achieve a tremendous SERS enhancement mainly through two well-known mechanisms, i.e., electromagnetic mechanism (EM) and chemical mechanism (CM). 8 , 9 Among them, EM is determined to the significant enhancement of the local electromagnetic field due to the localized surface plasmon resonance effect (LSPR) while CM depends on the charge transfer (CT) between the adsorbed molecules and substrate materials.…”

Surface-Enhanced Raman Spectroscopy for Monitoring Extravirgin Olive Oil Bioactive Components

“…is technique is based on the use of metallic nano-objects (such as Au or Ag nanoparticles, nanorods, and so on) or nanopatterned substrates characterized by a well-suited surface plasmon resonance (i.e., oscillations of surface electrons of metal nanostructures induced by the impinging electromagnetic radiation), thanks to the interaction among molecules, nanomaterials, and light. In fact, the electromagnetic (EM) effect, related to the presence of the plasmon resonance, and the chemical effect, resulting from the interaction between molecules with Raman active modes and the nanostructures, concurrently work in SERS for giving rise to the enhancement of the otherwise weak Raman signals [25,26]. Actually, the interaction between molecules and nanomaterials changes the cross section of the Raman effect and sometimes also of the fluorescence emission, thus resulting also in a reduction of the fluorescence signal [24].…”

Prospects for Raman spectroscopy in cardiology

“…Для реализации ГКР-эффекта используют широкий инструментарий в виде частиц и структур металлов (золота, серебра, меди), обладающих способностью генерировать колебания свободных электронов, усиливающих многократно (до 10 10 раз) КР-сигнал [9]. Подобные частицы называют ГКРзондами или ГКР-наносенсорами.…”