Label-free detection of cytochrome <i>C</i> by a conducting polymer-based impedimetric screen-printed aptasensor

Shafaat, Atefeh; Faridbod, Farnoush; Ganjali, Mohammad Reza

doi:10.1039/c7nj03844e

Cited by 24 publications

(14 citation statements)

References 42 publications

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“…The impedimetric techniques have been used in monitoring the reactions catalyzed by enzymes or the biorecognition events of whole cells and microorganisms, antibodies, specific binding proteins, receptors, nucleic acids, lectins, etc. [ 78 , 79 ]. Porfireva et al developed an impedimetric DNA sensor based on electropolymerization of proflavine layer for the detection of anthracyclines.…”

Section: Classification Of Biosensorsmentioning

confidence: 99%

Emerging biosensors in detection of natural products

Piroozmand

Mohammadipanah

Faridbod

2020

Synthetic and Systems Biotechnology

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Natural products (NPs) are a valuable source in the food, pharmaceutical, agricultural, environmental, and many other industrial sectors. Their beneficial properties along with their potential toxicities make the detection, determination or quantification of NPs essential for their application. The advanced instrumental methods require time-consuming sample preparation and analysis. In contrast, biosensors allow rapid detection of NPs, especially in complex media, and are the preferred choice of detection when speed and high throughput are intended. Here, we review diverse biosensors reported for the detection of NPs. The emerging approaches for improving the efficiency of biosensors, such as microfluidics, nanotechnology, and magnetic beads, are also discussed. The simultaneous use of two detection techniques is suggested as a robust strategy for precise detection of a specific NP with structural complexity in complicated matrices. The parallel detection of a variety of NPs structures or biological activities in a mixture of extract in a single detection phase is among the anticipated future advancements in this field which can be achieved using multisystem biosensors applying multiple flow cells, sensing elements, and detection mechanisms on miniaturized folded chips.

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Section: Classification Of Biosensorsmentioning

confidence: 99%

Emerging biosensors in detection of natural products

Piroozmand

Mohammadipanah

Faridbod

2020

Synthetic and Systems Biotechnology

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“…Recently, Ganjali et al constructed a novel and selective biosensor based on electropolymerized polypyrrole for the entrapment of cytochrome C aptamer on a screen printed electrode. It also exhibited a linear response over a concentration range of 10 p m to 1 n m and limit of detection 5 p m …”

Section: Biosensorsmentioning

confidence: 96%

Progress in the Development of Intrinsically Conducting Polymer Composites as Biosensors

Prajapati

Kandasubramanian

2019

Macro Chemistry & Physics

104

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Biosensors are analytical devices which find extensive applications in fields such as the food industry, defense sector, environmental monitoring, and in clinical diagnosis. Similarly, intrinsically conducting polymers (ICPs) and their composites have lured immense interest in bio‐sensing due to their various attributes like compatibility with biological molecules, efficient electron transfer upon biochemical reactions, loading of bio‐reagent, and immobilization of biomolecules. Further, they are proficient in sensing diverse biological species and compounds like glucose (detection limit ≈0.18 nm), DNA (≈10 pm), cholesterol (≈1 µm), aptamer (≈0.8 pm), and also cancer cells (≈5 pm mL−1) making them a potential candidate for biological sensing functions. ICPs and their composites have been extensively exploited by researchers in the field of biosensors owing to these peculiarities; however, no consolidated literature on the usage of conducting polymer composites for biosensing functions is available. This review extensively elucidates on ICP composites and doped conjugated polymers for biosensing functions of copious biological species. In addition, a brief overview is provided on various forms of biosensors, their sensing mechanisms, and various methods of immobilizing biological species along with the life cycle assessment of biosensors for various biosensing applications, and their cost analysis.

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“…A range of electrode modifications are used for Faradaic sensors including nanomaterials 204 , SAMs 25, 57, 205-206 and polymers. 178,[207][208][209] This layer coating the electrode must either be conductive or have sufficient gaps to allow for electron transfer. For this reason, when opting for alkanethiols, a mixed SAM layer is often chosen.…”

Section: Faradaic Vs Non-faradaic Sensorsmentioning

confidence: 99%

Electrochemical Impedance Spectroscopy (EIS) Based Label-Free Immunosensors

Robinson

Juska

O’Riordan

2022

Preprint

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Electrochemical Impedance Spectroscopy (EIS) is a surface sensitive technique which examines the impedance of an electrochemical cell over a range of frequencies. By immobilising an antigen or antibody to the electrode surface, EIS shows potential for highly specific and sensitive immunosensor performance. Following an introduction into antibodies and EIS, this review presents immobilisation techniques for an immunosensor’s bioreceptor. It highlights electrode modifications such as self-assembled monolayers (SAMs), polymers and nanostructures for anchoring the bioreceptor to the surface. Stable immobilisation directly to these modifications is not always possible. Immobilisation techniques to these modified surfaces are explored including cross-linking and controlled orientation. EIS allows for label-free detection by monitoring the change in charge-transfer resistance or capacitance caused by changes in the electrode surface. Label-free immunosensors are the focus of this paper as they show potential for point-of-care (POC) devices.

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Label-free detection of cytochrome C by a conducting polymer-based impedimetric screen-printed aptasensor

Abstract: In this study we have introduced a new sensitive and selective biosensor for the determination of cytochrome C (Cyt C) as a biomarker for cell apoptosis.

Cited by 24 publications

References 42 publications

Emerging biosensors in detection of natural products

Emerging biosensors in detection of natural products

Progress in the Development of Intrinsically Conducting Polymer Composites as Biosensors

Electrochemical Impedance Spectroscopy (EIS) Based Label-Free Immunosensors

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