Label-free detection of hybridization of oligonucleotides by oblique-incidence reflectivity difference method

Yuan, Kun; Xu, Wenju; Lu, Haojie; Wen, Jiajia; Lü, Huibin; Zhou, Yongqiang; Jin, Kuijuan; Yang, Ge; Li, Wei; Ruan, Kangcheng

doi:10.1007/s11433-010-4024-z

Cited by 17 publications

(4 citation statements)

References 13 publications

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“…It is able to monitor surface/interface processes in a real-time and label-free form by measuring the phase and amplitude variations of the s and p components of the elliptically polarized reflected light caused by a change in the surface/interface dielectric constant . This technology has been employed to monitor oxide film growth, molecular adsorption at gas/solid interfaces, and the potential distribution at transparent electrode/solution interfaces, enabling label-free detection by microarray chips since its establishment. − …”

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confidence: 99%

Reusable OIRD Microarray Chips Based on a Bienzyme-Immobilized Polyaniline Nanowire Forest for Multiplexed Detection of Biological Small Molecules

et al. 2021

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Quantitative detection of multiple biological small molecules is critical for health evaluation and disease diagnosis. In this study, a microarray chip featuring a bienzyme-immobilized polyaniline nanowire forest on fluorine-doped tin oxide (bienzyme-PANI/FTO) is developed for this purpose. On such a chip, the target molecules are oxidized under the catalysis of their attached oxidases to produce hydrogen peroxide, which further induces the partial oxidation of local PANI nanowires in the presence of horseradish peroxidase (HRP) enzyme. The redox state change of PANI nanowires is monitored by the oblique incident reflectivity difference (OIRD) technique in a real-time and wireless manner, thus allowing for quantitative analysis of the target molecules. As typical model targets, hydrogen peroxide, glucose, lactic acid, and cholesterol are successfully detected with low detection limits, excellent specificities, and broad detection ranges, all of which fully meet the requirements for clinical analysis of human serum samples. Simultaneous detection of multiple targets on an individual chip is further demonstrated using the OIRD scanning mode. Meanwhile, by simple electrochemical reduction of the PANI nanowires, the chip is reusable for more than eight detection cycles without evident decay in its performance. The detection principle of this chip is also universal to other small molecules, and thus, it shows great promise as a valuable device to analyze biological small molecules.

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confidence: 99%

Reusable OIRD Microarray Chips Based on a Bienzyme-Immobilized Polyaniline Nanowire Forest for Multiplexed Detection of Biological Small Molecules

et al. 2021

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“…9,10 In our previous works, we have label-free detected the protein and oligonucleotide microarrays by OIRD method. [11][12][13][14][15][16][17] In this paper, we report the label-free and real-time detections of the interactions and the reaction dynamic processes of swine IgG with goat anti-swine IgG.…”

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confidence: 99%

Label-free and real-time detections of the interactions of swine IgG with goat anti-swine IgG by oblique-incidence reflectivity difference technique

Wang

Dai

et al. 2012

Journal of Applied Physics

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With the oblique-incidence reflectivity difference (OIRD) technique, we successfully label-free detected the interaction of swine IgG with different concentrations of 0.125, 0.25, 0.5, 1 mg/ml, and 5 lg/ml goat anti-swine IgG, and real-time detected the reaction dynamic processes of 0.5 mg/ml swine IgG and goat anti-swine IgG with different concentration of 5, 10, and 20 lg/ml, respectively. The interaction times are about 2043, 1828, and 1347 s for the reactions of 0.5 mg/ml swine IgG and goat anti-swine IgG of 5, 10, and 20 lg/ml, respectively. By fitting the reaction dynamic curves, we obtained that the association constant of swine IgG and goat anti-swine IgG is 1620.77 M À1 ÁS À1 at temperature about 22 C. The experimental results demonstrate that the OIRD is a promising and competing method for label-free and real time detecting the biomolecular interactions and achieving the quantitative information of reaction kinetics. V

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“…[7−10] As mentioned in our previous studies, [9,10] the OIRD method measures the fractional difference between the reflectivity of the p-and s-polarized lights from a surface under the condition of oblique incidence, i.e., Δ p − Δ s . We have reported the use of the OIRD method to determine the kinetics of antigen-antibody interactions, [10−13] oligonucleotides hybridization, [14] dynamic processes of protein degradation, [15] protein-DNA interactions, [16] DNA hybridization, [17] and protein-protein interactions. [18,19] This method has been proven to be highly reproducible.…”

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confidence: 99%

Label-Free and Real-Time Monitor of Binding and Dissociation Processes between Protein A and Swine IgG by Oblique-Incidence Reflectivity Difference Method

He¹,

Liu²,

Dai³

et al. 2015

Chinese Phys. Lett.

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Life science has a need for detection methods that are label-free and real-time. In this paper, we have selected staphylococcal protein A (SPA) and swine immunoglobulin G (IgG), and monitor the bindings between SPA and swine IgG with different concentrations, as well as the dissociations of SPA-swine IgG complex in different pH values of phosphate buffer by oblique-incidence reflectivity difference (OIRD) in a label-free and real-time fashion. We obtain the ON and OFF reaction dynamic curves corresponding to the bindings and dissociations of SPA and swine IgG. Through our analysis of the experimental results, we have been able to obtain the damping coefficients and the dissociation time of SPA and swine IgG for different pH values of the phosphate buffer. The results prove that the OIRD technique is a competing method for monitoring the dynamic processes of biomolecule interaction and achieving the quantitative information of reaction kinetics.

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Label-free detection of hybridization of oligonucleotides by oblique-incidence reflectivity difference method

Cited by 17 publications

References 13 publications

Reusable OIRD Microarray Chips Based on a Bienzyme-Immobilized Polyaniline Nanowire Forest for Multiplexed Detection of Biological Small Molecules

Reusable OIRD Microarray Chips Based on a Bienzyme-Immobilized Polyaniline Nanowire Forest for Multiplexed Detection of Biological Small Molecules

Label-free and real-time detections of the interactions of swine IgG with goat anti-swine IgG by oblique-incidence reflectivity difference technique

Label-Free and Real-Time Monitor of Binding and Dissociation Processes between Protein A and Swine IgG by Oblique-Incidence Reflectivity Difference Method

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