Label-free detection of nucleic acid and protein microarrays by scanning Kelvin nanoprobe

Thompson, Michael; Cheran, Larisa-Emilia; Zhang, Mingquan; Chacko, Melissa; Huo, Hongliang; Sadeghi, Saman

doi:10.1016/j.bios.2004.06.022

Cited by 58 publications

(55 citation statements)

References 37 publications

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“…From the measurement data and modeling, the work function shift is about 50 meV/decade of DNA concentration. The work function shifts show the logarithmic dependence of DNA concentration, and are in reasonable range when compared to the measurement data in the literature [18][19][20]. However, there are differences in Figs.…”

Section: Resultssupporting

confidence: 75%

Analysis of Sensing Mechanisms in a Gold-Decorated SWNT Network DNA Biosensor

Ahn¹,

Kim²,

Lim³

et al. 2014

JSTS:Journal of Semiconductor Technology and Science

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Abstract-We show that carbon nanotube sensors with gold particles on the single-walled carbon nanotube (SWNT) network operate as Schottky barrier transistors, in which transistor action occurs primarily by varying the resistance of Au-SWNT junction rather than the channel conductance modulation. Transistor characteristics are calculated for the statistically simplified geometries, and the sensing mechanisms are analyzed by comparing the simulation results of the MOSFET model and Schottky junction model with the experimental data. We demonstrated that the semiconductor MOSFET effect cannot explain the experimental phenomena such as the very low limit of detection (LOD) and the logarithmic dependence of sensitivity to the DNA concentration. By building an asymmetric concentricelectrode model which consists of serially-connected segments of CNTFETs and Schottky diodes, we found that for a proper explanation of the experimental data, the work function shifts should be ~ 0.1 eV for 100 pM DNA concentration and ~ 0.4 eV for 100 µM.

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Section: Resultssupporting

confidence: 75%

Analysis of Sensing Mechanisms in a Gold-Decorated SWNT Network DNA Biosensor

Ahn¹,

Kim²,

Lim³

et al. 2014

JSTS:Journal of Semiconductor Technology and Science

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“…cyclic and square wave voltammetry, 163 scanning electrochemical microscopy (SECM), 164 detection of conduction changes of carbon nanotubes upon protein adsorption, 96 and the scanning Kelvin nanoprobe. 165,166 These electronic-based detection approaches have a high potential in the future microarray market, especially in the area of diagnostics, in which systems aiming at a direct read-out of electrical signals without the need of additional transducers are potential candidates for highly miniaturized or even handheld devices.…”

Section: Non-optical Detection Methodsmentioning

confidence: 99%

Optical microarray biosensing techniques

Bally

Halter

Vörös

et al. 2006

Surface & Interface Analysis

160

126

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Microarrays provide a powerful analytical tool for the simultaneous analyses of thousands of parameters, be they DNA or proteins, in a single experiment. However, a number of challenges continue to hinder the widespread application of microarray assays for analytical and diagnostic purposes. Advances in detection methods can help overcome some of these challenges by improving sensitivity and reliability in signal detection and by enabling real-time detection of binding events. Thus, the aim of this review is to highlight the most promising optical microarray biosensing techniques. The principles of techniques making use of labels, including scanner type, total internal reflection type, fiber-optics-based, and SPRenhanced fluorescence, are described, along with a brief summary of labeling strategies. State of the art label-free techniques, including imaging SPR and imaging ellipsometry, are also reviewed. Examples of microarray-based assays using each technique are given to illustrate both their usefulness and their limits of detection. Furthermore, the most competitive commercial microarray systems are presented and compared with one another in the context of their detection system. Finally, a discussion of the remaining challenges as well as trends and future applications of microarrays are presented in the context of optical sensing.

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“…However, the labeled technique has limitations due to the complications in sample preparation as well as the necessary usage of expensive optical systems and specialized, along with health risk. Currently, hybridization between a target DNA sequence and a complementary DNA sequence is monitored by using the label-free method, including quartz crystal microcantilever [8], genetic field-effect transistor (FET) [9,10], scanning probe microscopy [11], and surface plasmon resonance [12]. Compared with labeled techniques, the label-free electronic method is an excellent candidate for the application.…”

Section: Introductionmentioning

confidence: 99%