The hybridization and optimization of complementary DNA molecules on organic field-effect transistors

Gui, Haiyang; Wei, Bin; Wang, Jun

doi:10.1016/j.mssp.2014.10.020

Cited by 12 publications

(11 citation statements)

References 20 publications

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“…Currently only Gui et al have investigated elevated temperature operation of OTFT DNA sensors. 28 They investigated the effect of hybridization times at T ¼ 20 C, 45 C and 60 C on sensor sensitivity. For their particular DNA sequence, they found that optimal sensor response occurred at 45 C (13 C below their sequence T M ).…”

Section: Introductionmentioning

confidence: 99%

P and N type copper phthalocyanines as effective semiconductors in organic thin-film transistor based DNA biosensors at elevated temperatures

et al. 2019

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Section: Introductionmentioning

confidence: 99%

P and N type copper phthalocyanines as effective semiconductors in organic thin-film transistor based DNA biosensors at elevated temperatures

et al. 2019

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“…Since the DNA molecule had a negatively charged phosphoric acid group, which introduced a positive bias between the gate and the source, the target DNA could be immobilized by electrostatic induction. Gui et al [56] used the known single-stranded DNA (ssDNA) molecules as target DNA sequences and fixed them onto pentacene thin films by physical adsorption to form a dsDNA (double-stranded DNA, dsDNA) sensing unit that can quickly detect unknown DNA sequences. The authors also explored the effects of hybridization temperature and time on hybridization efficiency and device sensitivity.…”

Section: Immobilization Strategies Of Dnamentioning

confidence: 99%

Recent Advances in Immobilization Strategies for Biomolecules in Sensors Using Organic Field-Effect Transistors

Wang

Xiao

et al. 2020

Trans. Tianjin Univ.

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Organic field-effect transistors (OFETs) are fabricated using organic semiconductors (OSCs) as the active layer in the form of thin films. Due to its advantages of high sensitivity, low cost, compact integration, flexibility, and printability, OFETs have been used extensively in the sensing area. For analysis platforms, the construction of sensing layers is a key element for their efficient detection capability. The strategy used to immobilize biomolecules in these devices is especially important for ensuring that the sensing functions of the OFET are effective. Generally, analysis platforms are developed by modifying the gate/electrolyte or OSC/electrolyte interface using biomolecules, such as enzymes, antibodies, or deoxyribonucleic acid (DNA) to ensure high selectivity. To provide better or more convenient biological immobilization methods for researchers in this field and thereby improve detection sensitivity, this review summarizes recent developments in the immobilization strategies used for biological macromolecules in OFETs, including cross-linking, physical adsorption, embedding, and chemical covalent binding. The influences of biomolecules on device performance are also discussed.

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“…Organic field‐effect transistor (OFET)‐based sensors are currently considered for various applications such as gas sensing, biosensing, and pressure sensing . OFET‐based biosensors are being widely studied for potentially fast clinical diagnostics, with the advantages of flexibility, sensitivity, and low cost . Particularly, OFET‐based biosensors for protein sensing have progressed remarkably for label‐free analyte detection based on the specific immune binding property innate to particular antibodies .…”

Section: Introductionmentioning

confidence: 99%

Influence of Bioreceptor Layer Structure on Myelin Basic Protein Detection using Organic Field Effect Transistor‐Based Biosensors

Song

Dailey

et al. 2018

Adv Funct Materials

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Organic field-effect transistor (OFET)-based biosensors with antibody receptors are widely considered for protein antigen detection. To the authors' knowledge, there are no comparative evaluations to date of different choices for the matrix polymer to which the antibodies are attached. Herein, multiple acrylic copolymers are studied as receptor layers with myelin basic protein and its corresponding antibody as an archetypal antibodyantigen pair. Stability against multiple washing steps and the capacity for immobilizing antibodies on polymers on device surfaces with the help of fluorescein isothiocyanate-labeled antibodies are compared. Electronic detection and selectivity are also observed. The conclusions provide guidance on the selection of bioreceptor material for increasing sensitivity and process stability of OFET biosensors.

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The hybridization and optimization of complementary DNA molecules on organic field-effect transistors

Cited by 12 publications

References 20 publications

P and N type copper phthalocyanines as effective semiconductors in organic thin-film transistor based DNA biosensors at elevated temperatures

P and N type copper phthalocyanines as effective semiconductors in organic thin-film transistor based DNA biosensors at elevated temperatures

Recent Advances in Immobilization Strategies for Biomolecules in Sensors Using Organic Field-Effect Transistors

Influence of Bioreceptor Layer Structure on Myelin Basic Protein Detection using Organic Field Effect Transistor‐Based Biosensors

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