Label-free detection of protein-protein interactions on multi-scale micro-well arrays using spatial light modulator

In general, lower viscosity of materials leads to desirable nano‐pattern replication characteristics in injection molding processes. However, such materials do not meet the properties required for manufacturable products owing to their poor mechanical properties. In this study, we blended a small amount of dendrimer with high‐viscosity polyamide 6 to lower viscosity and enhance flowability of the polymer blend while maintaining desirable mechanical properties. It was observed that the flowability of the polyamide 6/dendrimer blend increased significantly with an increase in dendrimer content without affecting the mechanical properties of high‐viscosity polyamide 6. To investigate the nano‐pattern replication characteristics of the blend, injection molding process and the mold of metal stamp were used to manufacture a nano‐pattern specimen. Blending more than 0.6 wt% of dendrimer with high‐viscosity polyamide 6 significantly improved its nano‐pattern replication characteristics and flowability.

show abstract

“…Schematic presentation of the metal stamp manufacturing process 33, 34 [Color figure can be viewed at wileyonlinelibrary.com]…”

Section: Methodsmentioning

confidence: 99%

Improvement in nano‐pattern replication of injection molding by polyamide/dendrimer blend

Kang

Kim

2021

Polymer Engineering & Sci

View full text Add to dashboard Cite

show abstract

“…Recently, a couple of reports on parallelized label-free detection with multiple array chips have been published, thus reecting the urgent need for the technology. [17][18][19][20][21][22][23][24][25] In this paper, we performed the multiple detection of antigen-antibody interactions with Au-capped nanopillar chips fabricated by the nanoimprint technique and a hyperspectral imaging system.…”

Section: Introductionmentioning

confidence: 99%

Parallelized label-free detection of protein interactions using a hyper-spectral imaging system

et al. 2015

View full text Add to dashboard Cite

We describe parallelized label-free detection of protein interactions using a hyper-spectral imaging system. Multi-array protein chips were fabricated by immobilizing antibodies on Au-capped nanopillar polymer films, which showed an optical absorption band due to the localised surface plasmon resonance (LSPR) in the absorption spectrum. Parallelized detection of multiple antigen-antibody interactions was demonstrated by processing hyper-spectral images of the multi-array chip before and after the application of antigens. Human immunoglobulin A (IgA) and human C-reactive protein (CRP) were specifically detected, and the signal corresponding to these protein concentrations was obtained, indicating that this technique is promising for use in enhancing the performance of multiple protein assays.

show abstract

Label-free detection of protein-protein interactions on multi-scale micro-well arrays using spatial light modulator

Abstract: Articles you may be interested inEnhancement of a whispering gallery mode microtoroid resonator by plasmonic triangular gold nanoprism for label-free biosensor applications

Cited by 2 publications

References 18 publications

Improvement in nano‐pattern replication of injection molding by polyamide/dendrimer blend

Improvement in nano‐pattern replication of injection molding by polyamide/dendrimer blend

Parallelized label-free detection of protein interactions using a hyper-spectral imaging system

Contact Info

Product

Resources

About