Label-free determination of prostate specific membrane antigen in human whole blood at nanomolar levels by magnetically assisted surface enhanced Raman spectroscopy

Chaloupková, Zuzana; Balzerová, Anna; Bařinková, Jitka; Medříková, Zdenka; Šácha, Pavel; Beneš, P.; Ranc, Václav; Konvalinka, Jan; Zbořil, Radek

doi:10.1016/j.aca.2017.10.008

Cited by 18 publications

(10 citation statements)

References 38 publications

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“…Several SERS platforms have been reported for early-stage prostate cancer diagnosis. Some of the more researched morphologies include MNP-AuNP (Figure 11a) [233] and MNP-AgNP assemblies [234]. SERS-based genetic assays have been reported to be four orders of magnitude more sensitive than polymerase chain reaction (PCR) assays [235].…”

Section: Sers Biosensorsmentioning

confidence: 99%

Recent Progress in Optical Sensors for Biomedical Diagnostics

2020

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In recent years, several types of optical sensors have been probed for their aptitude in healthcare biosensing, making their applications in biomedical diagnostics a rapidly evolving subject. Optical sensors show versatility amongst different receptor types and even permit the integration of different detection mechanisms. Such conjugated sensing platforms facilitate the exploitation of their neoteric synergistic characteristics for sensor fabrication. This paper covers nearly 250 research articles since 2016 representing the emerging interest in rapid, reproducible and ultrasensitive assays in clinical analysis. Therefore, we present an elaborate review of biomedical diagnostics with the help of optical sensors working on varied principles such as surface plasmon resonance, localised surface plasmon resonance, evanescent wave fluorescence, bioluminescence and several others. These sensors are capable of investigating toxins, proteins, pathogens, disease biomarkers and whole cells in varied sensing media ranging from water to buffer to more complex environments such as serum, blood or urine. Hence, the recent trends discussed in this review hold enormous potential for the widespread use of optical sensors in early-stage disease prediction and point-of-care testing devices.

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Section: Sers Biosensorsmentioning

confidence: 99%

Recent Progress in Optical Sensors for Biomedical Diagnostics

2020

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“…Prostate cancer is the most prevalent type of cancer disease and it causes death to almost 12% of all cancer cases. [ 1,2 ] For early diagnosis of prostate cancer, measurement of the blood concentration of prostate specific antigen (PSA) and digital rectal exam are the usually utilized prostate cancer diagnosis methods. [ 2,3 ] Nevertheless, the main obstruction of PSA analysis is its low specificity that causes a high negative biopsy rate.…”

Section: Introductionmentioning

confidence: 99%

“…[ 1,2 ] For early diagnosis of prostate cancer, measurement of the blood concentration of prostate specific antigen (PSA) and digital rectal exam are the usually utilized prostate cancer diagnosis methods. [ 2,3 ] Nevertheless, the main obstruction of PSA analysis is its low specificity that causes a high negative biopsy rate. Furthermore, PSA is not a specific marker of prostate cancer because with some conditions such as benign prostatic hyperplasia and non‐specific inflammation the PSA level is high.…”

Section: Introductionmentioning

confidence: 99%

“…[ 11 ] It catalyzes the hydrolysis reaction of N ‐acetyl aspartyl glutamate to glutamate and it is synthesized at high concentrations in prostate cancer cells. [ 2,12 ] Furthermore, it is synthesized at low concentrations in benign prostatic epithelium. [ 12 ] The concentrations of PSMA in prostate cell is associated to the aggressiveness of tumor growth.…”

Section: Introductionmentioning

confidence: 99%

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Ultrasensitive and Selective Impedimetric Determination of Prostate Specific Membrane Antigen Based on Di‐Succinimide Functionalized Polythiophene Covered Cost‐Effective Indium Tin Oxide

Aydın

Sezgintürk

2021

Macromolecular Bioscience

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A new and ultrasensitive impedimetric biosensor fabricated by using conjugated di‐succinimide substituted polythiophene (P(ThidiSuc)) polymer modified indium tin oxide electrode is developed for the first time to detect the prostate specific membrane antigen (PSMA). The polymer P(Thi‐diSuc) is synthesized by using a simple way and used in the fabrication of the proposed biosensor. The synthesized polymer contains di‐succinimide groups, which offers covalent immobilization of PSMA specific antibodies. The developed strategy shortens the biosensor fabrication steps, because these active groups bind covalently to the amino ends of PSMA specific antibodies and this reaction does not require any crosslinking agent. Various characterization studies like impedimetric and voltammetric measurements, and morphological analyses are utilized to confirm the successful development of the biosensor. Under optimum conditions, the biosensing ability of the PSMA determination has a wide linear determination range from 0.015 to 14.4 pg mL−1, as well as a low limit of detection of 6.4 fg mL−1 and a high sensitivity of 1.36 kohm pg−1 mL cm−2. Furthermore, the proposed biosensor is able to measure the PSMA antigen in real human serums, which offers that it is a simple, low‐cost, and sensitive tool with excellent potential for application in the quantification of PSMA.

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“…Until now, several analytical techniques have been developed for determination of PSA and Myo individually. These include chromatography [8,9], mass spectroscopy [10,11], fluorescence spectroscopy [12,13], nuclear magnetic resonance spectroscopy [14,15], white light reflectance spectroscopy [16,17], capillary electrophoresis [18], chemiluminescence [19], enzyme-linked immunosorbent assays (ELISA) [20,21], electrochemiluminescence [22,23], radioimmunoassay [24,25], time-resolved immunofluorometric assay [26], surface plasmon fluorescence immunoassay [27], bioluminescent immunoassay [28], electrochemical [29], surface-enhanced Raman scattering [24,30] and microcantilever method [31]. Of these methods, biosensors especially electrochemical types can offer some advantages in contrast to the commonly used sensing tools, including simultaneous analysis of biomarkers, capability of miniaturization, lower cost and simplicity of analysis [32][33][34].…”

Section: Introductionmentioning

confidence: 99%

Dual-modality impedimetric immunosensor for early detection of prostate-specific antigen and myoglobin markers based on antibody-molecularly imprinted polymer

Karami¹,

Bagheri

Johari-Ahar

et al. 2019

Talanta

120

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A new dual-modality immunosensor based on molecularly imprinted polymer (MIP) and a nanostructured biosensing layer has fabricated for the simultaneous detection of two important markers including prostatespecific antigen (PSA) and myoglobin (Myo) in human serum and urine samples. In the first step, 3,3′-dithiodipropionic acid di(N-hydroxysuccinimide ester) (DSP) was self-assembled on a gold screen printed electrode (SPE). Then, the target proteins were attached covalently to the DSP-SPE. The imprinted cocktail polymer ((MIP (PSA, Myo)-SPE)) was synthesized at the SPE surface using acrylamide as monomer, N,N′-methylenebisacrylamide as a crosslinker, and PSA and Myo as the templates, respectively. The MIP-SPE was specific for the impedimetric sensing of PSA and Myo. After that, a nanocomposite (NCP) was synthesized based on the decorated magnetite nanoparticles with multi-walled carbon nanotube, graphene oxide and specific antibody for PSA (Ab). Then, NCP incubated with (MIP(PSA, Myo)-SPE. The modified electrodes and synthesized nanoparticles were characterized using electrochemical impedance spectroscopy, dynamic light scattering, surface plasmon resonance and scanning electron microscopy. The limits of detections were found to be 5.4 pg mL −1 and 0.83 ng mL −1 with the linear dynamic ranges of 0.01-100 and 1-20000 ng mL −1 for PSA and Myo, respectively. The ability of proposed biosensor to detect PSA and Myo simultaneously with high sensitivity and specificity offers a powerful opportunity for the new generation of biosensors. This dual-analyte specific receptors-based device is highly desired for the integration with lab-on-chip kits to measure a wide panel of biomarkers present at ultralow levels during early stages of diseases progress.

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Label-free determination of prostate specific membrane antigen in human whole blood at nanomolar levels by magnetically assisted surface enhanced Raman spectroscopy

Cited by 18 publications

References 38 publications

Recent Progress in Optical Sensors for Biomedical Diagnostics

Recent Progress in Optical Sensors for Biomedical Diagnostics

Ultrasensitive and Selective Impedimetric Determination of Prostate Specific Membrane Antigen Based on Di‐Succinimide Functionalized Polythiophene Covered Cost‐Effective Indium Tin Oxide

Dual-modality impedimetric immunosensor for early detection of prostate-specific antigen and myoglobin markers based on antibody-molecularly imprinted polymer

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