Ultrasensitive and Selective Impedimetric Determination of Prostate Specific Membrane Antigen Based on Di‐Succinimide Functionalized Polythiophene Covered Cost‐Effective Indium Tin Oxide

Aydın, Elif Burcu; Aydın, Muhammet; Sezgintürk, Mustafa Kemal

doi:10.1002/mabi.202100173

Cited by 6 publications

(6 citation statements)

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“…These electrochemical variations are detectable by following the R ct that mostly rise with the amount in the quantity of target analytes attached onto the working electrode surface. [26][27][28][29] This study demonstrated the fabrication of EIS-based immunosensor for quantification of CALR in human serum samples. For designing of the immunosensor, a nanocomposite layer composed of amino substituted polypyrrole polymer (PPyNH 2 ) and rGO was coated on disposable and low-cost ITO electrode.…”

Section: Introductionmentioning

confidence: 83%

A Simple and Low‐Cost Electrochemical Immunosensor for Ultrasensitive Determination of Calreticulin Biomarker in Human Serum

Aydın

Sezgintürk

2022

Macromolecular Bioscience

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An early on time detection of breast cancer significantly affects the treatment process and outcome. Herein, a new label‐free impedimetric biosensor is developed to determine the lowest change in the level of calreticulin (CALR), which is a new biomarker of breast carcinoma. The proposed immunosensor is fabricated by using reduced graphene oxide/amino substituted polypyrrole polymer (rGO‐PPyNH2) nanocomposite modified disposable electrode. The anti‐CALR antibodies are first attached on the rGO‐PPyNH2 nanocomposite coated electrode through glutaraldehyde crosslinking; the CALR antigens are then immobilized with the addition of CALR antigens to form an immunocomplex on the sensing surface. This immunocomplex induces considerably larger interfacial electron transport resistance (Rct). The variation in the Rct has a linear relationship with CALR level in the detection range of 0.025 to 75 pg mL−1, with a detection limit of 10.4 fg mL−1. The suggested biosensor shows high selectivity to CALR, good storage stability (at least 5 weeks) and suitable reproducibility results as shown in quality control chart. The designed immunosensor is utilized to analyze CALR levels in human sera with satisfying results. This immunosensor provides a novel way for the clinical determination of CALR and other cancer biological markers.

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Section: Introductionmentioning

confidence: 83%

A Simple and Low‐Cost Electrochemical Immunosensor for Ultrasensitive Determination of Calreticulin Biomarker in Human Serum

Aydın

Sezgintürk

2022

Macromolecular Bioscience

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“…Aydın et al (Figure 21) reported a novel and ultrasensitive impedimetric biosensor fabricated using conjugated di-succinimide substituted polythiophene (P(ThidiSuc)) polymer modified indium tin oxide electrode to detect the PSMA. [93] Studies have shown that PSMA is expressed in all types of prostate tissue and that expression is increased in cancerous tissues. [93] The synthesized polymer (P(ThidiSuc)) contains di-succinimide groups, which offered covalent immobilization of PSMA specific antibodies and did not require any cross-linking agent.…”

Section: Other Biomarkers-based Biosensors (Including Dual-biomarkers...mentioning

confidence: 99%

“…[93] Studies have shown that PSMA is expressed in all types of prostate tissue and that expression is increased in cancerous tissues. [93] The synthesized polymer (P(ThidiSuc)) contains di-succinimide groups, which offered covalent immobilization of PSMA specific antibodies and did not require any cross-linking agent. Under optimum conditions, a wide linear range (0.015 to14.4 pg mL −1 ), and an LOD of 6.4 fg mL −1 were achieved.…”

Section: Other Biomarkers-based Biosensors (Including Dual-biomarkers...mentioning

confidence: 99%

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Nanomaterials‐Based Biosensors for the Detection of Prostate Cancer Biomarkers: Recent Trends and Future Perspective

Singh

Hara³

et al. 2023

Adv Materials Technologies

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Cancer is among the leading causes of death and an important barrier to improving life expectancy globally. Prostate cancer is the second most common cancer in men worldwide. The detection of biomarkers in body fluids is the key topic for the diagnosis and prognosis of prostate cancer. Despite advances in prostate cancer detection methods, therapeutic agents and new biomarkers, prostate cancer remains a serious challenge. Prostate‐specific antigen (PSA) is widely recognized as an important biomarker for the diagnosis of prostate cancer although researchers have also investigated the use of alternative biomarkers. Design and development of novel biosensors for prostate cancer detection has become a hot research area with advances in nanotechnology aiding biosensor development. This article reviews recent achievements and progress that nanomaterials and nanotechnology have made in biomarkers based biosensors for prostate cancer detection and covers: i) PSA‐targeted biosensors (immunosensors, aptamer‐based, peptide‐based and nanopore‐based biosensors), ii) sarcosine oxidase‐targeted biosensors, iii) other biomarkers based biosensors (prostate cancer antigen 3 (PCA3), vascular endothelial growth factor (VEGF) and prostate‐specific membrane antigen (PSMA)) including dual biomarkers based biosensors (PSA‐VEGF, PSA‐PSMA, PSA‐PCA3 and PSA‐sarcosine). The aim of this review is to provide insights into how nanomaterials in combination with various biomarkers are now aiding biosensor development in prostate cancer diagnostics.

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“…The biocompatibility property of polypyrrole makes it a proper immobilization matrix material for binding of biorecognition molecules. Furthermore, polypyrrole can be synthesized with electrochemical polymerization on the electrode surface. , Polypyrrole, a particular conducting polymer with a high application potential, is a quite suitable candidate for the synthesis of nanotube composites and appropriate for being an immobilization matrix material . In a previous study, a polyaniline-SWCNT nanocomposite was synthesized on the working electrode, and the prepared electrode was utilized for detection of triglyceride .…”

Section: Introductionmentioning

confidence: 99%

Impedimetric Detection of Calreticulin by a Disposable Immunosensor Modified with a Single-Walled Carbon Nanotube-Conducting Polymer Nanocomposite

Aydın

Sezgintürk

2022

ACS Biomater. Sci. Eng.

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A label-free impedimetric immunosensing system was constructed for ultrasensitive determination of the calreticulin (CALR) biological marker in human serum samples utilizing an electrochemical impedance spectroscopy analysis technique for the first time. The new biosensor fabrication procedure consisted of electrodeposition of single-walled carbon nanotubes (SWCNTs) incorporating polymerization of an oxiran-2-yl methyl 3-(1H-pyrrol-1-yl) propanoate monomer (Pepx) onto a lowcost and disposable indium tin oxide (ITO) electrode. The SWCNTs-PPepx nanocomposite layer was prepared onto the ITO after the one-step fabrication procedure. The fabrication procedure of the immunosensor and the characteristic biomolecular interactions between the anti-CALR and CALR were characterized by electrochemical analysis and morphological monitoring techniques. Under optimum conditions, the proposed biosensor was responsive to CALR concentrations over the detection ranges of 0.015−60 pg/mL linearly, and it had a very low detection limit (4.6 fg/mL) and a favorable sensitivity (0.43 kΩ pg −1 mL cm −2 ). The reliability of the biosensor system in clinical analysis was investigated by successful quantification of CALR levels in human serum. Moreover, the repeatability and reproducibility results of the biosensor were evaluated by using Dixon, Grubbs, T-test, and F-tests. Consequently, the proposed biosensor was a promising method for scientific, rapid, and successful analysis of CALR in human serum samples.

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Ultrasensitive and Selective Impedimetric Determination of Prostate Specific Membrane Antigen Based on Di‐Succinimide Functionalized Polythiophene Covered Cost‐Effective Indium Tin Oxide

Cited by 6 publications

References 60 publications

A Simple and Low‐Cost Electrochemical Immunosensor for Ultrasensitive Determination of Calreticulin Biomarker in Human Serum

A Simple and Low‐Cost Electrochemical Immunosensor for Ultrasensitive Determination of Calreticulin Biomarker in Human Serum

Nanomaterials‐Based Biosensors for the Detection of Prostate Cancer Biomarkers: Recent Trends and Future Perspective

Impedimetric Detection of Calreticulin by a Disposable Immunosensor Modified with a Single-Walled Carbon Nanotube-Conducting Polymer Nanocomposite

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