Label‐Free Direct Electrical Detection of a Histidine‐Rich Protein with Sub‐Femtomolar Sensitivity using an Organic Field‐Effect Transistor

Minamiki, Tsukuru; Sasaki, Yoko; Tokito, Shizuo; Minami, Tsuyoshi

doi:10.1002/open.201700070

Cited by 40 publications

(33 citation statements)

References 64 publications

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“…The organic semiconductor layer (OSC) made of poly(2,5‐bis(3‐hexadecylthiophene‐2‐yl)thieno[3,2‐ b ]thiophene) (PBTTT) was formed by a drop‐casting method and was baked at 160 °C for 10 min. PBTTT materials have been widely applied to polymer FETs because of their excellent carrier mobility, solubility for organic solvents, and performance uniformity and stability . Hence, PBTTT is one of the most suitable materials for the development of OFET‐based sensors .…”

Section: Figurementioning

confidence: 99%

“…[39,40,41] Hence, PBTTT is one of the most suitable materials for the development of OFET-based sensors. [41] Finally, a Cytop passivation layer (CTL-809M in CT-Solv.180, ratio 1 : 1 (v/v)) was spin-coated on the OSC layer and baked at 110°C for 10 min. The extended-gate component (the sensing area) (15 mm 2 ) was fabricated by thermal vacuum deposition of gold (50 nm in thickness).…”

Section: Microfluidic System With Extended-gate-type Organic Transistmentioning

confidence: 99%

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Microfluidic System with Extended‐Gate‐Type Organic Transistor for Real‐Time Glucose Monitoring

et al. 2020

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Organic field‐effect transistors (OFETs) can be potentially employed to monitor cell activities for healthcare and medical treatment because of their attractive properties such as ease of use, flexibility, and low‐cost manufacturing processes. Although current OFET‐based sensors are suitable for point‐of‐care testing, the establishment of real‐time monitoring methods is in high demand for continuous monitoring of health conditions and/or biological cell activities. In this regard, we herein propose a microfluidic platform integrated with an extended‐gate‐type OFET for real‐time glucose monitoring. The mechanism of glucose detection depends on the artificial receptor phenylboronic acid and its boronate esterification. After optimization of the microfluidics for the OFET‐based sensor, the sensor was used to monitor glucose consumption and release in a model of pseudo‐liver cells. Random increases or decreases in the glucose concentration were reproducibly monitored.

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Section: Figurementioning

confidence: 99%

Section: Microfluidic System With Extended-gate-type Organic Transistmentioning

confidence: 99%

Microfluidic System with Extended‐Gate‐Type Organic Transistor for Real‐Time Glucose Monitoring

et al. 2020

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“…Therefore, there is still room for the further development of biosensing devices by using other transduction mechanisms. However, we already proposed the bottom-up [100] or top-down approaches [101] for the amplification of the analyte information in the electrical device-based sensors. Hence, although various transducing mechanisms can be utilized for the building of molecular assembly-functionalized chemical/biosensors, we believe that electrical devices are one of the key platforms for the preparation of sensing systems for the on-site analyses of required target species owing to their quantitativity, easy fabrication, and compact integration.…”

Section: Conclusion and Future Perspectivesmentioning

confidence: 99%

The Power of Assemblies at Interfaces: Nanosensor Platforms Based on Synthetic Receptor Membranes

Minamiki

Ichikawa

Kurita

2020

Sensors

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Synthetic sensing materials (artificial receptors) are some of the most attractive components of chemical/biosensors because of their long-term stability and low cost of production. However, the strategy for the practical design of these materials toward specific molecular recognition in water is not established yet. For the construction of artificial material-based chemical/biosensors, the bottom-up assembly of these materials is one of the effective methods. This is because the driving forces of molecular recognition on the receptors could be enhanced by the integration of such kinds of materials at the 'interfaces', such as the boundary portion between the liquid and solid phases. Additionally, the molecular assembly of such self-assembled monolayers (SAMs) can easily be installed in transducer devices. Thus, we believe that nanosensor platforms that consist of synthetic receptor membranes on the transducer surfaces can be applied to powerful tools for high-throughput analyses of the required targets. In this review, we briefly summarize a comprehensive overview that includes the preparation techniques for molecular assemblies, the characterization methods of the interfaces, and a few examples of receptor assembly-based chemical/biosensing platforms on each transduction mechanism.Synthetic receptors (i.e., artificial molecular recognition materials) are some of the most suitable platform materials for the development of chemical/biosensors owing to their chemical/physical stability, low cost of production, and their fine-tuning ability to select the required targets [4]. However, the preparation of artificial receptor-based sensors for practical applications is still in its initial stages, since the analyte specificity of most of the artificial materials is generally lower than that of biomaterials (i.e., antibodies and enzymes) [5]. To improve the binding affinity between the artificial receptors and the analytes, complicated synthesis procedures are required. Hence, a simpler way to improve the sensing ability of the artificial receptors should be considered to bring out the attractive features of these materials. To facilitate this, bottom-up integration of the receptors as molecular assemblies is considered as one of the most useful approaches for the construction of selective sensing fields for analytes. Moreover, the sensing features in the artificial receptor-based sensors could be finely tuned by using top-down technologies (e.g., molecular imprinting techniques [6], etc.) because the molecular recognition ability of the receptor assemblies depends on their nanostructures [7]. As aforementioned, the molecular assembly enhances the functions of a single kind of molecule ( Figure 1) [8]. In addition, the driving forces in molecular recognition (i.e., noncovalent interactions) can be amplified at the interfaces such as the boundary portion between the liquid and solid phases [9,10]. Thus, we believe that the installation of artificial receptors at the surface of the sensing portion in selective transducer...

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“…OFET‐based biosensors are being widely studied for potentially fast clinical diagnostics, with the advantages of flexibility, sensitivity, and low cost . Particularly, OFET‐based biosensors for protein sensing have progressed remarkably for label‐free analyte detection based on the specific immune binding property innate to particular antibodies . By the adjustment of device structures, various challenges have been successfully overcome.…”

Section: Introductionmentioning

confidence: 99%

“…As an example, organic materials are extremely sensitive to humidity, which have been a great drawback of OFET‐based biosensors. Minami and co‐workers reported an on‐site testing of histidine‐rich protein by using an extended gate device structure, which completely separates the sensing area from the semiconductor layer . Debye screening length, beyond which voltage changes are not sensed in a solution, is also a great challenge for OFET‐based biosensors.…”

Section: Introductionmentioning

confidence: 99%

Influence of Bioreceptor Layer Structure on Myelin Basic Protein Detection using Organic Field Effect Transistor‐Based Biosensors

Song

Dailey

et al. 2018

Adv Funct Materials

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Organic field-effect transistor (OFET)-based biosensors with antibody receptors are widely considered for protein antigen detection. To the authors' knowledge, there are no comparative evaluations to date of different choices for the matrix polymer to which the antibodies are attached. Herein, multiple acrylic copolymers are studied as receptor layers with myelin basic protein and its corresponding antibody as an archetypal antibodyantigen pair. Stability against multiple washing steps and the capacity for immobilizing antibodies on polymers on device surfaces with the help of fluorescein isothiocyanate-labeled antibodies are compared. Electronic detection and selectivity are also observed. The conclusions provide guidance on the selection of bioreceptor material for increasing sensitivity and process stability of OFET biosensors.

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Label‐Free Direct Electrical Detection of a Histidine‐Rich Protein with Sub‐Femtomolar Sensitivity using an Organic Field‐Effect Transistor

Cited by 40 publications

References 64 publications

Microfluidic System with Extended‐Gate‐Type Organic Transistor for Real‐Time Glucose Monitoring

Microfluidic System with Extended‐Gate‐Type Organic Transistor for Real‐Time Glucose Monitoring

The Power of Assemblies at Interfaces: Nanosensor Platforms Based on Synthetic Receptor Membranes

Influence of Bioreceptor Layer Structure on Myelin Basic Protein Detection using Organic Field Effect Transistor‐Based Biosensors

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