Label-free electrochemical detection of neuraminidase activity: A facile whole blood diagnostic probe for infectious diseases

Mubarok, Ahmad Zaki; Mani, Veerappan; Chang, Pu-Chieh; Huang, Sheng‐Tung

doi:10.1016/j.snb.2017.06.061

Cited by 15 publications

(9 citation statements)

References 45 publications

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“…Several methods can show better characteristics in limit of detection (LOD) [11] or detection time [8] but are inferior in protection against non-specific binding or lifetime stability because antibodies are used. Using linear sweep voltammetry [44] for neuraminidase detection shows good stability and applicability but has poor sensitivity and selectivity due to association of NA with some bacterial infections.…”

Section: Resultsmentioning

confidence: 99%

Label-free sensitive detection of influenza virus using PZT discs with a synthetic sialylglycopolymer receptor layer

Erofeev

Gorelkin²,

Колесов

et al. 2019

R. Soc. open sci.

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We describe rapid, label-free detection of Influenza A viruses using the first radial mode of oscillations of lead zirconate titanate (PZT) piezoelectric discs with a 2 mm radius and 100 µm thickness fabricated from a piezoelectric membrane. The discs are modified with a synthetic sialylglycopolymer receptor layer, and the coated discs are inserted in a flowing virus suspension. Label-free detection of the virus is achieved by monitoring the disc radial mode resonance frequency shift. Piezo transducers with sialylglycopolymer sensor layers exhibited a long lifetime, a high sensitivity and the possibility of regeneration. We demonstrate positive, label-free detection of Influenza A viruses at concentrations below 10 5 virus particles per millilitre. We show that label-free, selective, sensitive detection of influenza viruses by home appliances is possible in principle.

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Section: Resultsmentioning

confidence: 99%

Label-free sensitive detection of influenza virus using PZT discs with a synthetic sialylglycopolymer receptor layer

Erofeev

Gorelkin²,

Колесов

et al. 2019

R. Soc. open sci.

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“…Interesting research of Mubarok et al [106] showed whole blood analysis for neuraminidase activity label-free detection. The cleavage of glycosidic linkage of self-synthetized N -acetyl-2- O -(4- aminophenyl)-α-neuraminic acid (AP-Neu5Ac) in the presence of NA released p-aminophenol molecules.…”

Section: Novel Detection Methods Of Influenza Virusmentioning

confidence: 99%

Detection Methods of Human and Animal Influenza Virus—Current Trends

Dziąbowska¹,

Czaczyk²,

Nidzworski³

2018

Biosensors

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The basic affairs connected to the influenza virus were reviewed in the article, highlighting the newest trends in its diagnostic methods. Awareness of the threat of influenza arises from its ability to spread and cause a pandemic. The undiagnosed and untreated viral infection can have a fatal effect on humans. Thus, the early detection seems pivotal for an accurate treatment, when vaccines and other contemporary prevention methods are not faultless. Public health is being attacked with influenza containing new genes from a genetic assortment between animals and humankind. Unfortunately, the population does not have immunity for mutant genes and is attacked in every viral outbreak season. For these reasons, fast and accurate devices are in high demand. As currently used methods like Rapid Influenza Diagnostic Tests lack specificity, time and cost-savings, new methods are being developed. In the article, various novel detection methods, such as electrical and optical were compared. Different viral elements used as detection targets and analysis parameters, such as sensitivity and specificity, were presented and discussed.

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“…Electrochemical nanobiosensors have been applied in several fields [ 25 , 26 , 29 , 57 , 106 , 107 ]. An electrochemical nanobiosensor is a molecular sensing device that couples a biological recognition event with an electrode transducer to produce a useful electrical signal [ 108 ]. Electrochemical nanosensors contain electrodes, for which the semiconductors properties, dielectric properties, and charge distribution are important factors.…”

Section: Nanobiosensors For the Detection Of Human Coronavirus (20mentioning

confidence: 99%

“…Bioreceptors must be integrated effectively into the right transducer to detect the presence of any virus within the target sample. For the detection of different types of coronaviruses, most researchers used electrochemical detection methods, such as field effect transistor (FET) [ 69 , 110 , 111 ], bioelectric recognition assay (BERA) [ 111 ], electrochemical impedance spectroscopy (EIS) [ 78 , 112 , 113 , 114 , 115 , 116 , 117 ], amperometry [ 27 , 118 , 119 ], cyclic voltammetry [ 53 , 62 , 87 , 108 , 120 , 121 , 122 ], or conductometry [ 55 , 123 ]. Influenza is an acute infectious disease caused by a coated ribonucleic acid (RNA), which contains viruses belonging to the Orthomyxoviridae family [ 80 ].…”

Section: Nanobiosensors For the Detection Of Human Coronavirus (20mentioning

confidence: 99%

Nanobiosensors for the Detection of Novel Coronavirus 2019-nCoV and Other Pandemic/Epidemic Respiratory Viruses: A Review

Alhalaili

Popescu

Kamoun

et al. 2020

Sensors

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The coronavirus disease 2019 (COVID-19) pandemic is considered a public health emergency of international concern. The 2019 novel coronavirus (2019-nCoV) or severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) that caused this pandemic has spread rapidly to over 200 countries, and has drastically affected public health and the economies of states at unprecedented levels. In this context, efforts around the world are focusing on solving this problem in several directions of research, by: (i) exploring the origin and evolution of the phylogeny of the SARS-CoV-2 viral genome; (ii) developing nanobiosensors that could be highly effective in detecting the new coronavirus; (iii) finding effective treatments for COVID-19; and (iv) working on vaccine development. In this paper, an overview of the progress made in the development of nanobiosensors for the detection of human coronaviruses (SARS-CoV, SARS-CoV-2, and Middle East respiratory syndrome coronavirus (MERS-CoV) is presented, along with specific techniques for modifying the surface of nanobiosensors. The newest detection methods of the influenza virus responsible for acute respiratory syndrome were compared with conventional methods, highlighting the newest trends in diagnostics, applications, and challenges of SARS-CoV-2 (COVID-19 causative virus) nanobiosensors.

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Label-free electrochemical detection of neuraminidase activity: A facile whole blood diagnostic probe for infectious diseases

Cited by 15 publications

References 45 publications

Label-free sensitive detection of influenza virus using PZT discs with a synthetic sialylglycopolymer receptor layer

Label-free sensitive detection of influenza virus using PZT discs with a synthetic sialylglycopolymer receptor layer

Detection Methods of Human and Animal Influenza Virus—Current Trends

Nanobiosensors for the Detection of Novel Coronavirus 2019-nCoV and Other Pandemic/Epidemic Respiratory Viruses: A Review

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