Label-free Electrochemical Detection of the Human Adenovirus 40/41 Fiber Gene

Song, Mee; Jin, Joon-Hyung

doi:10.2116/analsci.31.159

Cited by 8 publications

(3 citation statements)

References 34 publications

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“…Recently, several types of biosensing strategies, especially electrochemical versions, have been evaluated for diagnosing different serotypes of adenoviruses in clinical samples, while there is only one published for enteric adenovirus 40 and 41 [ 160 , 161 ]. There is a label-free nucleic-acid-based electrochemical sensor that was developed by Song et al for diagnosing the human adenovirus 40/41 fiber gene [ 162 ]. To construct this sensor, polypyrrole (PPy) film was electropolymerized on nanoporous silicon (NPS), and then a 25 bp DNA probe (PDNA), which targets fiber genes, was fixed on the PPy-coated NPS.…”

Section: Enteric Adenovirus 40 and 41mentioning

confidence: 99%

Recent Advances in Early Diagnosis of Viruses Associated with Gastroenteritis by Biosensors

et al. 2022

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Gastroenteritis, as one of the main worldwide health challenges, especially in children, leads to 3–6 million deaths annually and causes nearly 20% of the total deaths of children aged ˂5 years, of which ~1.5 million gastroenteritis deaths occur in developing nations. Viruses are the main causative agent (~70%) of gastroenteritis episodes and their specific and early diagnosis via laboratory assays is very helpful for having successful antiviral therapy and reduction in infection burden. Regarding this importance, the present literature is the first review of updated improvements in the employing of different types of biosensors such as electrochemical, optical, and piezoelectric for sensitive, simple, cheap, rapid, and specific diagnosis of human gastroenteritis viruses. The Introduction section is a general discussion about the importance of viral gastroenteritis, types of viruses that cause gastroenteritis, and reasons for the combination of conventional diagnostic tests with biosensors for fast detection of viruses associated with gastroenteritis. Following the current laboratory detection tests for human gastroenteritis viruses and their limitations (with subsections: Electron Microscope (EM), Cell Culture, Immunoassay, and Molecular Techniques), structural features and significant aspects of various biosensing methods are discussed in the Biosensor section. In the next sections, basic information on viruses causing gastroenteritis and recent developments for fabrication and testing of different biosensors for each virus detection are covered, and the prospect of future developments in designing different biosensing platforms for gastroenteritis virus detection is discussed in the Conclusion and Future Directions section as well.

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Section: Enteric Adenovirus 40 and 41mentioning

confidence: 99%

Recent Advances in Early Diagnosis of Viruses Associated with Gastroenteritis by Biosensors

et al. 2022

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“…Here, the signal transduction can be classified into direct assay and indirect detection approaches. In the direct assay, the specific recognition of target molecules by the probe without label can be detected by the changing electrical properties of the conducting polymer composites directly through amperometry (Ionescu et al, 2006), coulometry (Song and Jin, 2015), voltammetry (Galán et al, 2015) and impedimetry (Tran et al, 2014). For instance, Galán et al (Galán et al, 2015) developed a label-free electrochemical DNA sensor for a specific "Hepatitis C" virus sequence based on azido-derivatised PEDOT modified electrode.…”

Section: Conducting Polymers (Cps)mentioning

confidence: 99%

Soft and flexible material-based affinity sensors

Meng

Turner

Mak

2020

Biotechnology Advances

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Recent advances in biosensors and point-of-care (PoC) devices are poised to change and expand the delivery of diagnostics from conventional lateral-flow assays and test strips that dominate the market currently, to newly emerging wearable and implantable devices that can provide continuous monitoring. Soft and flexible materials are playing a key role in propelling these trends towards real-time and remote health monitoring. Affinity biosensors have the capability to provide for diagnosis and monitoring of cancerous, cardiovascular, infectious and genetic diseases by the detection of biomarkers using affinity interactions. This review tracks the evolution of affinity sensors from conventional lateral-flow assay and test strips to wearable/implantable devices enabled by soft and flexible materials. Initially, we highlight conventional affinity sensors exploiting membrane and paper materials which have been so successfully applied in point-of-care tests, such as lateral-flow immunoassay strips and emerging microfluidic paper-based devices. We then turn our attention to the multifarious polymer designs that provide both the base materials for sensor designs, such as PDMS, and more advanced functionalised materials that are capable of both recognition and transduction, such as conducting and molecularly imprinted polymers. The subsequent content discusses wearable soft and flexible material-based affinity sensors, classified as flexible and skinmountable, textile materials-based and contact lens-based affinity sensors. In the final sections, we explore the possibilities for implantable/injectable soft and flexible affinity sensors, including hydrogels, microencapsulated sensors and optical fibers. This area is truly a work in 2 progress and we trust that this review will help pull together the many technological streams that are contributing to the field.

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“…The electrochemical signal that produced by probe hybridized with target DNA is used for qualitative and quantitative detection. Based on hybridization type, the electrochemical sensors were divided into two categories: heterogeneous hybridization (Cai et al, 2002 ;Wang 2006 ;Li et al, 2014 ;Song and Jin 2015 ;Ionescu et al, 2006) and homogeneous hybridization (Cui et al, 2014 ;Fan et al, 2011a ;Fan et al, 2011b ;Fan et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

An electrochemical DNA sensor without electrode pre-modification

Hong

Cheng

Wei

et al. 2017

Biosensors and Bioelectronics

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We present a non-modification electrochemical DNA sensing strategy, which used Potential-Assisted Au-S Deposition and a clamp-like DNA probe. The dual-hairpin probe DNA was tagged with a methylene blue (MB) at the 3' terminal and a thiol at the 5' terminal., Without being hybridized with target DNA, the loop of probe prevented the thiol from reaching the bare gold electrode surface with an applied potential., After hybridization with the target DNA, the probe' s loop-stem structure opened through two distinct and sequential events, which led to the formation of a triplex DNA structure. Then the thiol easily contacted with electrode and resulted in potential-assisted Au-S self-assembly. Electrochemical signals of MB were measured by differential pulse voltammetry (DPV) and used for target quantitative detection. This strategy offered a detection limit down to 2.3pM. and an inherently high specificity for detecting even single mismatch.

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Label-free Electrochemical Detection of the Human Adenovirus 40/41 Fiber Gene

Cited by 8 publications

References 34 publications

Recent Advances in Early Diagnosis of Viruses Associated with Gastroenteritis by Biosensors

Recent Advances in Early Diagnosis of Viruses Associated with Gastroenteritis by Biosensors

Soft and flexible material-based affinity sensors

An electrochemical DNA sensor without electrode pre-modification

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