Label-free electrochemical immunosensor for detection of oncomarker CA125 in serum

Castro, Ana Cristina Honorato de; Alves, Lívia Maria; Siquieroli, Ana Carolina Silva; Madurro, João M.; Brito‐Madurro, Ana G.

doi:10.1016/j.microc.2020.104746

Cited by 68 publications

(28 citation statements)

References 75 publications

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“…The redox pair interaction with the electrode surface changes based on the concentration of the antigen present on the solution. As a result, the electronic signal varies and allows for quantitative detection of the antigen [ 71 ]. The setup can be reversed, where the antigen is immobilized for specific antibody detection.…”

Section: Electrochemical Biosensorsmentioning

confidence: 99%

“…For early-stage diagnoses of cancers, tracking of the disease-specific biomarkers is essential. Biomarkers are characteristics biomolecules overexpressed in the beginning of carcinogenesis, either by the body immune system in response to the disease or by the tumor cell itself [ 11 , 71 , 97 , 100 ]. Biomarkers could be utilized to assess the responses from the body towards a specific treatment process for controlling disease [ 191 ].…”

Section: Mnp-based Ecbs For Biomolecule Detectionmentioning

confidence: 99%

“…A glucose sensor was developed that could be used with a smartphone for blood glucose level detection [ 298 ]. Castro et al reported the fabrication of label-free immunosensor strips for the sensitive detection of CA125 cancer biomarkers from human serum sample [ 71 ]. An ECB device has been reported for the rapid detection of the COVID-19 virus, that could be used as an alternative to the RT-qPCR-based 2019-nCoV test [ 296 ].…”

Section: Advances In Poct Devices: Prospects and Challenges In Thementioning

confidence: 99%

“…The figure shows the circuit setup that was used for connecting the ECB with the smartphone and detecting glucose through the CV technique [ 298 ]. ( b ) fabrication of a label-free immunosensor on a screen-printed carbon electrode (SPCE) strip for the detection of CA125 biomarkers from serum samples through the DPV technique [ 71 ]. ( c ) mechanism proposed for a clinically tested ECB for the simultaneous detection of multiplex pathogens for the diagnosis of urinary tract infection; (i) lysis of different bacteria through identification of 16S rRNA; (ii) hybridization with detector probes; (iii) combining with the capture probe immobilized on the electrode surface; (iv) binding of anti-fluorescein HRP tag to form sandwich system; and (v) generation of i-t current signal for a fixed potential that corresponded to the concentration of different bacteria present in the system [ 300 ].…”

Section: Figurementioning

confidence: 99%

“…( c ) mechanism proposed for a clinically tested ECB for the simultaneous detection of multiplex pathogens for the diagnosis of urinary tract infection; (i) lysis of different bacteria through identification of 16S rRNA; (ii) hybridization with detector probes; (iii) combining with the capture probe immobilized on the electrode surface; (iv) binding of anti-fluorescein HRP tag to form sandwich system; and (v) generation of i-t current signal for a fixed potential that corresponded to the concentration of different bacteria present in the system [ 300 ]. Reprinted with permission from [ 71 , 298 ], Copyright © 2020 and 2020 published by Elsevier B.V. [ 300 ], Copyright © 2020 and 2020 published by the American Urological Association. DAC: Digital-to-analog converter.…”

Section: Figurementioning

confidence: 99%

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Metal Nanoparticles for Electrochemical Sensing: Progress and Challenges in the Clinical Transition of Point-of-Care Testing

et al. 2020

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With the rise in public health awareness, research on point-of-care testing (POCT) has significantly advanced. Electrochemical biosensors (ECBs) are one of the most promising candidates for the future of POCT due to their quick and accurate response, ease of operation, and cost effectiveness. This review focuses on the use of metal nanoparticles (MNPs) for fabricating ECBs that has a potential to be used for POCT. The field has expanded remarkably from its initial enzymatic and immunosensor-based setups. This review provides a concise categorization of the ECBs to allow for a better understanding of the development process. The influence of structural aspects of MNPs in biocompatibility and effective sensor design has been explored. The advances in MNP-based ECBs for the detection of some of the most prominent cancer biomarkers (carcinoembryonic antigen (CEA), cancer antigen 125 (CA125), Herceptin-2 (HER2), etc.) and small biomolecules (glucose, dopamine, hydrogen peroxide, etc.) have been discussed in detail. Additionally, the novel coronavirus (2019-nCoV) ECBs have been briefly discussed. Beyond that, the limitations and challenges that ECBs face in clinical applications are examined and possible pathways for overcoming these limitations are discussed.

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Section: Electrochemical Biosensorsmentioning

confidence: 99%

Section: Mnp-based Ecbs For Biomolecule Detectionmentioning

confidence: 99%

Section: Advances In Poct Devices: Prospects and Challenges In Thementioning

confidence: 99%

Section: Figurementioning

confidence: 99%

Section: Figurementioning

confidence: 99%

See 3 more Smart Citations

Metal Nanoparticles for Electrochemical Sensing: Progress and Challenges in the Clinical Transition of Point-of-Care Testing

et al. 2020

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Oligonucleotic Probes and Immunosensors Based on Nanoporous Anodic Alumina for Screening of Diseases

Xifré-Pérez

Ferré‐Borrull

Marsal

2022

Adv Materials Technologies

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This paper is an overview of the recent advances in nanoporous anodic alumina sensing platforms based on the interaction of oligonucleotides and antibodies with bioelements (e.g., virus, bacteria, or biomarkers) that can cause or identify diseases. Nanoporous alumina is the common platform material for all these sensing devices that have an enormous potential for the next‐generation bioanalytical systems. Nanoporous alumina is a cost‐effective material with morphological flexibility that provides high sensitivity to the sensing systems due to its large surface‐to‐volume ratio. Immunosensors transduce the signal produced by the specific antibody–antigen interaction. They are widely used for clinical analysis due to the high selectivity and sensitivity of their immunoreaction, transduced by different methods (electrochemical, optical, piezoelectric, etc). Another important, outstanding and more recently type of biosensors are based on the oligonucleotic functionalization of nanoporous alumina. Oligonucleotides can bind to a wide variety of elements (small organic molecules, proteins, or cells) with high selectivity, specificity, and affinity, even higher to those of antibodies. The basic concepts and mechanisms of their sensing methods for health care biosensing are introduced. Their transduction methods and great potential applications will be evaluated and classified in detail, together with a discussion of possible future trends.

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Electro‐immunosensor for ultra‐sensitive determination of cardiac troponin I based on reduced graphene oxide and polytyramine

Brussasco

Guedes

Moço

et al. 2022

J of Molecular Recognition

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This work reports the construction of a novel nanostructured immunosensor for detection of the troponin I biomarker (cTnI). Anti-troponin I antibody was anchored on the modified graphite electrode with reduced graphene oxide and polytyramine for detection of troponin I in serum samples. The performance of the electro-immunosensor was evaluated by differential pulse voltammetry. The immunosensor presented a wide work range, from 4 ng mL À1 to 4 pg mL À1 , whose detection limit (4 pg mL À1 ) is significantly lower than the basal level in human serum, and maintained 100% of response after 30 days of storage. Moreover, the immunosensor showed good selectivity for detection of cTnI in real sample containing interfering substances and specificity of response to cTnI in the serum of healthy and sick patients, and demonstrated the possibility of reuse for two consecutive analyses, in addition to using a simplified and inexpensive platform when compared to other devices, demonstrating them excellent potential for application in diagnosis in the early stages of acute myocardial infarction.

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Label-free electrochemical immunosensor for detection of oncomarker CA125 in serum

Cited by 68 publications

References 75 publications

Metal Nanoparticles for Electrochemical Sensing: Progress and Challenges in the Clinical Transition of Point-of-Care Testing

Metal Nanoparticles for Electrochemical Sensing: Progress and Challenges in the Clinical Transition of Point-of-Care Testing

Oligonucleotic Probes and Immunosensors Based on Nanoporous Anodic Alumina for Screening of Diseases

Electro‐immunosensor for ultra‐sensitive determination of cardiac troponin I based on reduced graphene oxide and polytyramine

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