Label-free fluorescence turn-on detection of microRNA based on duplex-specific nuclease and a perylene probe

Human Serum Albumin-Occupying-Based Fluorescence Turn-On Analysis of Antiepileptic Drug Tiagabine Hydrochloride

“…As depicted in Scheme , a water-soluble organic molecule, perylene tetracarboxylic acid imide derivatives (PTAI), was exploited as the fluorescent indicator. PTAI have been used in biochemical sensing based on the discovery of DNA-templated self-aggregation. − Interestingly, in this work, we found that PTAI can specifically bind to HSA to greatly reduce its fluorescence quanta yield via embedding in the pocket of HSA’s binding region site II. This new observation called s binding-induced quenching (BIQ) would offer the candidate for target-activated fluorescent sensing.…”

Cascaded strand displacement for non-enzymatic target recycling amplification and label-free electronic detection of microRNA from tumor cells

“…Nevertheless, it still suffers the drawback of low sensitivity. To circumvent this limitation, a number of signal amplification approaches based on rolling circle amplification [15], nanoparticle-based approaches [16] and nuclease-assisted target recycling [13,17] have been reported to achieve high sensitivity for miRNA detection. Among these amplification approaches, the nuclease-assisted target recycling strategies have attracted considerable attention because of their significant singal amplificaiton capabilities.…”

A duplex-specific nuclease (DSN) and catalytic hairpin assembly (CHA)-mediated dual amplification method for miR-146b detection

“…8 MiRNAs are differentially expressed during the development of various cancers. 7,9,10 Therefore, miRNAs have gained much attention as biomarkers and are expected to be suitable for early clinical disease diagnosis and effective cancer therapeutics. 9,11,12 Moreover, specifically detecting mRNAs is challenging due to their intrinsically disadvantageous features, including short sequences, susceptibility to degradation, low abundance, and high sequence homology among family members.…”