Label-free Imaging of Myocardial Remodeling in Atrial Fibrillation Using Nonlinear Optical Microscopy: A Feasibility Study.

Büttner, Petra; Galli, Roberta; Husser, Daniela; Bollmann, Andreas

doi:10.4022/jafib.1644

Cited by 7 publications

(4 citation statements)

References 33 publications

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“…Cryo-embedded heart sections (8 µm) were placed on glass slides, kept moist by a drop of phosphate buffered saline, and natively used for NLOM which was performed using laser excitation provided by two picosecond near-infrared Erbium fiber sources. Technical details were previously described [ 41 ]. Three signals were simultaneously acquired and combined in one RGB image: coherent anti-Stokes Raman scattering (CARS) in the red channel, two-photon excited fluorescence (TPEF) in the green channel and second harmonic generation (SHG) in the blue channel.…”

Section: Methodsmentioning

confidence: 99%

Sacubitril/Valsartan Improves Diastolic Function But Not Skeletal Muscle Function in a Rat Model of HFpEF

Schauer

Adams

Augstein

et al. 2021

IJMS

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The angiotensin receptor/neprilysin inhibitor Sacubitril/Valsartan (Sac/Val) has been shown to be beneficial in patients suffering from heart failure with reduced ejection fraction (HFrEF). However, the impact of Sac/Val in patients presenting with heart failure with preserved ejection fraction (HFpEF) is not yet clearly resolved. The present study aimed to reveal the influence of the drug on the functionality of the myocardium, the skeletal muscle, and the vasculature in a rat model of HFpEF. Female obese ZSF-1 rats received Sac/Val as a daily oral gavage for 12 weeks. Left ventricle (LV) function was assessed every four weeks using echocardiography. Prior to organ removal, invasive hemodynamic measurements were performed in both ventricles. Vascular function of the carotid artery and skeletal muscle function were monitored. Sac/Val treatment reduced E/é ratios, left ventricular end diastolic pressure (LVEDP) and myocardial stiffness as well as myocardial fibrosis and heart weight compared to the obese control group. Sac/Val slightly improved endothelial function in the carotid artery but had no impact on skeletal muscle function. Our results demonstrate striking effects of Sac/Val on the myocardial structure and function in a rat model of HFpEF. While vasodilation was slightly improved, functionality of the skeletal muscle remained unaffected.

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Section: Methodsmentioning

confidence: 99%

Sacubitril/Valsartan Improves Diastolic Function But Not Skeletal Muscle Function in a Rat Model of HFpEF

Schauer

Adams

Augstein

et al. 2021

IJMS

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“…Especially SRS delivers significant vibrational information with high sensitivity, which-in combination with CARS-could improve the performance of the machine learning algorithm making it more robust to outliers and improving the overall diagnostic procedure, not only for FD but also for other diagnostic applications. Nonlinear optical microscopy has already demonstrated its added value in the diagnosis of a brain tumor [47] and lung carcinoma [48], as well as the visualization of myocardial tissue remodeling [49]. Recently it was shown that the Raman-based characterization of tumor tissue provided an exact localization of cancer-infiltrated regions ex-vivo and in-vivo [50].…”

Section: Discussionmentioning

confidence: 99%

CARS Imaging Advances Early Diagnosis of Cardiac Manifestation of Fabry Disease

Tolstik

Ali

Guo

et al. 2022

IJMS

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Vibrational spectroscopy can detect characteristic biomolecular signatures and thus has the potential to support diagnostics. Fabry disease (FD) is a lipid disorder disease that leads to accumulations of globotriaosylceramide in different organs, including the heart, which is particularly critical for the patient’s prognosis. Effective treatment options are available if initiated at early disease stages, but many patients are late- or under-diagnosed. Since Coherent anti-Stokes Raman (CARS) imaging has a high sensitivity for lipid/protein shifts, we applied CARS as a diagnostic tool to assess cardiac FD manifestation in an FD mouse model. CARS measurements combined with multivariate data analysis, including image preprocessing followed by image clustering and data-driven modeling, allowed for differentiation between FD and control groups. Indeed, CARS identified shifts of lipid/protein content between the two groups in cardiac tissue visually and by subsequent automated bioinformatic discrimination with a mean sensitivity of 90–96%. Of note, this genotype differentiation was successful at a very early time point during disease development when only kidneys are visibly affected by globotriaosylceramide depositions. Altogether, the sensitivity of CARS combined with multivariate analysis allows reliable diagnostic support of early FD organ manifestation and may thus improve diagnosis, prognosis, and possibly therapeutic monitoring of FD.

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“…Fibrosis is characterized by pronounced myocardial remodeling, so many optical approaches are capable of detecting fibrosis. Beyond conventional histology, Raman scattering, confocal and two-photon microscopy have been applied for detection and quantification of atrial fibrosis in ex-vivo samples [108,[126][127][128]. Furthermore, OCT was applied to classify fibrosis and other types of tissue remodeling in human atrial samples [129].…”

Section: Optical Approachesmentioning

confidence: 99%

Catheter-based optical approaches for cardiovascular medicine: progress, challenges and new directions

et al. 2020

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Catheterization of the heart is crucial for many diagnostic and therapeutic procedures in cardiovascular medicine. In this review, we discussed developments of catheter-based optical tools and approaches for cardiovascular medicine. We provided a background in gross and microscopic anatomy of the normal and diseased heart. We overviewed optical properties of cardiac tissues, such as scattering, absorption and fluorescence, and related optical properties to tissues constituents. Furthermore, we introduced optical modalities for tissue characterization, in particular, spectroscopy, confocal, multi-photon and light sheet fluorescence microcopy, and optical coherence tomography. We then surveyed example applications in cardiovascular medicine and contrasted established clinical tools and approaches with catheter-based optical approaches and tools. First, we explored assessment of heart transplant rejection and reviewed alternative catheterized optical approaches. Rejection is commonly assessed using endomyocardial biopsy, i.e. the excision and histological assessment of tissue samples. A further application is atrial fibrosis mapping. Atrial fibrosis is an important predictor for prognosis of atrial fibrillation patients, yet clinical tools for fibrosis mapping in patients are lacking. We surveyed clinical tools for assessing catheter ablation of the heart, which is an indispensable therapy for arrhythmia. Last, we discussed methods and protocols for guiding coronary angioplasty and stent placement. For all applications, we explored the current and potential role of catheterized optical tools. We concluded with a discussion of technical challenges and open questions related to clinical translation of the catheter-based optical approaches. Our review stressed the potential of catheterized optical tools to improve diagnosis and treatment of patients with heart disease.

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Label-free Imaging of Myocardial Remodeling in Atrial Fibrillation Using Nonlinear Optical Microscopy: A Feasibility Study.

Cited by 7 publications

References 33 publications

Sacubitril/Valsartan Improves Diastolic Function But Not Skeletal Muscle Function in a Rat Model of HFpEF

Sacubitril/Valsartan Improves Diastolic Function But Not Skeletal Muscle Function in a Rat Model of HFpEF

CARS Imaging Advances Early Diagnosis of Cardiac Manifestation of Fabry Disease

Catheter-based optical approaches for cardiovascular medicine: progress, challenges and new directions

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