Label-free platform on pH-responsive chitosan: Adhesive heterogeneity for cancer stem-like cell isolation from A549 cells via integrin β4

The human mesenchymal stem cells (hMSCs) therapy offering an encouraging the new methods to establish the conveying on the chitosan (C)/dextran (D)/β-glycerophosphate (β-GP) loaded with hMSCs to enhance the acute myocardial infarctions. The synthesized hMSCs-CD@β-GP system displayed the ratio of determination modules, size of the pore, absorbency, and the swellings ratio in the assortment of the 65 ka, 149 ± 39.8 µm, 92.2%, 42 ± 1.38, and 29 ± 1.9, respectively. The fabricated hMSCs-CD@β-GP was highly stable and physicochemical investigated and confirmed the suitability of the materials for cardiac regeneration applications. The in vitro examinations of the injectable hydrogels with hMSCs-CD@β-GP have recognized that the improved survival rate of the cells, increased the pro-inflammatory expressions factors, pro-angiogenic factors analysis confirmed the promising results of the ejection of fractions, fibrosis area, vessel density with decreased infractions size, with suggesting that the remarkable improvement of the heart regenerative function after myocardial infarctions. The new synergistic approach of the injectable hydrogels with hMSCs could able appropriate for the effective treatment of cardiac therapies after acute myocardial infarctions.

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“…Expanded cells were allowed for immunomagnetic separation with microbeads to obtain c-kit CSCs. 47 - 49 …”

Section: Methodsmentioning

confidence: 99%

Synergistic Fabrication of Dose–Response Chitosan/Dextran/β-Glycerophosphate Injectable Hydrogel as Cell Delivery Carrier for Cardiac Healing After Acute Myocardial Infarction

Chen

Liu

Hua³

et al. 2020

Dose-Response

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“…It is regulated at the transcriptional, posttranscriptional, and epigenetic levels, and posttranslational modifications play an important role in mucin binding and the clearance of microbes and pollutants [15]. It is overexpressed in lung cancer cells, interacts with integrin β4 to initiate FAK phosphorylation, and mediates the invasion and migration of lung cancer cells through chitosan separation of tumor stem-like cells [5,16]. TP53 mutation and integrin β4 overexpression co-occur in many aggressive malignancies, and lung squamous cell carcinoma has a higher TP53 mutation frequency; the overexpression of integrin β4 can also lead to venous infiltration and a reduction in overall survival in patients with non-small cell lung cancer [17].…”

Section: Lung Cancermentioning

confidence: 99%

“…In contrast to this function, many studies have shown that integrin α6β4 signals in various cancers to promote tumor invasion and metastasis [3]. Integrin β4 is closely related to lung cancer [5], breast cancer [6], prostate cancer [7], colon cancer [8], and other cancers. Differential expression of integrin β4 is found in these cancer tissues, and it is compatible with epidermal growth factor receptors in liposome interactions to promote cell growth and reproduction [6].…”

Section: Introductionmentioning

confidence: 99%

Integrin β4 as a Potential Diagnostic and Therapeutic Tumor Marker

Yang

Peng

et al. 2021

Biomolecules

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Integrin β4 (ITGβ4) is a class of transmembrane adhesion molecules composed of hemidesmosomes (HDs). Its unique long intracellular domain provides intricate signal transduction functions. These signal transduction effects are especially prominent in tumors. Many recent studies have shown that integrin β4 is differentially expressed in various tumors, and it plays a vital role in tumor invasion, proliferation, epithelial–mesenchymal transition, and angiogenesis. Therefore, we categorize the research related to integrin β4, starting from its structure and function in tumor tissues, and provide a basic description. Based on its structure and function, we believe that integrin β4 can be used as a tumor marker. In clinical practice, it is described as a diagnostic marker for the targeted treatment of cancer and will be helpful in the clinical diagnosis and treatment of tumors.

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“…CS is a naturally derived cationic polysaccharide that is composed of randomly distributed D-glucosamine (deacetylated units) and N -acetyl-D-glucosamine (acetylated units) linked by β-(1,4) glycosidic bonds with a unique isoelectric point that appears at the restricted physiological pH (pH 7.4) [ 15 , 16 ]. In our previous works, we demonstrated that CS could control cell attachment/detachment in response to subtle pH variations to recover cultured cells without additional enzymatic treatments and extensive washing steps [ [17] , [18] , [19] ]. Moreover, numerous studies have documented the enhanced pluripotency of MSCs by culturing them on CS or CS-based substrates [ [20] , [21] , [22] ].…”

Section: Introductionmentioning

confidence: 99%

pH-driven continuous stem cell production with enhanced regenerative capacity from polyamide/chitosan surfaces

Yen

Cheng

Hsieh

et al. 2023

Materials Today Bio

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Label-free platform on pH-responsive chitosan: Adhesive heterogeneity for cancer stem-like cell isolation from A549 cells via integrin β4

Cited by 6 publications

References 50 publications

Synergistic Fabrication of Dose–Response Chitosan/Dextran/β-Glycerophosphate Injectable Hydrogel as Cell Delivery Carrier for Cardiac Healing After Acute Myocardial Infarction

Synergistic Fabrication of Dose–Response Chitosan/Dextran/β-Glycerophosphate Injectable Hydrogel as Cell Delivery Carrier for Cardiac Healing After Acute Myocardial Infarction

Integrin β4 as a Potential Diagnostic and Therapeutic Tumor Marker

pH-driven continuous stem cell production with enhanced regenerative capacity from polyamide/chitosan surfaces

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