Labeling and biological evaluation of 99m Tc-azithromycin for infective inflammation diagnosis

Sanad, M. H.

doi:10.1134/s1066362213050159

Cited by 53 publications

(28 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Inflammation was induced with direct injection of turpentine oil and heat killed bacteria. The quantitative evaluation, expressed as the percentage of injected dose per organ, showed an excretion through kidneys and urine, and high accumulation in infectious muscle than controls, confirmed by the T /NT ratio: the maximum peak was 6.20 ± 0.12 at 2 h post-injection, but at all time intervals values were significantly higher than sterile inflamed muscles [115]. …”

Section: Inhibitors Of Protein Synthesismentioning

confidence: 99%

“…Hina [113]Direct labelling—SnCl 2 ·2H 2 O7.8 × 10 5 95 % at 12 h98 % at 24 hRatLiver (++), kidneys (+) 99m Tc-azithromycinM. H. Sanad [115]Direct labelling—SnCl 2 ·2H 2 O1.44 × 10 5 97.5 % at 2 h85.5 % at 24 hMouseLiver (+), kidneys (++) 99m Tc-clindamycinS. Hina [118]Direct labelling—SnCl 2 ·2H 2 O1.56 × 10 6 95 % at 5 h92 % at 24 hRatLiver (+), kidneys (++)Others6-[ 18 F]-fluoromaltoseG.…”

Section: Summary Of Systematic Analysis Of the Literaturementioning

confidence: 99%

See 1 more Smart Citation

Imaging bacteria with radiolabelled quinolones, cephalosporins and siderophores for imaging infection: a systematic review

Auletta

Galli

Lauri

et al. 2016

Clin Transl Imaging

View full text Add to dashboard Cite

Bacterial infections are still one of the main causes of patient morbidity and mortality worldwide. Nowadays, many imaging techniques, like computed tomography or magnetic resonance imaging, are used to identify inflammatory processes, but, although they recognize anatomical modifications, they cannot easily distinguish bacterial infective foci from non bacterial infections. In nuclear medicine, many efforts have been made to develop specific radiopharmaceuticals to discriminate infection from sterile inflammation. Several compounds (antimicrobial peptides, leukocytes, cytokines, antibiotics…) have been radiolabelled and tested in vitro and in vivo, but none proved to be highly specific for bacteria. Indeed factors, including the number and strain of bacteria, the infection site, and the host condition may affect the specificity of tested radiopharmaceuticals. Ciprofloxacin has been proposed and intensively studied because of its easy radiolabelling method, broad spectrum, and low cost, but at the same time it presents some problems such as low stability or the risk of antibiotic resistance. Therefore, in the present review studies with ciprofloxacin and other radiolabelled antibiotics as possible substitutes of ciprofloxacin are reported. Among them we can distinguish different classes, such as cephalosporins, fluoroquinolones, inhibitors of nucleic acid synthesis, inhibitors of bacterial cell wall synthesis and inhibitors of protein synthesis; then also others, like siderophores or maltodextrin-based probes, have been discussed as bacterial infection imaging agents. A systematic analysis was performed to report the main characteristics and differences of each antibiotic to provide an overview about the state of the art of imaging infection with radiolabelled antibiotics.

show abstract

Section: Inhibitors Of Protein Synthesismentioning

confidence: 99%

Section: Summary Of Systematic Analysis Of the Literaturementioning

confidence: 99%

Imaging bacteria with radiolabelled quinolones, cephalosporins and siderophores for imaging infection: a systematic review

Auletta

Galli

Lauri

et al. 2016

Clin Transl Imaging

View full text Add to dashboard Cite

show abstract

“…Iodine 123 is the isotope of choice for medical imaging with a half‐life to 13.22 hours and decays by emission of gamma radiation with an energy of 159 keV. However, its short half‐life and expense necessitate that iodine 125 be used to develop the labeling procedure and evaluate the biodistribution in mice . Two different groups of mice, one bearing stomach ulcers and the other not, were injected with [ 125 I]iodonizatidine and the results were compared.…”

Section: Introductionmentioning

confidence: 99%

Radioiodination and biological evaluation of nizatidine as a new highly selective radiotracer for peptic ulcer disorder detection

Sanad

Saleh

Marzook

2017

Labelled Comp Radiopharmac

View full text Add to dashboard Cite

Nizatidine has been labeled using [ I] with chloramine-T as oxidizing agent. Factors such as the amount of oxidizing agent, amount of substrate, pH, reaction temperature, and reaction time have been systematically studied to optimize the iodination. Biodistribution studies indicate the suitability of radioiodinated nizatidine as a novel tracer to image stomach ulcer. Radioiodinated nizatidine may be considered a highly selective radiotracer for peptic ulcer imaging.

show abstract

“…177 Lu emits both beta (β) and gamma (γ) particles; β‐particle emissions ( E max = 487, 383 and 175 keV) are used for remedy purpose of tumor and bone ache palliation, etc. and γ‐radiation (113 and 208 keV) for imaging …”

Section: Introductionmentioning

confidence: 99%

“…and γ-radiation (113 and 208 keV) for imaging. [10][11][12][13][14][15] Kanamycin is an aminoglycoside antibiotic isolated from bacteria Streptomyces kanamyceticus. It is mostly used to treat serious bacterial infections in several body parts.…”

Section: Introductionmentioning

confidence: 99%

Radiolabeling, quality control, and biological characterization of ¹⁷⁷Lu‐labeled kanamycin

et al. 2017

View full text Add to dashboard Cite

Kanamycin is an antibiotic, isolated from Streptomyces kanamyceticus, which is used to treat serious bacterial infections. The fact that the present radioligand Tc-kanamycin used for diagnosis is short-lived, raised a need to label and study kanamycin with one of the most important beta (β) radiation emitting isotope Lu. Labeling yield of Lu-kanamycin was confirmed by different chromatography techniques such as paper chromatography, TLC, HPLC. Several experiments were performed to optimize labeling with changing reaction conditions such as pH, temperature, amount of ligand, and reaction time. In vitro stability analysis was performed incubation with human serum. Electrophoresis analysis was also conducted to determine the charge on Lu-kanamycin. The biodistribution and scintigraphy were performed in normal mice and rabbit, respectively, at different time intervals of postinjection. Lu-kanamycin was prepared with very high yield (~100%), with excellent stability in vivo and in vitro (>99% 6 hr postprep.), at pH 7. Maximum labeling was achieved at less reaction time (15 min), with maximum conjugation of the ligand (12.5 mg) with Lu. Electrophoresis analysis showed net neutral charge. The radioligand showed rapid clearance from body in biodistribution and scintigraphy studies. The preparation Lu-kanamycin could be used as a radio-pharmaceutical for infection imaging purpose, especially when transporting the radioligand to long-range distances.

show abstract

Labeling and biological evaluation of 99m Tc-azithromycin for infective inflammation diagnosis

Cited by 53 publications

References 20 publications

Imaging bacteria with radiolabelled quinolones, cephalosporins and siderophores for imaging infection: a systematic review

Imaging bacteria with radiolabelled quinolones, cephalosporins and siderophores for imaging infection: a systematic review

Radioiodination and biological evaluation of nizatidine as a new highly selective radiotracer for peptic ulcer disorder detection

Radiolabeling, quality control, and biological characterization of ¹⁷⁷Lu‐labeled kanamycin

Contact Info

Product

Resources

About

Labeling and biological evaluation of 99m Tc-azithromycin for infective inflammation diagnosis

Cited by 53 publications

References 20 publications

Imaging bacteria with radiolabelled quinolones, cephalosporins and siderophores for imaging infection: a systematic review

Imaging bacteria with radiolabelled quinolones, cephalosporins and siderophores for imaging infection: a systematic review

Radioiodination and biological evaluation of nizatidine as a new highly selective radiotracer for peptic ulcer disorder detection

Radiolabeling, quality control, and biological characterization of 177Lu‐labeled kanamycin

Contact Info

Product

Resources

About

Radiolabeling, quality control, and biological characterization of ¹⁷⁷Lu‐labeled kanamycin