Labeling of atenolol with radioactive iodine-125 using N-bromosuccinimide and hydrogen peroxide as oxidizing agents

Studies on the Labeling of Adrenocorticotropic Hormone (ACTH) by 125I Using Different Oxidizing Agents for Radioimmunoassay Purposes

“…In a water bath, the reaction mixture was kept 10 minutes. Then reaction mixture was to plane eppendorf tube [8][9][10][11][12][13]. The 125 I-ACTH tracer was purified using PD-10 sephadex column.…”

“…The preparation of 125 I-ACTH tracer was performed using enzymatic lactoperoxidase and urea hydrogen peroxide as oxidation technique that mentioned by El-Tawoosy et al 2011 [12]. To an eppendorf tube containing 2 µg of ACTH in 10 µl assay phosphate buffer, 10 µl of 0.5M assay buffer (pH 7.4) and 4 µl of Na-125 I (400 µCi, 14.8 MBq) were added..…”

Radioiodination and biological evaluation of nizatidine as a new highly selective radiotracer for peptic ulcer disorder detection

“…At reduced pressure, the solvent was removed. The solid that formed was collected, thoroughly washed with diethyl ether, crystallized from tetrahydrofuran, and purified by column chromatography (silica hexane:ethyl acetate 2:1 v/v), giving yellow crystals (0.482 g, 35%) …”

Radiochemistry

“…144 Other standard procedures for labelling molecules with radio-iodine are the chloramine-T method, used in the preparation of [ 125 I]iodotroxacitabine, 145 [ 125 I]iodoquercetin 146 and [ 131 I]iodochlorotoxin, 147 the iodogen procedure which was used to label 8-hydroxyquinoline glucuronide 148 with iodine-125 and -131, the lactoperoxidase method facilitated the labelling of [ 125 I]iodoerythropoietin 149 and the use of hydrogen peroxide as an oxidising agent to make [ 131 I] iodobetulinic acid 150 and [ 131 I] iodoatenolol. 151 It seems possible to 'label' nanoparticles and nanorods with radio-iodine. [ 131 I]iodo-L-tyrosine has been grafted 152 onto an iron oxide/silica composite and iodine-125 coated gold 153,154 and silver 155 nanorods have been made for radiotherapy.…”