Labeling PLA-PEG nanocarriers with IR780: physical entrapment versus covalent attachment to polylactide

Machado, Marina Guimarães Carvalho; Pound-Lana, Gwenaelle; Oliveira, Maria Alice de; Lanna, Elisa Gomes; Fialho, Márcia Célia Pacheco; Brito, Ana Carolina Ferreira de; Barboza, Ana Paula Moreira; Aguiar-Soares, Rodrigo Dian de Oliveira; Mosqueira, Vanessa Carla Furtado

doi:10.1007/s13346-020-00812-6

Cited by 22 publications

(11 citation statements)

References 47 publications

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“…Values of the shape factor < 0.7 suggest particles with a dense core surrounded by a lighter shell [90]. Sometimes determining the shape of a particle only on the basis of the shape factor may lead to results of difficult physical interpretation [91,92]. Potential sources of error on the r g can be the coelution of materials of similar hydrodynamic size, but different shape or mass distribution, as a nanoparticle and a protein [93].…”

Section: Particle Shapementioning

confidence: 99%

“…The application of AF4 to the study of biocorona is twofold: on the one hand, differential FFF can be applied to study corona formation by monitoring differences in size distribution before and after the incubation in biological media [91,176]. On the other hand, it can serve as a fractionation technique that precedes the ex situ proteomic analysis, carried out by other means (electrophoresis, mass spectrometry).…”

Section: Study the Interactions With Biomolecules And Protein Coronamentioning

confidence: 99%

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Asymmetric flow field-flow fractionation as a multifunctional technique for the characterization of polymeric nanocarriers

Quattrini

Berrecoso

Crecente‐Campo

et al. 2021

Drug Deliv. and Transl. Res.

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The importance of polymeric nanocarriers in the field of drug delivery is ever-increasing, and the accurate characterization of their properties is paramount to understand and predict their behavior. Asymmetric flow field-flow fractionation (AF4) is a fractionation technique that has gained considerable attention for its gentle separation conditions, broad working range, and versatility. AF4 can be hyphenated to a plurality of concentration and size detectors, thus permitting the analysis of the multifunctionality of nanomaterials. Despite this potential, the practical information that can be retrieved by AF4 and its possible applications are still rather unfamiliar to the pharmaceutical scientist. This review was conceived as a primer that clearly states the “do’s and don’ts” about AF4 applied to the characterization of polymeric nanocarriers. Aside from size characterization, AF4 can be beneficial during formulation optimization, for drug loading and drug release determination and for the study of interactions among biomaterials. It will focus mainly on the advances made in the last 5 years, as well as indicating the problematics on the consensus, which have not been reached yet. Methodological recommendations for several case studies will be also included. Graphical abstract

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Section: Particle Shapementioning

confidence: 99%

Section: Study the Interactions With Biomolecules And Protein Coronamentioning

confidence: 99%

Asymmetric flow field-flow fractionation as a multifunctional technique for the characterization of polymeric nanocarriers

Quattrini

Berrecoso

Crecente‐Campo

et al. 2021

Drug Deliv. and Transl. Res.

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“…The suspension was shaken for 2 h (200 rpm, 25 °C) and then centrifuged at 10000 rpm for 10 min. Filter the supernatant with 0.22 μm water membrane and the concentration of IR780 in supernatants obtained again was determined by HPLC 50 . Carried on Agilent® XDB C18 column (4.6 mm × 250 mm, 5 μm) at 30 °C, the chromatographic separation was accomplished by mobile phase consisting of acetonitrile and water (65:35) with 0.1% acetic acid in an isocratic elution.…”

Section: Methodsmentioning

confidence: 99%

A hypoxia-responsive supramolecular formulation for imaging-guided photothermal therapy

Zhang¹,

Hou²,

Kong³

et al. 2022

Theranostics

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Photothermal agents (PTAs) based on organic small-molecule dyes emerge as promising theranostic strategy in imaging and photothermal therapy (PTT). However, hydrophobicity, photodegradation, and low signal-to-noise ratio impede their transformation from bench to bedside. In this study, a novel supramolecular PTT formulation by a stimuli-responsive macrocyclic host is prepared to overcome these obstacles of organic small-molecule PTAs. Methods: Sulfonated azocalix[4]arene (SAC4A) was synthesized as a hypoxia-responsive macrocyclic host. Taking IR780 as an example, the supramolecular nanoformulation IR780@SAC4A was constructed by grinding method, and its solubility, photostability, and photothermal conversion were evaluated. The hypoxia tumor-selective imaging and supramolecular PTT of IR780@SAC4A were further evaluated in vitro and in vivo . Results: IR780@SAC4A is capable of enhancing the solubility, photostability, and photothermal conversion of IR780 significantly, which achieve this supramolecular formulation with good imaging-guided PTT efficacy in vitro and in vivo . Conclusions: This study demonstrates that the supramolecular PTT strategy is a promising cancer theranostic method. Moreover, this supramolecular approach is applicative to construct kinds of supramolecular PTAs, opening a general avenue for extending smart PTT formulations.

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“… 2 , 3 Despite the huge interest in water-based coatings, only a few studies account for the preparation of PLA dispersions in water. 4 − 6 Furthermore, in most of them, halogenated solvents are employed to solubilize the polymer during the preparation of the aqueous dispersion, preventing further use in many applications, in particular the food contact ones. 6 An effective and environmentally friendly method to prepare PLA aqueous dispersions suitable for many applications, including thin coatings for food packaging, has been recently developed by our group.…”

Section: Introductionmentioning

confidence: 99%

“…4 − 6 Furthermore, in most of them, halogenated solvents are employed to solubilize the polymer during the preparation of the aqueous dispersion, preventing further use in many applications, in particular the food contact ones. 6 An effective and environmentally friendly method to prepare PLA aqueous dispersions suitable for many applications, including thin coatings for food packaging, has been recently developed by our group. 5 The preparation strategy provides very stable dispersions that can be stored for several months and later used to prepare films and coatings.…”

Section: Introductionmentioning

confidence: 99%

Rheological Response of Polylactic Acid Dispersions in Water with Xanthan Gum

et al. 2022

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In this work, the rheological behavior of stable poly(lactic acid) (PLA) dispersions in water, intended for coating applications, was investigated. The newly prepared dispersion consists of PLA particles with an average diameter of 222 ± 2 nm based on dynamic light scattering (DLS) and scanning electron microscopy (SEM) analyses, at concentrations varying in the 5–22 wt % range. Xanthan gum (XG), a bacterial polysaccharide, was used as a thickening agent to modulate the viscosity of the formulations. The rheological properties of the PLA dispersions with different XG and PLA contents were studied in steady shear, amplitude sweep, and frequency sweep experiments. Under steady shear conditions, the viscosity of all the formulations showed a shear-thinning behavior similar to XG solutions in the whole investigated 1–1000 s –1 range, with values dependent on both PLA particles and XG concentrations. Amplitude and frequency sweep data revealed a weak-gel behavior except in the case of the most diluted sample, with moduli dependent on both PLA and XG contents. A unified scaling parameter was identified in the volume fraction (ϕ) of the PLA particles, calculated by considering the dependence of the continuous phase density on the XG concentration. Accordingly, a master curve at different volume fractions was built using the time–concentration–superposition approach. The master curve describes the rheological response of the system over a wider frequency window than the experimentally accessible one and reveals the presence of a superimposed β relaxation process in the high-frequency region.

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Labeling PLA-PEG nanocarriers with IR780: physical entrapment versus covalent attachment to polylactide

Cited by 22 publications

References 47 publications

Asymmetric flow field-flow fractionation as a multifunctional technique for the characterization of polymeric nanocarriers

Asymmetric flow field-flow fractionation as a multifunctional technique for the characterization of polymeric nanocarriers

A hypoxia-responsive supramolecular formulation for imaging-guided photothermal therapy

Rheological Response of Polylactic Acid Dispersions in Water with Xanthan Gum

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