Laboratory Aids to Diagnosis and Therapy

The Anti-thrombotic Active Constituents from Centella asiatica

“…There have been many investigations on the bioactivity of this plant, but most of these publications are of studies of the well-known asiaticoside (6), the main saponin. [9][10][11][12][13][14][15][16][17][18] We were unable to find any papers referring to anti-thrombotic effects of C. asiatica. It is known that platelet-rich thrombi play a pivotal role in myocardial infarction and stroke, having formed at sites of damage or stenosis in arteries.…”

“…However, despite the many reports on the saponins (contain asiaticoside) isolated from C. asiatica, [9][10][11][12][13][14][15][16][17][18] no anti-thrombotic compounds or anti-thrombotic effects have been reported with regard to this plant.…”

“…[19][20][21] Thus, we studied the inhibitory effect of constituents isolated from C. asiatica on thrombosis, using this haemostatometry. By this we found a significantly active compound, 3,5-di-O-caffeoyl quinic acid (2), [22][23][24] together with chlorogenic acid (1), [22][23][24] 1,5-di-O-caffeoylquinic acid (3), 25) 3,4-di-O-caffeoylquinic acid (4), [22][23][24] 4,5-di-O-caffeoylquinic acid (5), [22][23][24] and also the flavonoids, kaempferol (7), quercetin (8), kaempferol 3-O-b-D-glucuronide (9), 26) quercetin-3-O-b-D-glucuronide (10), 26,27) and the saponin, asiaticoside (6). 10,18) In this paper, we report an active anti-thrombotic compound, and the isolation and characterization of ten constituents isolated from this plant.…”

Structural role of zinc in biodegradation of alkali-free bioactive glasses

“…[5][6][7][8][9][10][11][12] It is an essential trace mineral for DNA synthesis, cell division and protein synthesis during the proliferative phase of wound healing. 13 Zinc deciency has been associated with poor wound healing and decreased breaking strength of animal wounds 5 which can result from decreased protein and collagen synthesis during healing found in zinc decient animals. Agren 5 demonstrated the benecial aspect of topically applied zinc on leg ulcer healing and wound healing in animal models.…”

Laboratory Aids to Diagnosis and Therapy

“…The procedure has proved invaluable in the elucidation of certain physiological problems, and its utility in the differential diagnosis of thyroid disorders has been established. This reviewer does not, however, share the enthusiasm of his predecessor (23) for its general adoption as a routine clinical procedure. The use of radioactive isotopes, even in tracer doses, can not be safely en- trusted to inexpert hands without adequate facilities.…”