Laboratory and clinical evaluation of isolation media for Campylobacter jejuni

Gun-Munro, J; Rennie, Robert; Thornley, J. H.; Richardson, Harold; Hodge, D S; Lynch, John

doi:10.1128/jcm.25.12.2274-2277.1987

Cited by 44 publications

(18 citation statements)

References 12 publications

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“…Traditionally, mCCDA medium has been widely used for the recovery of campylobacters (Hutchinson and Bolton, 1984;Gun-Munro et al, 1987). Plating onto CAT agar (Aspinall et al, 1993) has since been shown to be superior to mCCDA agar and more sensitive for isolation of a wider variety of Campylobacter spp.…”

Section: Resultsmentioning

confidence: 99%

“…The modified charcoal cefoperazone deoxycholate agar (mCCDA) medium has been used traditionally for the recovery of campylobacters (Hutchinson and Bolton, 1984;Gun-Munro et al, 1987). Methods developed subsequently for Campylobacter isolation include a membrane filtration technique and direct plating on basal agar containing cefoperazone, amphotericin and teicoplanin (CAT) selective agents.…”

Section: Introductionmentioning

confidence: 99%

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A Comparison of Different Culture Methods for the Recovery ofCampylobacterSpecies from Pets

Acke

McGill

Golden

et al. 2009

Zoonoses and Public Health

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Five culture methods for the recovery of Campylobacter species (spp.) were evaluated on 361 rectal swabs collected from cats and dogs in Ireland. Speciation using PCR methods was performed on all isolates to assess the sensitivity of each culture method for isolation of Campylobacter spp., and to establish the prevalence of Campylobacter jejuni, C. coli, C. upsaliensis, C. lari and C. helveticus. Overall 163 of 361 (45.2%) samples were confirmed Campylobacter spp. positive. Direct plating onto modified charcoal cefoperazone deoxycholate agar (mCCDA) with cefoperazone, amphotericin and teicoplanin (CAT) selective supplement yielded a significantly higher prevalence of Campylobacter spp. (33.0%) than each of the other four methods (P < or = 0.05). This method was also the most sensitive method for isolation of C. upsaliensis compared with any of the other four methods used in the current study (P < or = 0.05). A direct plating method onto mCCDA agar with CCDA selective supplement and a filtration method onto blood agar after pre-enrichment in CAT supplemented broth yielded lower Campylobacter spp. prevalences of 19.7% and 17.5% respectively. A filtration method onto CAT agar and pre-enrichment in Preston broth before plating onto mCCDA agar were less sensitive for the isolation of Campylobacter spp. Speciation results of Campylobacter isolates revealed the majority of Campylobacter isolates were C. upsaliensis (50.0%) and C. jejuni (41.9%). A small number of isolates were C. coli (2.6%), C. lari (1.5%) and C. helveticus (1.1%). The overall detection of Campylobacter spp. in the 361 pets sampled was significantly increased by using a combination of isolation methods (P < or = 0.05), producing a more accurate determination of the prevalence of Campylobacter spp. in pets in Ireland and of the actual Campylobacter species. As the majority of Campylobacter spp. were recovered by direct plating onto mCCDA agar with CAT supplement, this method is the method of choice if only a single method is selected for isolation of the most common Campylobacter spp. detected in pets and humans.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A Comparison of Different Culture Methods for the Recovery ofCampylobacterSpecies from Pets

Acke

McGill

Golden

et al. 2009

Zoonoses and Public Health

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“…Preston agar was less effective than either mCCDA or Karmali with the growth of other competing bacteria making the identification of Campylobacter colonies difficult. Preston agar has a different composition and has been reported as being less selective and less effective for direct isolation of Campylobacter from human faecal samples than mCCDA and Karmali agars (Merino et al 1986;Gun-Munro et al 1987).…”

Section: Discussionmentioning

confidence: 99%

An evaluation of survival and detection of Campylobacter jejuni and C. coli in broiler caecal contents using culture-based methods

Rodgers¹,

Clifton-Hadley²,

Marín

et al. 2010

Journal of Applied Microbiology

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Aims: To evaluate the culture specifications of the 2008 EU baseline survey for Campylobacter spp. in broiler flocks at slaughter, by assessing the detection of thermophilic Campylobacter in chicken caecal contents by culture on selective agar with or without enrichment culture. Additionally, to assess the impact of sample storage time on Campylobacter detection. Methods and Results: Serial dilutions of pooled caeca samples in phosphate‐buffered saline or Campylobacter‐negative caecal contents were cultured micro‐aerobically at 41·5°C on mCCDA, Karmali and Preston agars before and after enrichment in Exeter broth. Direct culture on mCCDA showed a higher isolation rate than for Karmali or Preston agars, but a similar isolation rate to enrichment. Enumeration of samples showed the numbers of viable bacteria dropped slightly during storage. Conclusions: Direct culture on mCCDA was the most sensitive method for detection of Campylobacter, and samples with 104 CFU g−1 were still detectable after 6 days. Significance and Impact of the Study: Comparison of prevalence results from the 2008 EU baseline survey will need careful interpretation as the different media specified vary in their sensitivity to detect thermophilic Campylobacter. Delayed culture for up to 80 h after collection should have little impact on detection rate.

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“…Microbial analysis of samples was by a five-tube MPN method (Donnison 2002); briefly, primary enrichment was in a Exeter broth (Martin et al 1996) with resuscitation for 24 h at 37°C followed by transfer to 42°C for a further 24 h. Primary enrichment was followed by secondary selective plating on modified charcoal cefoperazone agar (mCCDA; Oxoid, Basingstoke, UK) with plates incubated in a microaerophilic atmosphere (CampyGen, Oxoid, UK) for 24 h at 42°C. mCCDA has been demonstrated to recover campylobacter effectively (Gun-Munro et al 1987;Corry et al 1995;Engberg et al 2000). Its selectivity is due to inhibition of nontarget micro-organisms by the antibiotics cefoperazone and amphotericin B, and promotion of campylobacter growth due to inclusion of quenching and detoxifying agents (Bolton et al 1984).…”

Section: Analysis Of Bacteriamentioning

confidence: 99%

Campylobacter jejuni inactivation in New Zealand soils

Ross

Donnison

2006

J Appl Microbiol

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Aim: The study was undertaken to determine the inactivation rate of Campylobacter jejuni in New Zealand soils. Methods and Results: Farm dairy effluent (FDE) inoculated at c. 105 ml−1 with C. jejuni was applied to intact soil cores at a rate of 2 l m−2. Four soils were used: Hamilton (granular); Taupo (pumice); Horotiu and Waihou (allophanic). After FDE application cores were incubated at 10°C for up to 32 days. For all four soils all the FDE remained within the cores and at least 99% of C. jejuni were retained in the top 5 cm. Campylobacter jejuni had declined to the limit of detection (two C. jejuni 100 g−1) by 25 days in Hamilton and Taupo soils and by 32 days in Waihou soil. In contrast, in Horotiu soil the decline was only three orders of magnitude after 32 days. Simulated heavy rainfall was applied 4 and 11 days after FDE application and only about 1% of the applied C. jejuni were recovered in leachates. Conclusions: This study demonstrated that at least 99% of applied C. jejuni were retained in the top 5 cm of four soils where they survived for at least 25 days at 10°C. Significance and Impact of the Study: Soil retention of C. jejuni is efficient at FDE application rates that prevent drainage losses. The low infectious dose of C. jejuni and its ability to survive up to 25 days have implications for stock management on dairy farms.

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Laboratory and clinical evaluation of isolation media for Campylobacter jejuni

Cited by 44 publications

References 12 publications

A Comparison of Different Culture Methods for the Recovery ofCampylobacterSpecies from Pets

A Comparison of Different Culture Methods for the Recovery ofCampylobacterSpecies from Pets

An evaluation of survival and detection of Campylobacter jejuni and C. coli in broiler caecal contents using culture-based methods

Campylobacter jejuni inactivation in New Zealand soils

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