An evaluation of survival and detection of Campylobacter jejuni and C. coli in broiler caecal contents using culture-based methods

Rodgers, John; Clifton-Hadley, F. A.; Marín, Clara; Vidal, Ana Maria Centola

doi:10.1111/j.1365-2672.2010.04748.x

Cited by 20 publications

(21 citation statements)

References 40 publications

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“…Six STs (one C. jejuni and five C. coli ) were recovered at the same low frequency from both direct and enriched culture, while many more were seen on one occasion only, irrespective of the culture method used. Despite concerns that Exeter broth is more selective for C. jejuni due to its Polymixin B content, the predominant growth of C. coli from the wild ducks suggests that isolation of this species was not prevented in this case (Humphrey, 1989; Rodgers et al ., 2010).…”

Section: Resultsmentioning

confidence: 99%

Campylobacter populations in wild and domesticated Mallard ducks (Anas platyrhynchos)

Colles

Ali

Sheppard

et al. 2011

Environ Microbiol Rep

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Identifying the Campylobacter genotypes that colonize farmed and wild ducks will help to assess the proportion of human disease that is potentially attributable to the consumption of duck meat and environmental exposure to duck faeces. Comparison of temporally and geographically matched farmed and wild ducks showed that they had different Campylobacter populations in terms of: (i) prevalence, (ii) Campylobacter species and (iii) diversity of genotypes. Furthermore, 92.4% of Campylobacter isolates from farmed ducks were sequence types (STs) commonly associated with human disease, in contrast to just one isolate from the wild ducks. Only one ST, ST-45, was shared between the two sources, accounting for 0.9% of wild duck isolates and 5% of farmed duck isolates. These results indicate that domestic ‘niche’ as well as host type may affect the distribution of Campylobacter, and that husbandry practises associated with intensive agriculture may be involved in generating a reservoir of human disease associated lineages.

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Section: Resultsmentioning

confidence: 99%

Campylobacter populations in wild and domesticated Mallard ducks (Anas platyrhynchos)

Colles

Ali

Sheppard

et al. 2011

Environ Microbiol Rep

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“…False negative by direct plating might indicate cases in which stressed or injured cells were below the quantification limit for the direct culture; however, parallel detection after 48 h in enrichment enabled their recovery (Gharst et al., 2006). On the contrary, campylobacters that have been detected by direct plating, but not by parallel enrichment, may have been out‐competed by other microflora in the broth culture (Musgrove et al., 2001) or may have been strains that were sensitive to the selective agents present in the enrichment broth (Rodgers et al., 2010).…”

Section: Discussionmentioning

confidence: 99%

“…in broiler flocks in the EU (Anonymous 2007). The processing and culture methods were as described by Rodgers et al. (2010).…”

Section: Methodsmentioning

confidence: 99%

Comparison of Different Sampling Strategies and Laboratory Methods for the Detection of C. jejuni and C. coli from Broiler Flocks at Primary Production

Vidal

Rodgers

Arnold

et al. 2012

Zoonoses and Public Health

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The objective of the study was to evaluate the performance of different combinations of sample type, transport medium and culture methods for the recovery of Campylobacter jejuni and C. coli from broiler flocks at primary production. Boot swabs moistened with one of four different transport media [maximum recovery diluent (n=120), Exeter broth (EX) (n=120), buffered peptone water (n=120) and modified semi-solid Cary-Blair (n=120)], caecal samples (n=40) and faecal samples (n=120) from 40 broiler flocks were compared and sensitivity estimates obtained using a Bayesian model. Samples were cultured onto mCCDA before and after enrichment in EX and incubated microaerobically at 41.5°C. Campylobacter suspect colonies were identified to the species level by multiplex PCR. Results from the Bayesian model indicated that boot swabs after enrichment had higher sensitivity (90-94%) than caecal contents before or after enrichment (84% and 89%, respectively) and faecal samples after enrichment (82%) for the detection of Campylobacter spp., although these differences were not statistically significant. Enrichment significantly increased the sensitivity of boot swab and caecal samples for detection of Campylobacter spp. and C. jejuni, respectively. However, the enrichment of caecal samples resulted in a significant decrease in the sensitivity of these samples for detection of C. coli. There was much greater variation in the sensitivity estimates of the methods for detecting C. coli than for C. jejuni, and the ranking of methods was different between the two species. Boot swabs gave the best sensitivity values for detection of C. jejuni, and enrichment culture of faecal samples was the most sensitive method for detection of C. coli.

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“…This is possibly due to the intrinsic higher limit of detection of the PCR (>10 5 c.f.u./g) [12] compared to culture (>10 2 c.f.u./ g) [19] and therefore caecal samples with lower concentrations of bacteria will not be detected by PCR.…”

Section: Populationmentioning

confidence: 97%

Bayesian analysis of culture and PCR methods for detection of Campylobacter spp. in broiler caecal samples

et al. 2014

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The objective of this study was to estimate the sensitivity and specificity of a culture method and a polymerase chain reaction (PCR) method for detection of two Campylobacter species: C. jejuni and C. coli. Data were collected during a 3-year survey of UK broiler flocks, and consisted of parallel sampling of caeca from 436 batches of birds by both PCR and culture. Batches were stratified by season (summer/non-summer) and whether they were the first depopulation of the flock, resulting in four sub-populations. A Bayesian approach in the absence of a gold standard was adopted, and the sensitivity and specificity of the PCR and culture for each Campylobacter subtype was estimated, along with the true C. jejuni and C. coli prevalence in each sub-population. Results indicated that the sensitivity of the culture method was higher than that of PCR in detecting both species when the samples were derived from populations infected with at most one species of Campylobacter. However, from a mixed population, the sensitivity of culture for detecting both C. jejuni or C. coli is reduced while PCR is potentially able to detect both species, although the total probability of correctly identifying at least one species by PCR is similar to that of the culture method.

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An evaluation of survival and detection of Campylobacter jejuni and C. coli in broiler caecal contents using culture-based methods

Cited by 20 publications

References 40 publications

Campylobacter populations in wild and domesticated Mallard ducks (Anas platyrhynchos)

Campylobacter populations in wild and domesticated Mallard ducks (Anas platyrhynchos)

Comparison of Different Sampling Strategies and Laboratory Methods for the Detection of C. jejuni and C. coli from Broiler Flocks at Primary Production

Bayesian analysis of culture and PCR methods for detection of Campylobacter spp. in broiler caecal samples

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