Laboratory assessment of entomopathogenic nematode symbiotic bacteria to control maize pest, Ostrinia furnacalis, and fungi diseases, Bipolaris maydis and Curvularia lunata

Wang, Huan; Dong, Hui; HaiTao, Qian; Cong, Bin

doi:10.1016/j.aspen.2014.08.002

Cited by 5 publications

(4 citation statements)

References 24 publications

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“…Chinese Xenorhabdus sp. X. nematophila displayed antifungal activity against maize fungal pathogens Bipolarismaydis and Curvularialunata with inhibition rates of 66.7% and 69.1%, respectively [34]. The results we obtained from our Xenorhabdus sp.…”

Section: Discussionmentioning

confidence: 51%

“…The enzymes involved in production of these metabolites are polyketide synthetases (PRSs), nonribosomal peptide synthetases (NRPSs), and other similar enzymes [50]. Wang et al [34] also reported a decline in the inhibition activity of the metabolites against Bipolaris maydis after their exposure to high temperatures (50 • C and 100 • C). However, the same metabolites did not lose their inhibition activity against Curvularia lunata after their exposure to high temperatures.…”

Section: Discussionmentioning

confidence: 99%

“…live in symbiosis with EPNs of the family Heterorhabbditidae and Steinernematidae, respectively [33]. These bacterial symbionts are released from their respective EPN's infective juvenile into the insect's hemocoel, where they reproduce and kill the insect host rapidly within 48 h [34]. Photorhabdus and Xenorhabdus spp.…”

Section: Introductionmentioning

confidence: 99%

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Antifungal Effect of Metabolites from Bacterial Symbionts of Entomopathogenic Nematodes on Fusarium Head Blight of Wheat

Kgosiemang,

Ramakuwela,

Figlan

et al. 2024

JoF

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Fungal diseases such as Fusarium head blight (FHB) are significant biotic stressors, negatively affecting wheat production and quality. This study explored the antifungal activity of the metabolites produced by the bacterial symbionts of entomopathogenic nematodes (EPNs) against FHB-causing Fusarium sp. Fusarium graminearum. To achieve this, the symbiotic bacteria of nine EPN isolates from the EPN collection at the Agricultural Research Council-Small Grains (ARC-SG) were isolated from the cadavers of Galleria mellonella (Lepidoptera: Pyralidae) larvae after infection with EPNs. Broth cultures (crude) and their supernatants (filtered and autoclaved) of each bacterial isolate were used as bacterial metabolite treatments to test their inhibitory effect on the mycelial growth and spore germination of F. graminearum. Mycelial growth inhibition rates varied among both bacterial isolates and treatments. Crude metabolite treatments proved to be more effective than filtered and autoclaved metabolite treatments, with an overall inhibition rate of 75.25% compared to 23.93% and 13.32%, respectively. From the crude metabolite treatments, the Xenorhabdus khoisanae SGI 197 bacterial isolate from Steinernema beitlechemi SGI 197 had the highest mean inhibition rate of 96.25%, followed by Photorhabdus luminescens SGI 170 bacteria isolated from Heterorhabditis bacteriophora SGI 170 with a 95.79% mean inhibition rate. The filtered metabolite treatments of all bacterial isolates were tested for their inhibitory activity against Fusarium graminearum spore germination. Mean spore germination inhibition rates from Xenorhabdus spp. bacterial isolates were higher (83.91 to 96.29%) than those from Photorhabdus spp. (6.05 to 14.74%). The results obtained from this study suggest that EPN symbiotic bacterial metabolites have potential use as biological control agents of FHB. Although field efficacy against FHB was not studied, the significant inhibition of mycelial growth and spore germination suggest that the application of these metabolites at the flowering stage may provide protection to plants against infection with or spread of F. graminearum. These metabolites have the potential to be employed as part of integrated pest management (IPM) to inhibit/delay conidia germination until the anthesis (flowering stage) of wheat seedlings has passed.

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Section: Discussionmentioning

confidence: 51%

Section: Discussionmentioning

confidence: 99%

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Antifungal Effect of Metabolites from Bacterial Symbionts of Entomopathogenic Nematodes on Fusarium Head Blight of Wheat

Kgosiemang,

Ramakuwela,

Figlan

et al. 2024

JoF

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“…The sequences most closely related to BmDODAs are from fungi, and most sequences belong to Ascomycota. Many lepidopteran species have been reported to carry these pathogenic fungi (Hussain et al, 2009;Quesada-Moraga et al, 2013;Wang et al, 2014;Pestano et al, 2017). The physical interaction between the host and pathogen mostly likely promotes HGT from fungi to lepidopteran insects.…”

Section: Phylogeny Of Bmdoda Genes and Hgt Events From Fungimentioning

confidence: 99%

Functional characterization of the horizontally transferred 4,5‐DOPA extradiol dioxygenase gene in the domestic silkworm, Bombyx mori

Wang

Sun

Zhang

2019

Insect Molecular Biology

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4,5‐DOPA dioxygenase (DODA) is a crucial enzyme in the biosynthetic pathway of betalain. Previous studies have shown that DODA is present in plants, fungi and bacteria. Using updated data, here we show that DODA genes (BmDODA) in the domestic silkworm (Bombyx mori) and other lepidopteran insects are most likely to be horizontally transferred from fungi. A synteny analysis indicated that BmDODA1 is orthologous to other lepidopteran DODAs and that BmDODA2 is a paralogous gene. To explore the function of DODA in Lepidoptera, we first examined the expression patterns of BmDODA1. BmDODA1 showed high transcriptional and translational levels in the midgut and head. Then, we exogenously expressed the BmDODA1 gene, detected 4,5‐DOPA ring‐cleaving activity and calculated the kinetic parameters of the recombinant BmDODA1. We found that the transcription levels of BmDODA1 were significantly induced by the pathogens Bacillus bombyseptieus and Escherichia coli. Thus, the horizontal transfer of the BmDODA gene in the silkworm may be involved in dopa metabolism and contribute to antimicrobial activity in this species. Our results provide a documented example of functional horizontal gene transfer (HGT) between fungi and animals and expand our knowledge of HGT amongst eukaryotes.

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The application of entomopathogenic nematode modified microbial communities within nesting mounds of the red imported fire ants, Solenopsis invicta

Li,

Yi,

Chen

et al. 2024

Science of The Total Environment

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Laboratory assessment of entomopathogenic nematode symbiotic bacteria to control maize pest, Ostrinia furnacalis, and fungi diseases, Bipolaris maydis and Curvularia lunata

Cited by 5 publications

References 24 publications

Antifungal Effect of Metabolites from Bacterial Symbionts of Entomopathogenic Nematodes on Fusarium Head Blight of Wheat

Antifungal Effect of Metabolites from Bacterial Symbionts of Entomopathogenic Nematodes on Fusarium Head Blight of Wheat

Functional characterization of the horizontally transferred 4,5‐DOPA extradiol dioxygenase gene in the domestic silkworm, Bombyx mori

The application of entomopathogenic nematode modified microbial communities within nesting mounds of the red imported fire ants, Solenopsis invicta

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