Laboratory bioassays on the response of honey bee (Apis mellifera L.) glutathione S-transferase and acetylcholinesterase to the oral exposure to copper, cadmium, and lead

Nikolić, Tatjana; Kojić, Danijela; Orčić, Snežana; Vukašinović, Elvira L.; Blagojević, Duško; Purać, Jelena

doi:10.1007/s11356-018-3950-6

Cited by 23 publications

(13 citation statements)

References 61 publications

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“…AChE and TP levels after 48 h exposure. Honeybees (three per vial) were collected and transferred into plastic vials (5 mL) which were immediately frozen in dry ice and stored in subsequent analysis (Nikolic et al ., 2019). Wings and legs were removed.…”

Section: Methodsmentioning

confidence: 99%

A study on lethal doses of various pesticides on honeybees (Apis mellifera L.) – a laboratory trial

Pervez

Manzoor

2020

Physiol. Entomol.

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Exposure of honeybee (Apis mellifera L.) to pesticides disturbed normal physiological and behavioral functions required for normal foraging and colony maintenance. The present study was aimed towards establishing the mean lethal concentration (LC50) of three pesticides viz. carbamate (carbaryl), organophosphate (chlorpyrifos) and neonicotinoid (imidacloprid) in honeybees through feeding bioassay laboratory trial. Deleteriousness was confirmed through mortality rate, number of survival bees, acetylcholinesterase (AChE), total protein (TP) levels, AChE gene transcription level, and gut tissues histological analysis of exposed honeybees. Mean mortality rate was calculated for 96 h interval at three different concentrations of tested pesticides (5, 2.5, and 1.25 mg L−1), and LC50 values calculated for 48 and 96 h interval. AChE enzyme and TP level are determined by ELIZA and spectrophotometer, respectively. Results revealed that imidacloprid had the lowest LC50 (0.477 ng/bee) values as compared with carbaryl and chlorpyrifos. High mortality rate was observed at highest dose, being impidacloprid have more lethal effects as compared with other pesticides. Similarly, biochemical analysis revealed that imidiacloprid and chlorpyrifos significantly (P ≤ 0.05) increased AChE and TP levels, whereas carbaryl significantly (P ≤ 0.05) decreased them. Similarly, probe based RT qPCR revealed that imidacloprid and chlorpyrifos treatments significantly (P ≤ 0.05) enhanced the AChE level whereas carbaryl decreased it. Histological analysis showed that the gut tissues of honeybees exposed to pesticide treatment had substantial morphological abnormalities. In a nutshell, imidacloprid, carbaryl and chlorpyrifos have substantial toxic effects on all the study attributes of honeybees with imidacloprid being most toxic.

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Section: Methodsmentioning

confidence: 99%

A study on lethal doses of various pesticides on honeybees (Apis mellifera L.) – a laboratory trial

Pervez

Manzoor

2020

Physiol. Entomol.

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“…For example, at the organismal level, exposure to manganese increases bee foraging time (6), exposure to copper affects feeding behavior (55), and exposure to imidacloprid alters nest behaviors (56). Metabolically, exposing bees to heavy metals increases detoxification enzyme activity (57,58) and metallothionein-like protein levels (59) while affecting their overall redox system (60), which may indicate a general response to toxic metal stress. Similarly, metals have been shown to hamper immunocompetence in bees (61), ants (62), and moths (63).…”

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confidence: 99%

Cadmium and Selenate Exposure Affects the Honey Bee Microbiome and Metabolome, and Bee-Associated Bacteria Show Potential for Bioaccumulation

Rothman

Leger

Kirkwood

et al. 2019

Appl Environ Microbiol

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Honey bees are important insect pollinators used heavily in agriculture and can be found in diverse environments. Bees may encounter toxicants such as cadmium and selenate by foraging on plants growing in contaminated areas, which can result in negative health effects. Honey bees are known to have a simple and consistent microbiome that conveys many benefits to the host, and toxicant exposure may impact this symbiotic microbial community. We used 16S rRNA gene sequencing to assay the effects that sublethal cadmium and selenate treatments had over 7 days and found that both treatments significantly but subtly altered the composition of the bee microbiome. Next, we exposed bees to cadmium and selenate and then used untargeted liquid chromatography-mass spectrometry (LC-MS) metabolomics to show that chemical exposure changed the bees' metabolite profiles and that compounds which may be involved in detoxification, proteolysis, and lipolysis were more abundant in treatments. Finally, we exposed several strains of bee-associated bacteria in liquid culture and found that each strain removed cadmium from its medium but that only Lactobacillus Firm-5 microbes assimilated selenate, indicating the possibility that these microbes may reduce the metal and metalloid burden on their host. Overall, our report shows that metal and metalloid exposure can affect the honey bee microbiome and metabolome and that strains of bee-associated bacteria can bioaccumulate these toxicants. IMPORTANCE Bees are important insect pollinators that may encounter environmental pollution when foraging upon plants grown in contaminated areas. Despite the pervasiveness of pollution, little is known about the effects of these toxicants on honey bee metabolism and their symbiotic microbiomes. Here, we investigated the impact of selenate and cadmium exposure on the gut microbiome and metabolome of honey bees. We found that exposure to these chemicals subtly altered the overall composition of the bees' microbiome and metabolome and that exposure to toxicants may negatively impact both host and microbe. As the microbiome of animals can reduce mortality upon metal or metalloid challenge, we grew bee-associated bacteria in media spiked with selenate or cadmium. We show that some bacteria can remove these toxicants from their media in vitro and suggest that bacteria may reduce metal burden in their hosts.

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“…Our results may indicate that bees from both apiaries were not seriously affected by pesticides, at least those from the neonicotinoid group. All the more so because artificial feeding of honey bees workers with different concentrations of Cd, Pb, and Cu in sucrose solutions changed GST and AChE activity and increased level of gene expression of the three classes of GST with increasing concentrations of Cu and Cd (Nikolić et al 2019 ).…”

Section: Discussionmentioning

confidence: 99%

“…The AChE activity may indicate that an animal was exposed to organophosphorus or carbamate insecticides (Nauen et al 2001 ). GST and AChE response to insecticides was presented under exposure of four insecticides in individuals of A. mellifera (Badawy et al 2015 ) and heavy metals (Nikolić et al 2019 ). New data has indicated that AChE may be involved in the manifold stress response or stress management (Kim et al 2017 ).…”

Section: Introductionmentioning

confidence: 99%

Set of stress biomarkers as a practical tool in the assessment of multistress effect using honeybees from urban and rural areas as a model organism: a pilot study

Nicewicz

Kafel

et al. 2020

Environ Sci Pollut Res

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A decrease among honey bee populations (Apis mellifera) in the traditional apiaries has been observed in recent years. In light of this negative phenomenon, urban beekeeping seems to be an appropriate alternative solution for the bee population in reducing the toxic effects of a large number of pesticides that are commonly used in agricultural ecosystems. Despite the rapid development of urban beekeeping, there is little information regarding the different aspects of the defense effectiveness of bees from the urban and rural areas. The study was aimed to show whether honey bees from these two locations differ in the level of the valuable biomarkers of stress exposure helpful in establishing which bees, from urban or rural areas, are under greater environmental pressure. For this purpose, foragers from an urban rooftop apiary and a traditional rural apiary were collected. The chosen biomarkers were measured in various tissues of bees. The activity of glutathione S-transferase and acetylcholinesterase, the level of total antioxidant capacity, heat shock protein 70 (Hsp70), and defensin were selected for the analyses. In our opinion, the Hsp70 and defensin levels seemed to be important in the indication of urban multistress factors. The higher level of heat shock proteins and defensins in tissues/organs of bees from the urban apiary—in the gut (an increase, respectively, 92% and 7.3%) and fat body (an increase, respectively, 130% and 7.8%), known as targets of environmental toxins, pointed out the urban environment as highly stressful at both the individual and colony levels. In turn, high total antioxidant capacity was measured in the guts of honey bees from rural area (an increase 107%). Such a situation suggests a different mechanism of defense and specificity of rural and urban environmental stressors and also honey bees foraging activity.

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Laboratory bioassays on the response of honey bee (Apis mellifera L.) glutathione S-transferase and acetylcholinesterase to the oral exposure to copper, cadmium, and lead

Cited by 23 publications

References 61 publications

A study on lethal doses of various pesticides on honeybees (Apis mellifera L.) – a laboratory trial

A study on lethal doses of various pesticides on honeybees (Apis mellifera L.) – a laboratory trial

Cadmium and Selenate Exposure Affects the Honey Bee Microbiome and Metabolome, and Bee-Associated Bacteria Show Potential for Bioaccumulation

Set of stress biomarkers as a practical tool in the assessment of multistress effect using honeybees from urban and rural areas as a model organism: a pilot study

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