Laboratory diagnostics for monkeypox: An overview of sensitivities from various published tests

Jiang, Zhiwen; Sun, Jixiang; Zhang, Letian; Lai, Alexander; su, Shuo

doi:10.1016/j.tmaid.2022.102425

Cited by 29 publications

(23 citation statements)

References 6 publications

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“… 290 With different target sites in the viral genome, these tests have varying sensitivities and limits of detection for monkeypox virus. 291 According to Li et al 278 real-time PCR assay for generic monkeypox virus has a limit of detection for 0.7 fg (∼3.5 genomes), while monkeypox virus West Africa specific (G2R_WA) assay’s LOD is at 1.7 fg (∼8.2 genome), and monkeypox virus Congo Basin (C3L) assay’s LOD is at 9.46 fg (∼40.4 genomes). As well, Maksyutov et al 292 developed real-time PCR assay that targets viral F3L gene with a LOD of 20 copies per reaction.…”

Section: Laboratory Diagnosismentioning

confidence: 99%

Monkeypox: epidemiology, pathogenesis, treatment and prevention

Huang

Wang

2022

Sig Transduct Target Ther

162

120

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Monkeypox is a zoonotic disease that was once endemic in west and central Africa caused by monkeypox virus. However, cases recently have been confirmed in many nonendemic countries outside of Africa. WHO declared the ongoing monkeypox outbreak to be a public health emergency of international concern on July 23, 2022, in the context of the COVID-19 pandemic. The rapidly increasing number of confirmed cases could pose a threat to the international community. Here, we review the epidemiology of monkeypox, monkeypox virus reservoirs, novel transmission patterns, mutations and mechanisms of viral infection, clinical characteristics, laboratory diagnosis and treatment measures. In addition, strategies for the prevention, such as vaccination of smallpox vaccine, is also included. Current epidemiological data indicate that high frequency of human-to-human transmission could lead to further outbreaks, especially among men who have sex with men. The development of antiviral drugs and vaccines against monkeypox virus is urgently needed, despite some therapeutic effects of currently used drugs in the clinic. We provide useful information to improve the understanding of monkeypox virus and give guidance for the government and relative agency to prevent and control the further spread of monkeypox virus.

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Section: Laboratory Diagnosismentioning

confidence: 99%

Monkeypox: epidemiology, pathogenesis, treatment and prevention

Huang

Wang

2022

Sig Transduct Target Ther

162

120

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“…PCR tests are highly sensitive, being able to detect as little as 10 viral genomes, and highly specific. 18 , 19 …”

Section: Diagnosismentioning

confidence: 99%

Monkeypox (hMPXV Infection): A Practical Review

Salcedo¹,

Madariaga²

2023

The American Journal of Medicine

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“…Diagnostic/testing capacity for monkeypox varies substantially worldwide-some countries like the United States are able to process up to several thousand specimens per week (Cohen, 2022), while others have no diagnostic capacity at all; moreover, testing and diagnostic capacity are further strained by the still ongoing COVID-19 pandemic. Testing includes non-variola Orthopoxvirus (NVO) generic real-time polymerase chain reaction (PCR) test, monkeypox-specific PCR, and sequencing (Jiang et al, 2022).…”

Section: Underestimation Of Monkeypox Casesmentioning

confidence: 99%

Knowing the unknown: The underestimation of monkeypox cases. Insights and implications from an integrative review of the literature

Bragazzi

Woldegerima²,

Iyaniwura³

et al. 2022

Front. Microbiol.

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Monkeypox is an emerging zoonotic disease caused by the monkeypox virus, which is an infectious agent belonging to the genus Orthopoxvirus. Currently, commencing from the end of April 2022, an outbreak of monkeypox is ongoing, with more than 43,000 cases reported as of 23 August 2022, involving 99 countries and territories across all the six World Health Organization (WHO) regions. On 23 July 2022, the Director-General of the WHO declared monkeypox a global public health emergency of international concern (PHEIC), since the outbreak represents an extraordinary, unusual, and unexpected event that poses a significant risk for international spread, requiring an immediate, coordinated international response. However, the real magnitude of the burden of disease could be masked by failures in ascertainment and under-detection. As such, underestimation affects the efficiency and reliability of surveillance and notification systems and compromises the possibility of making informed and evidence-based policy decisions in terms of the adoption and implementation of ad hoc adequate preventive measures. In this review, synthesizing 53 papers, we summarize the determinants of the underestimation of sexually transmitted diseases, in general, and, in particular, monkeypox, in terms of all their various components and dimensions (under-ascertainment, underreporting, under-detection, under-diagnosis, misdiagnosis/misclassification, and under-notification).

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Laboratory diagnostics for monkeypox: An overview of sensitivities from various published tests

Cited by 29 publications

References 6 publications

Monkeypox: epidemiology, pathogenesis, treatment and prevention

Monkeypox: epidemiology, pathogenesis, treatment and prevention

Monkeypox (hMPXV Infection): A Practical Review

Knowing the unknown: The underestimation of monkeypox cases. Insights and implications from an integrative review of the literature

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