Laboratory Evaluation of Beauveria bassiana ARP14 Against Grapholita molesta (Lepidoptera: Tortricidae)

Sarker, Souvic; Woo, Young Ha; Lim, Un Taek

doi:10.1007/s00284-020-02012-4

Cited by 11 publications

(10 citation statements)

References 32 publications

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“…Other studies have evaluated the pathogenicity of B. bassiana strain ARP14 to larvae and adults of G. molesta, but did not test the susceptibility of G. molesta eggs. 26 Possibly, the chorion presents a hard physical barrier that impedes penetration of B. bassiana appressoria. Similarly, eggs of Tetranychus urticae Koch are much less susceptible to B. bassiana than later life stages.…”

Section: Discussionmentioning

confidence: 99%

“…Female moths that survived treatments with concentrations of 2.5 × 10 7 conidial mL −1 or higher had significantly reduced fecundity, indicating they would contribute less to the subsequent generation and cause less fruit damage. Other studies have evaluated the pathogenicity of B. bassiana strain ARP14 to larvae and adults of G. molesta , but did not test the susceptibility of G. molesta eggs 26 . Possibly, the chorion presents a hard physical barrier that impedes penetration of B. bassiana appressoria.…”

Section: Discussionmentioning

confidence: 99%

“…Suspensions of 2.5 × 10 5 to 2.5 × 10 9 B. bassiana conidia mL −1 were prepared (as above) and sprayed on 1‐day‐old adults (of both sexes) in Petri dishes (9.0 cm diameter), with 0.05% Tween 80 solution serving as a control. Ten minutes later, moths were transferred to ventilated plastic containers (as above, 20 adults per container) and held under the same conditions as the stock colony 26 . The number of dead moths was recorded daily for 14 days.…”

Section: Methodsmentioning

confidence: 99%

“…Ten minutes later, moths were transferred to ventilated plastic containers (as above, 20 adults per container) and held under the same conditions as the stock colony. 26 The number of dead moths was recorded daily for 14 days.…”

Section: B Bassiana Toxicity Assays 231 Grapholita Molestamentioning

confidence: 99%

“…18,19 The generalist EPF Beauveria bassiana (Balsamo) has been widely used in vegetable and orchard pest control via direct application in the field and also via parasitoid-mediated dissemination. [20][21][22][23][24][25] Laboratory evaluation showed that B. bassiana strain ARP14 was virulent against G. molesta first instar larvae and adults, 26 and recent work has illustrated its pathogenicity to insect eggs, which it can infect through chorion in a process similar to those used to penetrate the cuticle. [27][28][29][30] Therefore, we explored the compatibility of Trichogramma parasitoids and B. bassiana for potential combined application to enhance the integrated management of G. molesta.…”

Section: Introductionmentioning

confidence: 99%

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Laboratory evaluation of the compatibility of Beauveria bassiana with the egg parasitoid Trichogramma dendrolimi (Hymenoptera: Trichogrammatidae) for joint application against the oriental fruit moth Grapholita molesta (Lepidoptera: Tortricidae)

Fang

Liu

et al. 2022

Pest Management Science

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BACKGROUND The entomopathogenic fungus Beauveria bassiana and the egg parasitoid Trichogramma dendrolimi can both contribute to biological control of the global fruit borer Grapholita molesta. To derive insights for optimizing their combined application in augmentation programs, we assayed fungal pathogenicity to both G. molesta (eggs, larvae and adults) and adult parasitoids, and assessed wasp acquisition and transmission of fungal spores following their emergence from B. bassiana‐treated host eggs. RESULTS Concentrations of 108 conidia mL−1 of B. bassiana and higher caused virtually complete mortality of G. molesta larvae, but less than 30% mortality of eggs, and female moths surviving treatments had reduced fecundity. More than 80% of adult T. dendrolimi survived B. bassiana treatments of 107 conidia mL−1, and more than 60% survived 109 conidia mL−1, with surviving females achieving reproductive success equivalent to controls. Parasitoid females preferred healthy eggs over B. bassiana‐infected ones in choice tests; wasps emerging from treated eggs carried about 104 conidia each and contributed an additional 11% host mortality in trials combining both agents. CONCLUSION Our results indicate that combined applications of B. bassiana and T. dendrolimi can have complimentary impacts on G. molesta, the wasps compensating for low fungal pathogenicity to eggs and their progeny potentially aiding in subsequent disease transmission. Although foraging wasps tended to avoid infected eggs, negative interactions between the two agents might be further mitigated by timing B. bassiana applications from late instar larva to early moth stage, and T. dendrolimi releases several days later to coincide with peak oviposition periods. © 2022 Society of Chemical Industry.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: B Bassiana Toxicity Assays 231 Grapholita Molestamentioning

confidence: 99%