Laboratory exposure to 17β-estradiol fails to induce vitellogenin and estrogen receptor gene expression in the marine invertebrate Mytilus edulis

Puinean, Alin M.; Labadie, Pierre; Hill, Elizabeth M.; Osada, Makoto; Kishida, Michiko; Nakao, Reiko; Novillo, Apolonia; Callard, İan P.; Rotchell, Jeanette M.

doi:10.1016/j.aquatox.2006.07.006

Cited by 76 publications

(35 citation statements)

References 20 publications

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“…The fact that males also exhibit this vitellogenin induction, suggests estrogens exert a response not observed in unexposed males, indicative of endocrine disruption. Indeed, male molluscs (E. complanata and M. edulis) have been shown to possess a functional, yet silent, gene for vitellogenin which has been shown to be activated upon exposure to exogenous estrogens in E. complanata (Gagné et al 2005) but not in M. edulis (Puinean et al 2006). Further, others have found that male molluscs can exhibit sensitivity to estrogenic exposure in terms of initiation of vitellogenesis (Matozzo and Marin 2005;Blaise et al 1999Blaise et al , 2003.…”

Section: Discussionmentioning

confidence: 93%

“…However, in the resting phase, they found that ALP significantly increased in the hemolymph of females following 7 days exposure to E2 (5, 25, 50, 100 and 1,000 ng/l), but ALP levels in exposure treatments had declined following 14 days and were only significant at the highest exposure (1,000 ng/l) ). Similarly, Puinean et al (2006) found that Blue mussels, Mytilus edulis exposed to 200 ng/l E2 displayed no significant differences in vitellogenin gene expression compared to control individuals during the mature stages of gametogenesis. Taken together, these observations imply vitellogenin is most actively synthesised, and most responsive to estrogenic stimulation, during earlier phases of gonadal development, suggesting individuals commencing a gonadal development cycle should be targeted when employing vitellogenin as a biomarker of estrogenic exposure in S. glomerata.…”

Section: Discussionmentioning

confidence: 93%

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Exposure to 17α-ethynylestradiol causes dose and temporally dependent changes in intersex, females and vitellogenin production in the Sydney rock oyster

et al. 2010

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Although mounting evidence suggests exposure to estrogenic contaminants increases vitellogenin production in molluscs, demonstration of dose-response relationships and knowledge of the temporal nature of the vitellogenin response with continual exposure is currently lacking for biomarker utility. To address this knowledge gap, adult Sydney rock oysters, Saccostrea glomerata, were exposed to a range of environmentally relevant concentrations of 17α-ethynylestradiol (EE2) (0, 6.25, 12.5, 25 or 50 ng/l) in seawater under laboratory conditions. Vitellogenin induction and gonadal development was assessed following 4, 21 and 49 days exposure to EE2. Vitellogenin was found to increase in a dose dependent manner with EE2 exposure for females (4 and 49 days) and males (4 and 21 days). Histological examination of gonads revealed a number of individuals exhibited intersex (ovotestis) in 50 ng/l EE2 (after 21 days) and in 6.25 and 12.5 ng/l EE2 (after 49 days). Furthermore, a significant shift towards females was observed following 49 days exposure at 50 ng/l EE2 suggesting estrogenic exposure is capable of facilitating a progression for protandric males from male-intersex-female gametal status. Increases in female vitellogenin (4 days) were predictive of later increases in female developmental stages at 21 days and increases in oocyte area following 49 days. Male vitellogenin (4 days) was predictive of decreased male percentages and lower male developmental stages at 49 days. Vitellogenin in S. glomerata is a predictive biomarker of estrogenic exposure and effect if sampled soon after exposure and at the commencement of a gonadal development cycle.

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Section: Discussionmentioning

confidence: 93%

Section: Discussionmentioning

confidence: 93%

Exposure to 17α-ethynylestradiol causes dose and temporally dependent changes in intersex, females and vitellogenin production in the Sydney rock oyster

et al. 2010

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“…In these reports, the regulatory mechanisms of mRNA expression and protein synthesis of VTG in C. picta were studied in detail, but the nucleotide sequence of the turtle VTG has not yet been clarified. There are numerous reports concerning the mRNA expression of VTG which investigate the effects of estrogens through reverse transcriptionpolymerase chain reaction (RT-PCR) in birds [25], zebrafish [22], so-iuy mullet [1], and marine mussel [28]. In turtles, however, only northern blotting analysis has been developed [5,[7][8][9][10].…”

mentioning

confidence: 99%

Vitellogenin, a Biomarker for Environmental Estrogenic Pollution, of Reeves' Pond Turtles: Analysis of Similarity for its Amino Acid Sequence and Cognate mRNA Expression after Exposure to Estrogen

Tada

Nakao

Hoshi

et al. 2008

The Journal of Veterinary Medical Science

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ABSTRACT. Vitellogenin (VTG), a biomarker for environmental estrogenic pollution, can be detected in the bloodstream of oviparous animals before morphological and functional abnormalities appear due to exposure to environmental estrogens. Reports observing VTG in turtles have been limited. We therefore cloned and sequenced a partial cDNA of VTG in Reeves' pond turtle, Chinemys reevesii. The cloned cDNA fragment possessed the start codon and 2,229 bp, encoding 743 amino acid residues. A sequence of deduced amino acid from the cDNA did not contain a high serine content, such as that which exists in phosvitin. Two N-glycosylation sites were found in the sequence. The sequence was compared to those of two birds (chicken and herring gull), one amphibian (Xenopus), and five fishes (carp, zebrafish, eel, haddock, and red seabream). The C. reevesii VTG was similar to that of herring gull (78%, value of positives), chicken (76%), Xenopus (69%), eel (63%), red seabream (62%), haddock (62%), carp (62%), and zebrafish (61%). The phylogenetic tree showed that C. reevesii VTG existed between the amphibian and birds, and it was present far from fish VTGs. A reverse transcription-polymerase chain reaction method was employed to detect the mRNA expression of the C. reevesii VTG through the use of primers designed from our sequence. The VTG mRNA expression (292 bp) was proven in the total RNA extraction from the liver of the juvenile turtles which were treated with estradiol-17β. The information herein would be useful for ecotoxicological studies using freshwater turtles and these findings are expected to contribute positively towards wildlife conservation.

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“…EDCs will disrupt reproduction activity through interruption of ER-mediated processes. The principal result of the present investigation is that continuous exposure to all concentrations of E-2 tested severely affected reproduction, sexual development and survival [12,31]. Evidence indicates that CPs from the environment might have oestrogenic activities [12,15,31].…”

Section: Discussionmentioning

confidence: 86%

Comparative proteomic analysis of ovary for Chinese rare minnow (Gobiocypris rarus) exposed to chlorophenol chemicals

Fang¹,

Gao

Zhao

et al. 2014

Journal of Proteomics

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Laboratory exposure to 17β-estradiol fails to induce vitellogenin and estrogen receptor gene expression in the marine invertebrate Mytilus edulis

Cited by 76 publications

References 20 publications

Exposure to 17α-ethynylestradiol causes dose and temporally dependent changes in intersex, females and vitellogenin production in the Sydney rock oyster

Exposure to 17α-ethynylestradiol causes dose and temporally dependent changes in intersex, females and vitellogenin production in the Sydney rock oyster

Vitellogenin, a Biomarker for Environmental Estrogenic Pollution, of Reeves' Pond Turtles: Analysis of Similarity for its Amino Acid Sequence and Cognate mRNA Expression after Exposure to Estrogen

Comparative proteomic analysis of ovary for Chinese rare minnow (Gobiocypris rarus) exposed to chlorophenol chemicals

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